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Full-Text Articles in Biotechnology
Sterol Carrier Protein-2 Directly Interacts With Caveolin-1 In Vitro And In Vivo, Minglong Zhou, Rebecca D. Parr, Anca D. Petrescu, H. Ross Payne, Barbara P. Atshaves, Ann B. Kier, Judith M. Ball, Friedhelm Schroeder
Sterol Carrier Protein-2 Directly Interacts With Caveolin-1 In Vitro And In Vivo, Minglong Zhou, Rebecca D. Parr, Anca D. Petrescu, H. Ross Payne, Barbara P. Atshaves, Ann B. Kier, Judith M. Ball, Friedhelm Schroeder
Faculty Publications
HDL-mediated reverse-cholesterol transport as well as phosphoinositide signaling are mediated through plasma membrane microdomains termed caveolae/lipid rafts. However, relatively little is known regarding mechanism(s) whereby these lipids traffic to or are targeted to caveolae/lipid rafts. Since sterol carrier protein-2 (SCP-2) binds both cholesterol and phosphatidylinositol, the possibility that SCP-2 might interact with caveolin-1 and caveolae was examined. Double immunolabeling and laser scanning fluorescence microscopy showed that a small but significant portion of SCP-2 colocalized with caveolin-1 primarily at the plasma membrane of L-cells and more so within intracellular punctuate structures in hepatoma cells. In SCP-2 overexpressing L-cells, SCP-2 was detected …