Biotechnology Commons^™

Phytochemical Analysis And Safety Assessment Of Watercress Nasturtium Officinale: Antioxidant Capacity, Heavy Metal Profiling, And Toxicity Using Brine Shrimp Model, Qurrota A’Yunin, Chamini Kasun Hemachandra, Muhaimin Rifa’I, Yoga Dwi Jatmiko, Maftuch Maftuch, Rizky Fadilla Agustin Rangkuti, Cucun Herlina, Zulfa Mahdiatur Rasyida, Moh Dliyauddin

Karbala International Journal of Modern Science

Medicinal plants have attracted growing attention as sources of natural bioactive compounds for sustainable aquaculture and health. This study evaluated the phytochemical composition, antioxidant activity, heavy metal profile, and acute toxicity of watercress (Nasturtium officinale W.T. Aiton) methanol extract using maceration, qualitative and quantitative (TPC, TFC, antioxidant DPPH) phytochemical assay, XRF, and BSLA. Phytochemical screening of plant extracts showed the existence of flavonoids, alkaloids, phenols, tannins, triterpenoids, and saponins, but not steroidal. The TPC and TFC were 20.6 mg GAE/g and 10.8 mg QE/g, respectively. The extract displayed significant antioxidant activity (IC₅₀ = 64.78 µg/mL in DPPH assay). …

High-Throughput Robotic Ethanol Inhibition Assays For Engineered Thermophilic Biofuel Strains, Kevin He, Daniel Olson, Marybeth Maloney, Anthony Lanahan

Wetterhahn Science Symposium Posters

Ethanol stress assays are commonly used to evaluate microbial tolerance, metabolic adaptation, and fermentation performance. However, manual liquid handling introduces variability across replicate wells and small-volume pipetting steps, limiting reproducibility and throughput. This study developed an automated OT-2 robotic workflow to generate replicated ethanol concentration gradients for high-throughput inhibition assays in engineered thermophilic biofuel strains. Kinetic plate-reader measurements were used to quantify ethanol-dependent growth responses under anaerobic fermentation conditions. The reasearch question is: How do engineered thermophilic biofuel strains differ in ethanol-dependent growth inhibition under anaerobic fermentation conditions, and can automated robotic assays improve the reproducibility of these measurements? Can …

Interpretable Deep Learning Models For Trustworthy Prediction Of Enzyme Functions, Louis Dumontet

UNLV Theses, Dissertations, Professional Papers, and Capstones

Trustworthy prediction of enzyme function from protein sequences remains a central challenge in computational biology, particularly when annotated data are limited, imbalanced, or incomplete. This dissertation develops interpretable deep learning methods for enzyme discovery and enzyme function prediction from amino acid sequences. First, it introduces PEPIC, an interpretable convolutional neural network for substrate-level prediction of hydrolytic plastic-degrading enzymes. Using curated and expanded sequence datasets, PEPIC improved predictive performance over benchmark methods, identified sequence regions aligned with catalytic and substrate-binding residues, and supported the discovery and experimental validation of a previously uncharacterized PET-degrading enzyme. Second, this dissertation investigates the integration of …

Optimization Of Physalis Angulata L. Callus Induction And Salinity-Induced Antioxidant Production, Firstian Naufal Sistyananda, Erni Suminar, Anne Nuraini, Muhamad Kadapi, Murgayanti, Syariful Mubarok, Rahmat Budiarto, Eddy Renaldi, Kusumiyati

Jurnal Kultivasi

Groundcherry (Physalis angulata L.) is a plant with many medicinal potentials due to its rich secondary metabolites such as phenolic and flavonoid. However, conventional agriculture practices are still limited, especially in Indonesia. This study was divided into two stages. The first stage was conducted to determine optimal 6-BAP and 2,4-D combination for callus induction, while the second stage was conducted to determine callus phenolic, flavonoid, and antioxidant response to salinity stress. The first stage was arranged in factorial completely randomized design with two factors: 6-BAP (0, 2, and 4 mg/L) and 2,4-D (0, 0.5, 1, and 1.5 mg/L). The second …

Best Integrated Writing Spring 2026 - Complete Edition, Wright State University School Of Humanities And Cultural Studies, Tracy Smith, Kristie Mckiernan

Best Integrated Writing

Best Integrated Writing includes excellent student writing from Integrated Writing courses taught at Wright State University.

