Life Sciences Commons^™

Site Directed Mutagensis Of Bacteriophage Hk639 And Identification Of Its Integration Site, Madhuri Jonnalagadda

Masters Theses & Specialist Projects

Bacteriophages affect bacterial evolution, pathogenesis and global nutrient cycling. They are also the most numerous and diverse group of biological entities on the planet [1, 2, 3, 4, 5, 6]. Members of the Lambda phage family share a similar genetic organization and control early gene expression by suppressing transcription, a process known as antitermination. Transcription antitermination in Lambda is mediated by a phage-encoded protein whereas in lambdoid phage HK022, antitermination is mediated by a phage-encoded RNA molecules. Recent results suggest that another bacteriophage called HK639 also appears to use RNA-mediated antitermination. To characterize this newly identified phage we generated site …

Towards An Action Spectrum For Photoentrainment Of The Chlamydomonas Reinhardtii Circadian Clock, Christa Gaskill

Masters Theses & Specialist Projects

No abstract provided.

An Analysis Of Illegal Bushmeat Availability In Local Restaurants Located In Voi, Kenya, Bridget A. Sutton

Masters Theses & Specialist Projects

The illegal bushmeat harvest has been identified as a reason for declining wildlife populations throughout much of Africa. For many years the trade was thought to exist primarily in Western Africa. The illegal use of bushmeat in Eastern Africa, including Kenya, went undocumented and unstudied. In 2004, the Born Free Foundation published a study which claimed illegal sale of wild game was substantial in butcheries throughout Nairobi, Kenya. In an effort to determine other markets of the commercial trade, the goal of this study was to analyze local restaurants in Voi, Kenya for illegal bushmeat sale. The town of Voi …

Mathematical Models Of Zea Mays: Grain Yield And Aboveground Biomass Applied To Ear Flex And Within Row Spacing Variability, Todd Curtis Ballard

Masters Theses & Specialist Projects

Field studies were conducted during the summers of 2007 and 2008 at the Agricultural Research and Education Complex, Western Kentucky University, Warren County, KY and commercial production fields in Caldwell County, KY, Warrick County IN, and Vanderburgh County, IN. The goals of these studies were to further validate the Duncan grain yield model, the Russell aboveground biomass model, and to study the effect of inconsistent spacing within rows on Zea mays L. yield. Plant spacing other than uniform decreases grain yield and profitability. The population experiments conducted at the Warren County location were a randomized complete block design with three …

Kentucky Warbler (Vol. 84, No. 4), Kentucky Library Research Collections

Kentucky Warbler

No abstract provided.

Alfred Russel Wallace, Journalist, Charles H. Smith

DLPS Faculty Publications

No abstract provided.

Manning, Allen, 1864-1950 (Sc 1762), Manuscripts & Folklife Archives

MSS Finding Aids

Finding aid only for Manuscripts Small Collection 1762. Journal of farm work done by Allen Manning and his co-workers for Mrs. Underwood of Warren County, Kentucky. He makes note of the weather and its effect on the crops.

Ford, Marion Conner, 1888-1940 (Sc 1720), Manuscripts & Folklife Archives

MSS Finding Aids

Finding aid only for Manuscripts Small Collection 1720. Correspondence related to Marion Conner Ford's position as director of the Ogden Department of Science at Western Kenktucky State Teachers College and his involvement with the College's farms.

Warren County, Kentucky Garden Club - Scrapbook, 1933-1956 (Sc 1740), Manuscripts & Folklife Archives

MSS Finding Aids

Finding aid only for Manuscripts Small Collection 1740. Scrapbook compiled by Ethel Alma Folllin containing minutes, news clippings, programs, etc. related to the activities of the Warren County Garden Club and its members.

Kentucky Warbler (Vol. 84, No. 3), Kentucky Library Research Collections

Kentucky Warbler

No abstract provided.

Conversion Of Traditional Observation-Based Botany Labs To Investigative Inquiry Learning, Hajara Mahmood

Masters Theses & Specialist Projects

“Tell me and I forget, show me and I remember, involve me and I understand.” - Chinese Proverb. Involvement in learning implies possessing skills and attitudes that permit students to seek resolutions to questions and issues while constructing new knowledge.

Low enrollment in Plant Biology and Diversity and upper level plant science courses has been noticed at Western Kentucky University. In addition, graduating students performed below the national average on the senior assessment examination in the area of botany content knowledge offered by WKU’s Biology Department. This may be due to the fact that observation-based botany has been taught in …

Genetic Diversity In Native And Invasive Rubus (Rosaceae), Ashley A. Wint

Masters Theses & Specialist Projects

Invasive species are an increasing threat to biological diversity as well as a leading cause of recent species’ extinctions. Invasives spread quickly and efficiently, and the U.S spends millions of dollars annually in the control and eradication of these species. More information is necessary in order to predict which species may become invasive. Rubus (Rosaceae) was chosen for study because this genus includes various ploidy levels, reproductive modes, and species that are invasive as well as native.

