Open Access. Powered by Scholars. Published by Universities.®
- Keyword
-
- 3. MEDICAL AND HEALTH SCIENCES (1)
- 3.3 HEALTH SCIENCES (1)
- 3.4 HEALTH BIOTECHNOLOGY (1)
- Article (1)
- Biochemistry and molecular biology (1)
-
- Bioinformatics (1)
- Biology (1)
- Biomaterials (1)
- Cell biology, (1)
- Codon optimization (1)
- Department of biological sciences (1)
- Epidemiology (1)
- Expression host (1)
- Infectious diseases (1)
- Johne's disease (1)
- MAP antigens (1)
- MAP vaccine (1)
- MMP (1)
- MptD (1)
- Paratuberculosis (1)
- Pdf (1)
- Pharrnacogenomics (1)
- Preterm Neonates (1)
- Public and environmental health (1)
- Sepsis (1)
- Staphylococcus epidermidis Clones (1)
- Virology (1)
Articles 1 - 2 of 2
Full-Text Articles in Biotechnology
Draft Genome Sequences Of Six Different Staphylococcus Epidermidis Clones, Isolated Individually From Preterm Neonates Presenting With Sepsis At Edinburgh's Royal Infirmary, Paul Walsh, M. Bekaert, J. Carroll, T. Manning, B. Kelly, A. O'Driscoll, X. Lu, C. Smith, P. Dickinson, K. Templeton, P. Ghazal, Roy D. Sleator
Draft Genome Sequences Of Six Different Staphylococcus Epidermidis Clones, Isolated Individually From Preterm Neonates Presenting With Sepsis At Edinburgh's Royal Infirmary, Paul Walsh, M. Bekaert, J. Carroll, T. Manning, B. Kelly, A. O'Driscoll, X. Lu, C. Smith, P. Dickinson, K. Templeton, P. Ghazal, Roy D. Sleator
Department of Biological Sciences Publications
Herein, we report the draft genome sequences of six individual Staphylococcus epidermidis clones, cultivated from blood taken from different preterm neonatal sepsis patients at the Royal Infirmary, Edinburgh, Scotland, United Kingdom.
Enhanced Expression Of Codon Optimized Mycobacterium Avium Subsp. Paratuberculosis Antigens In Lactobacillus Salivarius, Christopher D. Johnston, John P. Bannatine, Rodney Govender, Lorraine Endersen, Daniel Pletzer, Helge Weingart, Aidan Coffey, Jim O'Mahony, Roy D. Sleator
Enhanced Expression Of Codon Optimized Mycobacterium Avium Subsp. Paratuberculosis Antigens In Lactobacillus Salivarius, Christopher D. Johnston, John P. Bannatine, Rodney Govender, Lorraine Endersen, Daniel Pletzer, Helge Weingart, Aidan Coffey, Jim O'Mahony, Roy D. Sleator
Department of Biological Sciences Publications
It is well documented that open reading frames containing high GC content show poor expression in A+T rich hosts. Specifically, G+C-rich codon usage is a limiting factor in heterologous expression of Mycobacterium avium subsp. paratuberculosis (MAP) proteins using Lactobacillus salivarius. However, re-engineering opening reading frames through synonymous substitutions can offset codon bias and greatly enhance MAP protein production in this host. In this report, we demonstrate that codon-usage manipulation of MAP2121c can enhance the heterologous expression of the major membrane protein (MMP), analogous to the form in which it is produced natively by MAP bacilli. When heterologously over-expressed, antigenic determinants …