Hold Paramount The Health, Safety, And Welfare Of The Public And The Planet, Daniel B. Oerther

Civil, Architectural and Environmental Engineering Faculty Research & Creative Works

No abstract provided.

Geophysical Analysis Of Volcanic Soils Influencing Microbial Habitat Dynamics On Taal Volcano Island, Ray Albert E. La Rosa, Iluminada A. Ronio, Shaun Michael C. Basco, Martin Gabriel Z. Dela Cruz

The Lasallian Journal of Health

Taal Volcano Island in Batangas, Philippines, hosts an active post-caldera complex with a large hydrothermal system producing extreme soil environments. This study analyzed the geophysical and geochemical characteristics of shoreline soils to determine their potential as microbial habitats. Soil samples were collected at 14°02′04″N, 121°00′09″E, one meter below the surface of near newly formed vents. Physical parameters, including water content, bulk density, sedimentation, texture, and porosity, were measured alongside pH and temperature data. Results revealed sandy soil texture with 25% moisture, moderate compaction (1.66 g cm⁻³), high surface temperature (50 °C on-site; 72.7 °C PHIVOLCS data), and extreme acidity (pH …

Investigation Of Factors Influencing Electric Vehicle Adoption In Indonesia: Ev Owners’ Perspectives, Desrina Yusi Irawatia, Nur Aini Masruroh, Nur Mayke Eka Normasari

ASEAN Journal on Science and Technology for Development

Electric vehicle (EV) uptake in Indonesia remains markedly below policy benchmarks. This study applies the Unified Theory of Acceptance and Use of Technology version 3 (UTAUT3), an extension of UTAUT2 that incorporates personal innovativeness as an additional construct to examine its impact on both behavioral intention and actual EV adoption within the Indonesian context. Unlike studies that typically survey the general public, this study focuses on actual EV users and owners, providing more representative and responsive insights into real-world EV usage. A total of 208 respondents participated, with 135 from the Jabodetabek area and 73 from Surabaya. The UTAUT3 framework …

A Deep Neural Approach To Network-Based Obfuscated Malware, Meera Parmar, Sunil Gautam

ASEAN Journal on Science and Technology for Development

The rising prevalence of obfuscated malware poses a critical threat to network security, undermining traditional detection methods and jeopardizing data integrity and system reliability in an increasingly connected world. This growing danger highlights the urgent need for advanced solutions to protect against evolving cyber risks. This research intro-duces a novel framework to enhance malware detection, employing a hybrid architecture that integrates spatial and temporal analysis with attention mechanisms. The approach leverages a large dataset subsample, focusing on key feature selection and augmentation to improve robustness against evasion techniques. This innovative framework offers a significant advancement in identifying malicious network traffic, …

Extraction And Characterization Of Fresh And Dehydrated Cactus (Opuntia Ficus Indica) Polysaccharide For Hydrogel Preparation, Aye Thwe Thwesoe, May Myat Khine

ASEAN Journal on Science and Technology for Development

In this research, extraction of polysaccharide compounds from Cactus (Opuntia Ficus Indica) leaves in both fresh and dehydrated condition by solvent precipitation method using three types of water, acid (HCL) and NaOH. Before extraction, the physicochemical properties were examined to determine optimum yield (%) of extracted polysaccharide by optimization of Box-Behnken Design (BBD) of response surface methodology (RSM). The polysaccharide-based acrylamide hydrogel was prepared by free radical polymerization. The functional and structural characterization was done by FTIR, XRD and SEM for examination of extracted polysaccharide as raw polymer backbone in hydrogel preparation and prepared hydrogel as adsorbent for metal removal. …

Mechanical Property Evaluation Of Paper Die Materials In Explosive Forming, Higa Yoshikazu, Kadena Hisashi, Shimojima Ken, Iyama Hirofumi

