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Full-Text Articles in Life Sciences
On Computing Relative Effective Population Size Estimates And Parameters From An Equilibrium Cycle Of Hermaphrodite Frequency Fluctuation Due To Mixed Reproductive Modes In Filamentous Fungi, Christopher Toomajian
On Computing Relative Effective Population Size Estimates And Parameters From An Equilibrium Cycle Of Hermaphrodite Frequency Fluctuation Due To Mixed Reproductive Modes In Filamentous Fungi, Christopher Toomajian
Fungal Genetics Reports
Many filamentous ascomycete fungi reproduce primarily asexually, with only occasional sexual generations. This can lead to a departure from the 1:1 mating type ratio that is expected in obligate sexual populations. The relaxed selection on sexual traits also can lead to a decrease in the frequency of female fertile strains in field populations, while male fertility does not similarly decrease since male gametes also can serve as asexual spores. Both changes ultimately impact the strength of genetic drift in populations. The frequency of female sterility likely increases with the time since the last generation of sexual reproduction, such that it …
Relative Efficiency Of Split-Marker Versus Double-Crossover Replacement Protocols For Production Of Deletion Mutants In Strain Ph-1 Of Fusarium Graminearum, Sladana Bec, Gabdiel E. Yulfo-Soto, Lisa J. Vaillancourt
Relative Efficiency Of Split-Marker Versus Double-Crossover Replacement Protocols For Production Of Deletion Mutants In Strain Ph-1 Of Fusarium Graminearum, Sladana Bec, Gabdiel E. Yulfo-Soto, Lisa J. Vaillancourt
Fungal Genetics Reports
The split-marker (SM) protocol has become a popular method for production of knockout mutations in fungi. We used Southern hybridization to compare the performance and efficiency of the SM protocol with the more traditional double-crossover intact marker (IM) method for creating deletions of the mating type genes in Fusarium graminearum. Both methods successfully produced knockouts at a rate of between 24 and 75%: the SM method produced mutants more efficiently for larger constructs (>1 kb), but it was similar to IM for a smaller construct that was 865 bp. Both methods also produced strains with additional ectopic integrations …
Neurospora 2021 - Program And Abstracts, Neurospora Conference
Neurospora 2021 - Program And Abstracts, Neurospora Conference
Fungal Genetics Reports
Neurospora 2021 - Program and Abstracts