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Changsha University of Science and Technology
Ampelopsis grossedentata; polysaccharide; ultrasonic; cellulase; pecyinase
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Research On Extraction Technology Of Ampelopsis Grossedentata Polysaccharide By Ultrasound-Assisted Enzymatic Method, Wang Xiao-Hui, Yao Mao-Jun, Chen Yi-Jun, Zhu Lu-Yi, Huang Xiang-Yu
Research On Extraction Technology Of Ampelopsis Grossedentata Polysaccharide By Ultrasound-Assisted Enzymatic Method, Wang Xiao-Hui, Yao Mao-Jun, Chen Yi-Jun, Zhu Lu-Yi, Huang Xiang-Yu
Food and Machinery
Objiective: In order to improve the extraction efficiency of polysaccharide from Ampelopsis grossedentata. Methods: Ultrasound-assisted enzymatic extraction method was used in this study to optimize the extraction process of polysaccharide from Ampelopsis grossedentata. The effects of ultrasound temperature, ultrasound extraction time, enzymolysis pH, ultrasound power and the addition of complex enzyme on the extraction efficiency of polysaccharide from Ampelopsis grossedentata were investigated by single factor test. And the significant factors, including enzymolysis pH, ultrasonic power and he addition of complex enzyme were by Plackett-Burman test. The optimal extraction conditions were obtained through the steepest climbing test and Box-Behnken test. Results: …