ASEAN Journal on Science and Technology for Development

This study investigates the feasibility of using paper materials as substitutes for molds in explosive forming by systematically evaluating their mechanical properties. Tensile tests were conducted on three paper types differing in thickness and internal fiber structure, using a custom-designed gripping tool. Specimens were prepared in multiple orientations to account for anisotropic behavior. Results indicate that coated paper, exhibiting the highest specific tensile strength and energy absorption per unit basis weight, is the most suitable for formability. In contrast, thick paper, characterized by the lowest specific tensile energy absorption, offers the best dimensional accuracy. Anisotropy was observed in matte and …

Ai-Assisted Hybrid Ga–Pso Channel Allocation Under 3gpp Tr 38.901 Uma For Efficient 5g Radio Resource Management, Sharada Narsingrao Ohatkar

ASEAN Journal on Science and Technology for Development

The increased traffic and heterogeneity in the 5G/B5G network require efficient radio resource management (RRM). However, the existing methods, such as GA and PSO, have poor convergence speed and require proper initial conditions. Additionally, learning-based methods have high computational complexity. Hence, in this paper, a novel AI-assisted Hybrid Channel Allocation (AI–HCA) framework is proposed by using a support vector regression (SVR)-based predictive initialization method and GA-PSO optimization. Simulation results using the 3GPP UMa channel model show that the proposed method has a 28% reduction in call blocking probability (CBP), a 15-25% enhancement in spectral efficiency (SE), and a 18-25% enhancement …

Triangulating Primary Sources, Professional Judgement, And Llm-Generated Summaries: Educating Nurses In An Ai-First World, Sarah Oerther, Daniel B. Oerther

Civil, Architectural and Environmental Engineering Faculty Research & Creative Works

No abstract provided.

A Transdisciplinary Approach To Advancing Water Security And Public Health: The “Nurse + Engineer” Model For Achieving Sustainability In Wash Systems Using Community-Based Participatory Research, Daniel B. Oerther, Sarah Oerther

Civil, Architectural and Environmental Engineering Faculty Research & Creative Works

Human health is linked to the health of the environment, a concept referred to as planetary health. As environmental challenges become increasingly complex, single-disciplinary approaches are insufficient to achieve sustainable solutions. This article presents a transdisciplinary framework, termed the "Nurse + Engineer" model, as a novel methodology for addressing complex problems at the nexus of environmental engineering and public health. The model integrates the technical design and systems-level thinking of environmental engineering with the community-based, patient-centered care of nursing. This work details the key lessons learned from the retrospective application of this framework to a series of multi-year, community-based participatory …

Environmental Engineers Develop Solutions To Problems Of Planetary Health: The Legacy Of Professor P. Aarne Vesilind, Daniel B. Oerther

Civil, Architectural and Environmental Engineering Faculty Research & Creative Works

The principal ethical obligation of the engineering profession, to "hold paramount the health, safety, and welfare of the public," has been challenged for decades. Professor P. Aarne Vesilind identified two primary deficiencies: an anthropocentric bias that values nature only instrumentally and the use of non-mandatory, aspirational language for environmental protection, which resulted in the exclusion of enforceable environmental canons. This paper argues that two recent developments provide a comprehensive response to Vesilind's recommendations for engineering ethics. First, the United States Bureau of Labor Statistics has redefined environmental engineering as "developing solutions to problems of planetary health." Second, Oerther proposed a …

Overview And Methods Of Molecular Paleontology: A Review, Shelby Touchstone

Williams Honors College, Honors Research Projects

Molecular paleontology—the study of ancient biomolecules preserved in fossils and sediments, including ancient DNA, lipids, and proteins—has motivated researchers since the 1950s, inspiring both scientific inquiry and broader cultural imagination. Think Jurassic Park. This honors thesis presents a comprehensive review of the history, current state, and methods of the field of molecular paleontology. Drawing on peer-reviewed literature and scholarly resources accessed through databases such as EBSCO and Bierce library, this review examines key techniques such as Next Generation Sequencing and amino acid racemization and will go over limitations and controversies in the field. It will also explore potential new …

