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- Aegle marmelos (5)
- Micropropagation (3)
- Bael (2)
- Genetic transformation (2)
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- Histology (2)
- In vitro (2)
- Mango (2)
- Polyembryonic (2)
- Somatic embryogenesis (2)
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- Agrobacterium tumefaciens (1)
- Allahabad safeda (1)
- Alpinia/*chemistry (1)
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- Ammi majus (1)
- Anatomy (1)
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- Antineoplastic Agents/isolation & purification/*pharmacology (1)
- Antioxidant activity (1)
- Apoptosis/*drug effects (1)
- Asparagus officinalis (1)
- Benzyl Alcohols/isolation & purification/*pharmacology (1)
- Biochemical (1)
- Biotic (1)
- CISH-B1 (1)
- Cell Cycle/*drug effects (1)
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Articles 1 - 19 of 19
Full-Text Articles in Biotechnology
Progress And Future Prospects Of Developing Transgenics In Mango, Rajesh Pati
Progress And Future Prospects Of Developing Transgenics In Mango, Rajesh Pati
Rajesh Pati
Mango (Mangifera indica L.) is the most important fruit crop of the world. India ranks first among world’s mango producing countries accounting for 57.18 % of the total world mango production of 19.22 million tones. With the development of rDNA technology, plant breeders have access to a large number of genes that can be integrated into the plant genome.
Histological And Biochemical Changes In Aegle Marmelos, Rajesh Pati
Histological And Biochemical Changes In Aegle Marmelos, Rajesh Pati
Rajesh Pati
Aegle marmelos Corr., belongs to family Rutaceae, is more prized for its pharmacological virtues than its edible quality. Because of pharmacological importance, it’s become potential candidate for developing transgenics to enhance its medicinal properties. Maximum mortality of micropropagated plants occur during acclimatization phase because plantlets undergo rapid and extreme changes in physiological functioning, histological and biochemical changes. In order to investigate the actual reason of this limitation, test samples were collected at different stages of micropropagation of Aegle marmelos Corr. (In vitro stage, acclimation stage, and field established plants).
Somatic Embryogenesis In Mono And Poly Embryonic Cultivars, Rajesh Pati
Somatic Embryogenesis In Mono And Poly Embryonic Cultivars, Rajesh Pati
Rajesh Pati
A standard uniform protocol of regeneration is the prime and foremost prerequisite for not only improve the productivity, the production from the existing area but also development of transgenics in mango for various traits. Polyembryonic cultivars are good rootstock because of moderate canopy, tolerant to draught, salt, environmental stress (wind, high temperature, air pollution), fungal disease (anthracnose, powdery mildew, gummosis) and hoppers.
Genetic Transformation Of Bael (Aegle Marmelos Corr.), Rajesh Pati
Genetic Transformation Of Bael (Aegle Marmelos Corr.), Rajesh Pati
Rajesh Pati
Aegle marmelos Corr. is better known for its medicinal values rather than its edible quality. Every part of the plant viz. leaves, fruits, roots, and the bark are used for treating various diseases related to heart, stomach, bacterial, fungal, viral, worms, fertility, fever and cosmetics. Marmelosin is one of the major compounds (coumarin), used to treat various diseases. Till date, there are no varieties developed for commercial orchards of Bael and hence, regarded as one among the underutilized fruits of India. Therefore, there is an urgent need for breeding new and cultivable varieties in Aegle marmelos. But the problem for …
Micropropagation In Fruit Crops, Rajesh Pati
Micropropagation In Fruit Crops, Rajesh Pati
Rajesh Pati
Micropropagation is a proven means of producing millions of identical plants under a controlled and aseptic condition, independent of seasonal constraints. It not only provides economy of time and space but also gives greater output and allows further augmentation of elite disease free propagules. India is homeland of many important fruit crops such as Indian gooseberry (Emblica officinalis Gaertn), bael (Aegle marmelos Corr.), Guava (, Psidium guajava), jamun or black plum (Syzygium cuminii L. Skeels.), Mango (Mangifera indica) and Papaya (Carica papaya).
In Vitro Plant Regeneration And Genetic Assessment Among Regenerates Using Molecular Markers In Aegle Marmelos Corr., Rajesh Pati
In Vitro Plant Regeneration And Genetic Assessment Among Regenerates Using Molecular Markers In Aegle Marmelos Corr., Rajesh Pati
Rajesh Pati
Bael (Aegle marmelos Corr.) is an important medicinal fruit tree. The fruit pulp contains marmelosin, which is a laxative, diuretic, is being used in many patented drugs in India. Micropropagation technology can be gainfully employed in mass multiplication of improved bael varieties. We have developed micropropagation protocol of bael through shoot bud culture. It was imperative to test genetic fidelity of micropropagated plants using molecular markers (RAPD, DAMD and ISSR).
