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Somatic Cell Nuclear Transfer In Zebrafish, Kannika Siripattarapravat, Patrick J. Venta, C C. Chang, Jose B. Cibelli
Somatic Cell Nuclear Transfer In Zebrafish, Kannika Siripattarapravat, Patrick J. Venta, C C. Chang, Jose B. Cibelli
Jose Cibelli
We developed a method for somatic cell nuclear transfer in zebrafish using laser-ablated metaphase II eggs as recipients, the micropyle for transfer of the nucleus and an egg activation protocol after nuclear reconstruction. We produced clones from cells of both embryonic and adult origins, although the latter did not give rise to live adult clones.