Design Of An Economic Order Quantity–Based Dashboard For Vaccine Packaging Inventory Management, Hadi Susanto, Susmitha Canny, Alexandra Elizabeth Callistha, Shakira Dwi Purwandari, Naila Davina Aurelia

ASEAN Journal on Science and Technology for Development

Background: Timely availability of vaccine packaging is critical to pharmaceutical supply chain reliability, as packaging materials play a key role in maintaining cold chain integrity during distribution. Inadequate availability of packaging materials can disrupt vaccine delivery even when finished vaccines are ready for shipment. The case company faces frequent delivery delays, insufficient safety stock, and irregular replenishment of vaccine packaging materials, which have jeopardized the organization’s target of achieving a 90% on-time delivery rate.

Objective: To design and evaluate an Economic Order Quantity (EOQ)–based inventory dashboard for vaccine packaging that standardizes ordering decisions and strengthens stock visibility in pharmaceutical distribution …

Labor Savings From Mechanical Rice Transplanting In Bangladesh, India, Nepal, And The Philippines: A Systematic Review And Meta-Analysis, Rodrigo S. David

ASEAN Journal on Science and Technology for Development

Rice production in South and Southeast Asia faces serious challenges, including acute labor shortages and rising wages, which threaten regional food security. Mechanical rice transplanting offers a promising solution; however, evidence on labor savings, especially region-specific data, remains inconsistent. This systematic review and meta-analysis aimed to quantify labor savings from mechanical versus manual transplanting across Bangladesh, India, Nepal, and the Philippines.

Following Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines, a systematic search was conducted across four major scientific databases (2000–2026) using Boolean search terms for mechanical transplanting and labor outcomes. Of 284 initial records, 52 met eligibility …

Climate And Seasonal Influences On Indo-Pacific Sea Level: Insights From 30 Years Of Satellite And Tide Gauge Data, Karlina Triana

ASEAN Journal on Science and Technology for Development

We analyze Indo-Pacific sea-level variability using monthly satellite altimetry (1993–2025) validated against records from 16 tide-gauge stations. Cross-comparisons show strong agreement, especially in the western Pacific, confirming the reliability of altimetry for regional assessments. Seasonal SLA variability is largest in the Bay of Bengal and South China Sea and reflects monsoonal forcing, whereas interannual fluctuations in the eastern Indian and western Pacific oceans are dominated by ENSO and modulated by PDO. Harmonic decomposition isolates annual and semi-annual cycles, and an EOF/PCA framework identifies the leading modes: EOF1 (30.8%) captures basin-scale interannual variability and EOF2 (20.9%) reflects the seasonal cycle. Spectral …

Evaluating The Role Of Power Electronics In Enhancing Renewable Energy And Smart Grid Performance: A Review, Muhammad Irfan Habib

ASEAN Journal on Science and Technology for Development

The review article demonstrates the importance of power electronics in propelling the increase of renewables and increasing the efficiency of contemporary electrical grids. In particular, it highlights the need for bidirectional converters for effective energy management in vehicle-to-grid (V2G) systems with two-way power transfer. The paper examines the series of complications offered by the significantly large integration of power electronic converter-interfaced renewables, such as those related to system steadiness, power quality, and resignation bottlenecks. Also, it recognizes a mix of new technologies, such as wide bandgap (WBG) semiconductors like silicon carbide (SiC) and gallium nitride (GaN), that deliver superior performance …

Artificial Neural Network Based Protection For Dc Bus Using Solid State Dc Breaker, Eswaraiah Giddalur, Askani Jaya Laxmi

ASEAN Journal on Science and Technology for Development

Low voltage Direct Current (LVDC) microgrids have been extensively used as a hopeful technology with high access to distributed energy resources (DERs) and DC loads, due to the absence of multiple conversions. A high amplitude Fault current together with the vulnerability of electronic devices, poses a great challenge to the line protection in LVDC microgrids. Electromechanical switches are slow and take long tripping time to clear the short circuit fault. Power electronic circuit breakers are used to achieve fast fault interruption recent progress in areas like machine learning and natural language processing have affected nearly every assiduity and area of …