Three Rubus species were chosen to represent apomictic and tetraploid invasives (Rubus armeniacus), a sexual and diploid native species (R. occidentalis), and a …

Trypanosoma Cruzi In Wild Raccoons And Opossums From Kentucky, Brian Chad Groce

Masters Theses & Specialist Projects

Only 6 autochthonous cases of human Chagas disease have been documented in the U.S.A., however, as many as 5% of immigrants from Latin America may be infected with the etiologic agent, Trypanosoma cruzi. The parasite has been isolated from a variety of wild mammals, particularly in the southeastern region of the U.S.A. The goal of our study was to determine if the sylvatic cycle of T. cruzi infection occurs in Kentucky, and, if present, to assess the prevalence of infection in Warren and Barren counties. Raccoons and opossums were live-trapped between June and December, 2007. Animals were anesthetized with …

At Least Four Distinct Circadian Regulatory Mechanisms Required For All Phases Of Rhythms In Mrna Amount, Sigrid Jacobshagen, Bruce Kessler, Claire Rinehart

Mathematics Faculty Publications

Since the advent of techniques to investigate gene expression on a large scale, numerous circadian rhythms in mRNA abundance have been reported. These rhythms generally differ in amplitude and phase. First studies on circadian rhythms of transcription on a large scale are also emerging. We investigated to what extent the same circadian regulatory mechanism of transcription can give rise to rhythms in RNA amount that differ in phase solely based on a parameter that is not regulated by the circadian clock. Using a discrete-time approach, we modeled a sinusoidal rhythm in transcription with various constant exponential RNA decay rates. We …

Winstead, Joe Everett, 1938-2019 - Collector (Sc 1690), Manuscripts & Folklife Archives

MSS Finding Aids

Finding aid only for Manuscripts Small Collection 1690. Correspondence between Western Kentucky University students and members of Kentucky's congressional delegation related to air pollution and its potential effects on the state's agricultural economy.

Stamps, Rosalyn Marie (Gourley), 1919-2008 - Collector (Mss 223), Manuscripts & Folklife Archives

MSS Finding Aids

Finding aid only for Manuscripts Collection 223. Three recipe books collected by Rosalyn Marie (Gourley) Stamps; eight yearbooks, correspondence, and miscellaneous items from the Mabel Thomas Garden Club, Bowling Green, Kentucky.

Price, Sarah Frances "Sadie," 1849-1903 (Mss 212), Manuscripts & Folklife Archives

MSS Finding Aids

Finding aid only for Manuscripts Collection 212. Journal articles, scrapbook and botanical illustrations of Sarah Frances "Sadie" Price, a Bowling Green, Kentucky naturalist and artist. Also includes a copy of her book "Flora of Warren County, Kentucky" and a botanical card game, "Phaenogamia," that she developed.

Owsley, Rebecca (Fa 258), Manuscripts & Folklife Archives

FA Finding Aids

Finding aid and full-text scan of paper (Click on “Additional Files” below) for Folklife Archives Project 258. Paper: "Farm Folklore" written by Rebecca Owsley for a Western Kentucky University folk studies class.

Independent Strawberry Growers' Association - Bowling Green, Kentucky (Sc 1640), Manuscripts & Folklife Archives

MSS Finding Aids

Finding aid only for Manuscripts Small Collection 1640. Minutes, membership lists, financial reports and sundry forms from the Independent Strawberry Growers Association, Bowling Green, Kentucky. Includes picking card with agreement to pick for season on back. Two related photographs from the Kentucky Library & Museum Collection are included as additional files.

Drake, James F. (Sc 1644), Manuscripts & Folklife Archives

MSS Finding Aids

Finding aid only for Manuscripts Small Collection 1644. Letter from James F. Drake, Lexington, Kentucky to John Allen Gano, Centerville, Bourbon County, Kentucky, inquiring about a milk cow he has advertised for sale.

Kentucky Warbler (Vol. 84, No. 2), Kentucky Library Research Collections

Kentucky Warbler

No abstract provided.