Histological And Biochemical Changes In Aegle Marmelos Corr. Before And After Acclimatization, Rajesh Pati
Histological And Biochemical Changes In Aegle Marmelos Corr. Before And After Acclimatization, Rajesh Pati
Rajesh Pati
Maximum mortality of micropropagated plants occur during acclimatization phase because plantlets undergo rapid and extreme changes in physiological functioning, histological and biochemical changes. In order to investigate the actual reason of this limitation, test samples were collected at different stages of micropropagation of Aegle marmelos Corr. (In vitro stage, acclimation stage). The biochemical result showed that micropropagated plantlets produced significantly low total chlorophyll (0.042 mg/g fresh weight), reducing sugar (3.227%), NR activity (1.353 NO2/h/g fresh weight) and but higher protein (0.048 μg/g) during in vitro phase. The in vitro raised plants showed abnormal histological features like altered leaf mesophyll, absence …
Effect Of Agrobacterium Induced Necrosis, Antibiotic Induced Phytotoxicity And Other Factors In Successful Plant Transformation., Sandip S. Magdum
Effect Of Agrobacterium Induced Necrosis, Antibiotic Induced Phytotoxicity And Other Factors In Successful Plant Transformation., Sandip S. Magdum
Sandip S. Magdum
Agrobacterium tumefaciens infection and antibiotic wash are the critical steps of Agrobacterium mediated plant transformation procedure, most time responsible for lower transformation efficiency due to necrosis and phytotoxicity caused by biotic stress of Agrobacterium and abiotic stress by antibiotics respectively. Ammi majus Egyptian origin medicinal plant and Pearl millet cereal grain crop were studied for their stress responses to Agrobacterium mediated transformation (AMT). Agrobacterium strains LBA4404 (O.D.=0.6-0.8) and EHA105 (O.D.=0.2-0.4) were used for transformation experiments to infect calli of Ammi majus and embryogenic calli of Pearl millet respectively. Incase of antibiotic wash, Cefotaxime 500 mg L-1 was used for LBA4404 …
Antioxidant Potential, Cytotoxic Activity And Total Phenolic Content Of Alpinia Pahangensis Rhizomes, Halijah Ibrahim, Phang C. W., Abd Malek S. N.
Antioxidant Potential, Cytotoxic Activity And Total Phenolic Content Of Alpinia Pahangensis Rhizomes, Halijah Ibrahim, Phang C. W., Abd Malek S. N.
Halijah Ibrahim
BACKGROUND: Alpinia pahangensis, a wild ginger distributed in the lowlands of Pahang, Malaysia, is used by the locals to treat flatulence. In this study, the antioxidant and cytotoxic activities of the crude aqueous methanol and fractionated extracts of Alpinia pahangensis against five different cancer and one normal cell lines were investigated. The total phenolic content of each extract and its fractions were also quantified. This is the first report on the antioxidant and cytotoxic activities of Alpinia pahangensis extract. METHODS: In the current study, the crude methanol and fractionated extract of the rhizomes of Alpinia pahangensis were investigated for their …
Different Chemically Substituted Chitooligosaccharides Inhibit Beta-Secretase Activity, Rosna Binti Mat Taha
Different Chemically Substituted Chitooligosaccharides Inhibit Beta-Secretase Activity, Rosna Binti Mat Taha
Rosna Binti Mat Taha
Chitooligosaccharide or COS is a kind of oligosaccharide that is an integral part of the nervous system. COS is synthesized by chemical substitution and derived AE-COS, DEAE-COS and DMAE-COS that are being shown on IC(50) value. AE-COS and DMAE-COS exhibited four fold less inhibition than DEAE-COS. In addition, non-competitive inhibitor was identified via a Dixon plot and the Ki inhibition constant (100 mu g/mL). We declared, chemical substitution of COS is a water soluble human safe BACE-1 inhibitor.
Micropropagation Of Mangifera Indica L. Cv. Kurakkan Through Somatic Embryogenesis, Rajesh Pati
Micropropagation Of Mangifera Indica L. Cv. Kurakkan Through Somatic Embryogenesis, Rajesh Pati
Rajesh Pati
Nucellar embryogenesis was induced in Mangifera indica L. cv. Kurakkan, a polyembryonic salt tolerant, dwarfing rootstock. Nucellus tissue excised from 3.5 cm long fruits developed pro-embryonic callus in 19 days of inoculation on modified MS medium supplemented with 4.52μM 2,4- D, 0.05% malt extract and 13.78μM spermidine. Somatic embryogenesis exhibited high frequency (158.33 embryos). However, all the differentiated embryos proliferated on medium having low level of sucrose (4% w/v) and auxin (2.26μM 2,4-D). Most of the proembryonic calli converted into heart shaped and cotyledonary embryos by reducing temperature to 15oC. Somatic embryos were matured on modified MS medium fortified with …
Efficacy Of Different Detergents For Leaf Extract Based Sex Diagnostics Of Papaya (Carica Papaya L.)., Rajesh Pati
Efficacy Of Different Detergents For Leaf Extract Based Sex Diagnostics Of Papaya (Carica Papaya L.)., Rajesh Pati
Rajesh Pati
No abstract provided.