Unlocking Green Potential In Indonesian Small Medium Enterprises: Challenges And Opportunities For Sustainable Vermicelli Production, Togar W. S. Panjaitan, Karina Agustin, Prayonne Adi, Jani Rahardjo, I Nyoman Sutapa

ASEAN Journal on Science and Technology for Development

Due to limited initiatives and support, the transition to green manufacturing among small and medium enterprises (SMEs) in developing countries remains challenging. Through a critical literature review and case study of the Indonesian vermicelli industry, this study highlights the importance of collaboration and strong policies to address this. Other findings also reveal that the energy intensity and emissions in the sector exceed best practice benchmarks, with the cooking and drying stages contributing significantly to emissions due to high energy consumption. Lack of government regulations, company awareness, and understanding of applicable practices and technologies creates challenges in green manufacturing. The study …

Hybrid Model For Phishing Website Detection Using Transfer Learning, Atul Dubal, Mansi Subhedar, Santosh Dhamala, Manasi Patil

ASEAN Journal on Science and Technology for Development

The rapid digitization of human activities has intensified reliance on internet-based platforms, creating fertile ground for cybercriminal exploits such as phishing. Despite advancements in detection mechanisms, phishing attacks continue to evolve, leveraging sophisticated visual mimicry to deceive users. This paper proposes a robust vision-based phishing detection system using ensemble deep learning to analyse webpage screenshots. The framework integrates transfer learning with pre-trained VGG16 and DenseNet121 models, extracting complementary low-level texture features (edges, gradients) and high-level hierarchical patterns (logos, layouts). These features are fused through a custom classifier with dropout regularization to mitigate overfitting. A balanced dataset of 3,000 webpage screenshots …

Bio-Orthogonal Chemistry-Based Strategy To Turn-On And Turn-Off Crispr-Cas9 Gene Editing In Solution And In Live Cells, Bhoomika Pandit

Electronic Theses & Dissertations (2024 - present)

The CRISPR–Cas9 system is a widely popular tool for genome engineering. There is a strong interest in developing tools for temporal control of CRISPR-Cas9 activity to address some of the challenges and to broaden the scope of potential applications. In this thesis I describe two biorthogonal based approaches to either Turn-ON and Turn-OFF CRISPR-Cas9 gene editing.

In first project work I describe a bio-orthogonal chemistry-based approach to Turn-ON Cas9 nuclease activity with temporal precision. We report a TCO-acylimidazole reagent that acylates 2′-OH groups of RNA. Poly-acylation (“cloaking”) of RNA was optimized in vitro using a model 18-nt oligonucleotide, as well …

Spilling The Tea: Effects Of Uv Disinfection On Bioactive Compounds In South African Rooibos Tea, Sora Ghanem, Leah Ankiah, Yusra Ahmed

Student Scholar Symposium

This study investigated the effects of ultraviolet (UV) light disinfection on key bioactive flavonoid compounds in rooibos tea (Aspalathus linearis). There is an interest in non-thermal food preservation technologies, and UV treatment offers potential advantages for beverage processing. However, concerns exist regarding possible degradation of health-promoting compounds during treatment. Rooibos tea samples were subjected to five UV exposure intensities (0, 25, 50, 75, and 100 mJ/cm²), and concentrations of four major flavonoids (aspalathin, orientin, vitexin, and rutin) were measured using high performance liquid chromatography (HPLC). Surprisingly, most flavonoids showed stability or slight concentration increases at higher UV doses. Aspalathin concentrations …

Environmental Risk Assessment Of Textile Waste From Bio-Engineered Fibers In The Fashion Industry, Aayush Pankaj Agarwal, Harshit Soral, Irmak Aktan, Karol Montserrat Gonzalez Saad, Maria Daniela Saa Espinosa, Sheetal Suhas Dhanuka, Yi Yang

