Life Sciences Commons^™

Innovative Creation Of A Laboratory Exercise: Fluorescent Staining Of Primary Chicken Embryo Fibroblast Culture Using Mitotracker And Hoechst Stains., Dwight Ginn

Scholar Week 2016 - present

Primary chicken embryo fibroblast cell cultures have been grown in the Developmental Biology course laboratory for many years. With the acquisition of a fluorescent microscope in the Biological Sciences department, it was possible to create a new laboratory exercise incorporating fluorescent staining of these cells. During the fall 2016 semester, primary chicken embryo fibroblast cell cultures were generated from 14 day old chicken embryos, then grown in EMEM with 10% FBS. Living cells were first treated with MitoTracker Orange CMTMRos that stains the mitochondria fluorescent orange. Cells were fixed and permeabilized, then counterstained with Hoechst 33342 DNA stain which stains …

Using Fluorescence Microscopy To Identify A Potential New Treatment For Heart Failure, Ryan D. Himes

Scholar Week 2016 - present

Cardiac glycosides have been used to treat heart failure for centuries, but they have a narrow therapeutic window, as they inhibit their target receptor nearly irreversibly. Overdoses can lead to arrhythmias. Phospholemman is a natural inhibitor of the same target as cardiac glycosides. It is possible that mutating phospholemman could achieve the same therapeutic benefit, while allowing cells to reverse the inhibition and thereby avoid an arrhythmia. I used fluorescence microscopy to screen candidate phospholemman mutants and identify one that binds more avidly to its target than the naturally occurring phospholemman. This mutant, L30A, caused similar effects as cardiac glycosides …

Illinois & Indiana Eco-Sites & Dnr Programs In The Watershed, Bob Caveny

Kankakee River Watershed Conference

Author Keywords:

Invasives control, IDNR, Private lands, Watersheds

Agricultural Practices To Improve Water Quality, Jeff O'Connor, Lauren Lurkins

Kankakee River Watershed Conference

Author Keywords:

Nutrient Loss Reduction Strategy, Illinois Farm Bureau programs, BMPs

Sand Trends And Habitat Degradation, Jerrod Parker, Drew Costenbader, Yong Cao, John Epifanio

Kankakee River Watershed Conference

Scholarship domains: Discovery, Teaching and Learning

Author keywords: Sand Movement, Fish Diversity, Kankakee River, Sportfish, Side-scan sonar

Fisheries & Stream Quality Update For The Kankakee River Basin, Stephen Pescitelli

Kankakee River Watershed Conference

Author keywords: stream quality, index of biotic integrity, walleye stocking

Scholarship domain: community application

Welcome, Randal D. Johnson

Kankakee River Watershed Conference

Remarks from conference planner, Dr. Randy Johnson.

Conference Planning Committee:

• Dr. Randal Johnson, Olivet Nazarene University
• J.R. Black, IL KRBC & NIAA
• Kevin Culver, Aqua
• Chad Miller, IL Farm Bureau
• Lynn Boerman, IL DNR
• Dr. Mike Pyle, Olivet Nazarene University
• Jennifer Love, Olivet Nazarene University