Antioxidant And Antibacterial Activities Of Ethanolic Extracts Of Asparagus Officinalis Cv. Mary Washington: Comparison Of In Vivo And In Vitro Grown Plant Bioactivities, Rosna Binti Mat Taha
Antioxidant And Antibacterial Activities Of Ethanolic Extracts Of Asparagus Officinalis Cv. Mary Washington: Comparison Of In Vivo And In Vitro Grown Plant Bioactivities, Rosna Binti Mat Taha
Rosna Binti Mat Taha
The antioxidant and antibacterial activities of ethanolic extracts of in vivo grown Asparagus officinalis cv. Mary Washington were investigated using superoxide dismutase, erythrocyte haemolysis and 2,2diphenyl-1-picrylhydrazil free radical scavenging methods. The measured antioxidant and antimicrobial potential were then compared to the activities offered by the ethanolic extracts of in vitro grown A. officinalis as well as ethanolic extract of undifferentiated callus cells of A. officinalis produced on Murashige and Skoog medium containing 1.5 mg/l 6-benzylaminopurine combined with 0.5 mg/l naphthalene acetic acid. The highest antioxidant capacity was obtained from the in vivo grown plant extract followed by in vitro grown …
The Apoptotic Effect Of 1 ' S-1 '-Acetoxychavicol Acetate From Alpinia Conchigera On Human Cancer Cells, Halijah Ibrahim, Aziz A. N., Nagoor N. H., Awang K., Azmi M. N., Aun L. I. L.
The Apoptotic Effect Of 1 ' S-1 '-Acetoxychavicol Acetate From Alpinia Conchigera On Human Cancer Cells, Halijah Ibrahim, Aziz A. N., Nagoor N. H., Awang K., Azmi M. N., Aun L. I. L.
Halijah Ibrahim
1'-(S)-1'-Acetoxychavicol acetate (ACA) isolated from the Malaysian ethno-medicinal plant Alpinia conchigera Griff. was investigated for its potential as an anticancer drug. In this communication, we describe the cytotoxic and apoptotic properties of ACA on five human tumour cell lines. Data from MTT cell viability assays indicated that ACA induced both time- and dose-dependent cytotoxicity on all tumour cell lines tested and had no adverse cytotoxic effects on normal cells. Total mortality of the entire tumour cell population was achieved within 30 hrs when treated with ACA at 40.0 microM concentration. Flow cytometric analysis for annexin-V and PI dual staining demonstrated …
Isolation, Purification And Biochemical Characterization Of Lectin From Oyster Mushroom, Pleurotus Sajor-Caju., Rajesh Pati
Isolation, Purification And Biochemical Characterization Of Lectin From Oyster Mushroom, Pleurotus Sajor-Caju., Rajesh Pati
Rajesh Pati
No abstract provided.
In Vitro Regeneration And Genetic Fidelity Testing Of Aegle Marmelos (Corr.) Plants, Rajesh Pati
In Vitro Regeneration And Genetic Fidelity Testing Of Aegle Marmelos (Corr.) Plants, Rajesh Pati
Rajesh Pati
No abstract provided.
In Vitro Plant Regeneration From Mature Explant Of Aegle Marmelos Corr. Cv. Cish-B2, Rajesh Pati
In Vitro Plant Regeneration From Mature Explant Of Aegle Marmelos Corr. Cv. Cish-B2, Rajesh Pati
Rajesh Pati
No abstract provided.
Micropropagation Of Guava (Psidium Guajava L.), Rajesh Pati
Micropropagation Of Guava (Psidium Guajava L.), Rajesh Pati
Rajesh Pati
A foolproof method of micropropagation through in vitro shoot bud culture has been developed in guava cv. ‘Allahabad Safeda’. The stock plant of guava was maintained at shade net house (75% shade) and pruned severely to encourage new growth. Around 3 cm long shoots were excised and defoliated. The explants were pretreated in solution containing 0.1% Carbendazime and 100 mg/L PVP for 1 hour and then washed with Tween-20 wetting agent. The pretreated explants were further treated with HgCl2 0.1% for 5 minutes aseptically followed by six washing in autoclaved distilled water. The sterilized explants were cultured on MS medium …
). Clonal Micropropagation Of Indian Gooseberry (Emblica Officinalis Gaertn)., Rajesh Pati
). Clonal Micropropagation Of Indian Gooseberry (Emblica Officinalis Gaertn)., Rajesh Pati
Rajesh Pati
No abstract provided.