Harrisburg University Dissertations and Theses

The global fashion industry generates over 92 million tons of textile waste annually, a figure projected to increase significantly by 2030. Bio-engineered fibers, including bacterial cellulose, mycelium-based leather, and recombinant protein textiles emerge as sustainable alternatives to conventional fibers. Despite their promise, recent evidence suggests that these fibers may pose environmental risks at the end-of-life stage, such as incomplete biodegradation in landfills, microfiber shedding, and chemical leaching from dyes and coatings. The purpose of this study is to examine how these risks are represented through scientific literature, corporate sustainability reports, and policy frameworks, and to identify strategies for integrating risk …

Generating Predictive Gene Expression Signatures For Alzheimer's Disease Using Postmortem Brain Tissue, Ashley Duche

Pharmaceutical Sciences (PhD) Dissertations

Background: Alzheimer’s Disease (AD) is a progressive neurodegenerative disorder characterized by the accumulation of amyloid-beta (Aβ) plaques and tau protein aggregates. These pathological features develop in specific brain regions, but why some areas are more vulnerable to early AD-related changes remains unclear. To address this, predictive gene expression signatures were developed to explore the molecular mechanisms underlying regional susceptibility to AD pathology.

Methods: This was performed using postmortem brain (PMB) tissue from participants in the Religious Orders Study and Memory and Aging Project (ROSMAP), Mayo Clinic, and Mount Sinai Brain Bank (MSBB) to generate gene expression signatures from six brain …

Synthesis Of Antibody Functionalized Polyaniline/Polystyrene/N-Gqds Composite Fibermats For Sweat Based Cortisol Biosensing Applications, Ashwin James

Honors Theses

Noninvasive, wearable biosensors capable of detecting stress biomarkers in sweat require electrodes that are flexible, conductive, insoluble in water, and highly sensitive at low concentrations of analyte. In this work, bovine serum albumin blocked and anti-cmab immobilized nitrogen doped graphene quantum dots integrated polyaniline/polystyrene composite fibermat electrodes (BSA/Anti-Cmab/N-GQDs/PANI/PS electrodes) were synthesized and evaluated as a potential platform for an electrochemical cortisol biosensor in sweat based systems. Polyaniline was utilized in order to provide electrical conductivity, while polystyrene served as a carrying polymer for mechanical support and its hydrophobicity. Composite fibermats were manufactured through Forcespinning™, followed by a secondary polyaniline graft …

Low-Cost Cutaneous Protoporphyrin Ix (Ppix) Detection (Cpd) Device For Follow-Up Monitoring Of Patients After Photodynamic Therapy, Md Asaduzzaman Rasel

Graduate Masters Theses

Background: Photodynamic Therapy (PDT) utilizes specific wavelengths of light to activate photosensitizing chemical compounds, known as photosensitizers, which induce the generation of cytotoxic reactive oxygen species (ROS) for the targeted destruction of cancer cells. Among various photosensitizers for PDT, Protoporphyrin IX (PpIX) is widely employed in oncology and dermatology due to its natural in situ generation via the metabolic conversion of 5- aminolevulinic acid (ALA), a non-phototoxic prodrug. Systemic administration of ALA after 3-6 hr drug delay leads to peak PpIX accumulation in tissues, facilitating therapeutic and diagnostic applications. However, PpIX can persist in the skin for 24–48 hours post-treatment, …

Volumetric Imaging Of Cellular Dynamics Using Light-Sheet Microscopy: From Subcellular Structures To Whole Organisms, Md Nasful Huda Prince

Optical Science and Engineering ETDs

Advancing biomedical imaging requires platforms that combine high-resolution, speed, and stability across biological scales. This dissertation presents three innovations designed to address limitations of conventional light-sheet and oblique plane imaging. First, we introduce Reflected Inline Detection in Epi-Oblique Plane Microscopy (RIDE-OPM), a compact and drift-resistant system that eliminates a tertiary detection path while maintaining alignment flexibility, enabling robust long-term live imaging. Second, we present a high-speed Axially Swept Light-Sheet Microscopy (ASLM) platform for cleared tissues, using dual foci and synchronized sweeping to achieve isotropic sub-micron resolution at up to 40 frames-per-second, a fourfold improvement over standard ASLM. Integrated with a …