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Full-Text Articles in Life Sciences

Two Decades Of Warming Increases Diversity Of A Potentially Lignolytic Bacterial Community, Grace Pold, Jerry M. Melillo, Kristen Deangelis Jan 2015

Two Decades Of Warming Increases Diversity Of A Potentially Lignolytic Bacterial Community, Grace Pold, Jerry M. Melillo, Kristen Deangelis

Microbiology Department Faculty Publication Series

As Earth's climate warms, the massive stores of carbon found in soil are predicted to become depleted, and leave behind a smaller carbon pool that is less accessible to microbes. At a long-term forest soil-warming experiment in central Massachusetts, soil respiration and bacterial diversity have increased, while fungal biomass and microbially-accessible soil carbon have decreased. Here, we evaluate how warming has affected the microbial community's capability to degrade chemically-complex soil carbon using lignin-amended BioSep beads. We profiled the bacterial and fungal communities using PCR-based methods and completed extracellular enzyme assays as a proxy for potential community function. We found that …


Long-Term Forest Soil Warming Alters Microbial Communities In Temperate Forest Soils, Kristen Deangelis, Grace Pold, Begüm D. Topçuoğlu, Linda T.A. Van Diepen, Rebecca M. Varney, Jeffrey L. Blanchard, Jerry Melillo, Serita D. Frey Jan 2015

Long-Term Forest Soil Warming Alters Microbial Communities In Temperate Forest Soils, Kristen Deangelis, Grace Pold, Begüm D. Topçuoğlu, Linda T.A. Van Diepen, Rebecca M. Varney, Jeffrey L. Blanchard, Jerry Melillo, Serita D. Frey

Microbiology Department Faculty Publication Series

Soil microbes are major drivers of soil carbon cycling, yet we lack an understanding of how climate warming will affect microbial communities. Three ongoing field studies at the Harvard Forest Long-term Ecological Research (LTER) site (Petersham, MA) have warmed soils 5°C above ambient temperatures for 5, 8, and 20 years. We used this chronosequence to test the hypothesis that soil microbial communities have changed in response to chronic warming. Bacterial community composition was studied using Illumina sequencing of the 16S ribosomal RNA gene, and bacterial and fungal abundance were assessed using quantitative PCR. Only the 20-year warmed site exhibited significant …


Syllabus: Sustainable Living: Solutions For The 21st Century, Lena Fletcher Jan 2015

Syllabus: Sustainable Living: Solutions For The 21st Century, Lena Fletcher

Sustainability Education Resources

In this innovative interdisciplinary course you will work with your peers to research and understand how sustainability in different contexts presents solutions to many problems facing modern society. You will work in teams to investigate, evaluate, communicate, and reflect on the multifaceted challenges associated with natural resource use, food systems, energy, transportation, waste, the built environment, water quality, and climate change. You will also research case studies, debate controversies, assess political and cultural contexts, investigate technological advances, and identify gaps in scientific knowledge. Using these resources, you and your peers will be tasked with developing your own sustainable solutions for …


Syllabus: Sustainable Living: Solutions For The 21st Century, Laurie Simmons Jan 2015

Syllabus: Sustainable Living: Solutions For The 21st Century, Laurie Simmons

Sustainability Education Resources

Your generation has been handed an opportunity: the opportunity to change the story of our culture from one of blind consumption to one of conscious ecological living. As individuals, as a society and as a species, we are facing challenges related to sustainability including economic stability, social justice, resource depletion and waste management, biodiversity preservation, climate adaptation and food and agricultural resilience. In this interdisciplinary course you will learn how to seize this opportunity by working with your peers to research and understand how sustainability in different contexts presents solutions to many of these challenges facing modern society. “Sustainable Living” …