Landscape Of The Past: The 1815 Log House At Western Kentucky University, Elizabeth Alewine

Masters Theses & Specialist Projects

The 1815 Log House is located on the campus of Western Kentucky University. Built in the early 1800's by Archibald Felts, the house was occupied by his descendants until 1968. The dogtrot floor plan, V-notched logs, and stone chimneys are some of the historical architectural features that can be viewed. It was donated to the Kentucky Library & Museum at WKU in 1980, and now serves as an on-site exhibit of early frontier life in Kentucky. The new landscape design for the log house includes a kitchen garden with period-appropriate plants and outdoor demonstration areas. The inventories and journals of …

Effects Of Exercise Induced Fatigue On Postural Balance: A Comparison Of Treadmill Versus Cycle Fatiguing Protocols, Katherine Wright

Masters Theses & Specialist Projects

The purpose of this study was to examine the effects of muscle fatigue after a treadmill versus cycle ergometer incremental test on postural balance indices and recovery time recreationally trained individuals. The Bruce Treadmill Test and the Incremental Cycle Ergometer test were used to induce fatigue. Each subject (N=16) performed both exercises, but the order of the exercises was randomized. The subjects performed balance measures on a Biodex Balance System via the Dynamic Balance Test at level 5 and indices were recorded as given. Balance was measured a total of nine times (pre-exercise, immediately post, and at 3, 6, 9, …

South Central Kentucky Audubon Society (Mss 205), Manuscripts & Folklife Archives

MSS Finding Aids

Papers, newsletters, minutes and yearbooks of the South Central Kentucky Audubon Society. Also includes correspondence related to the establishment of the Society in Bowling Green, Kentucky.

Searching For Binding Partners For The Novel Phkg1 Variant, Phkγ 181, Kishore Polireddy

Student Research Conference Select Presentations

Phopshorylase kinase (PhK) is a hexadecameric holoenzyme made up of four different subunits in the arrangement (αβγδ)4 and has total molecular mass of 1.3MDa. Alpha and β are regulatory subunits, γ is catalytic, and δ is an intrinsic molecule of calmodulin. PhK is a serine threonine kinase with glycogenolytic regulatory functions. Our lab has recently discovered that the γ subunit can be alternatively processed to produce a truncated form of 181 residues (γ-181). This variant of γ contains a phosphorylation site for PK-C, and its activity is influenced by this phosphorylation. We are using a LexA based yeast two hybrid …

38th Annual Wku Student Research Conference, Student Research Council, Western Kentucky University

Student Research Conference Select Presentations

Western Kentucky University 38th Annual Student Research Conference program and student abstracts. Saturday, April 12, 2008, Carroll Knicely Conference Center, Bowling Green, Kentucky.

'Hussel,' 'Bussel' And 'Kussel,' Or, Using Google Books To Stalk The Elusive Alfred Russel Wallace, Charles H. Smith

DLPS Faculty Publications

No abstract provided.

Biomechanical Modeling Of Forelimb Adduction In The Eastern Mole, Scalopusaquaticus, Mark Sandefur

Mahurin Honors College Capstone Experience/Thesis Projects

The hypertrophied musculoskeletal system driving adduction of Sealopus aquatieus's forelimb supports the locomotion of these burrowing subterranean mammals. Due to the excessive bulk of the major muscles contributing to this action, it was thought that this particular species may represent a rare case of a mammalian species with a greatly enhanced force contribution from each muscle, correlating with a reduction in the potential for the forelimb to adduct at a high velocity. By assessing the lever ratios of five significant muscles contributing to forelimb adduction, combined with vector analyses for the angles of these muscles' insertions, a model was established …

Development And Recycling Of Novel Arsenic Removal Technology, Morgan Jones

Mahurin Honors College Capstone Experience/Thesis Projects

As of 2006, the U.S. Environmental Protection Agency (EPA) changed the Maximum Contaminant Level (MCL) for arsenic from 50 parts per billion (ppb), to 10ppb because of links to cancer. Current remediation technologies are expensive; therefore, this change will result in increased economic pressure on rural communities with high levels of arsenic in their drinking water. Lowering of the standard has spurred the development of a novel remediation technology that has shown the ability to reduce arsenic in drinking water at the source, with the added benefit of low-cost disposal of a stable and benign waste product in ordinary landfills. …

Reminiscences: Impact Of Textiles And Apparel Technology On Our Lives, Ann Beth Presley, Martha C. Jenkins

Applied Human Sciences

Women's perceptions of technological changes in textiles and apparel and how the changes have affected daily life were examined using the transmissive reminiscence technique. Technological advances do contribute to use of less time and effort and offer a greater variety of products, but the products often have a shorter life expectancy or are kept in inventory for a shorter time because it is more expedient to replace rather than repair or recycle textile and clothing items. Care must be taken to cultivate cohesive relationships within the family and teach values that were once inherent in textiles/ apparel tasks. Otherwise advances …