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Molecular Cloning Of An \Alpha-Amylase Gene From Bacillus Subtilis Rskk246 And Its Expression In Escherichia Coli And In Bacillus Subtilis, Numan Özcan, Adem Altinalan, Mehmet Sai̇t Eki̇nci̇
Molecular Cloning Of An \Alpha-Amylase Gene From Bacillus Subtilis Rskk246 And Its Expression In Escherichia Coli And In Bacillus Subtilis, Numan Özcan, Adem Altinalan, Mehmet Sai̇t Eki̇nci̇
Turkish Journal of Veterinary & Animal Sciences
Bacillus subtilis RSKK246 was found to produce approximately a 65-kDa \alpha-amylase enzyme. A gene was isolated encoding \alpha-amylase activity that corresponded to this size and was inserted into pUC18 plasmid which was transferred to Escherichia coli. An approximately 1.7kbp fragment, which contains a whole \alpha-amylase gene, was excised and inserted into pUB110 and then transferred into the different B. subtilis strains including RSKK246, RSKK243, RSKK244, YB886 and ORBAM. The a-amylase gene was cloned into the plasmids and expressed with its own promoter, and this promoter sequence seemed to function in the E. coli and in all B. subtilis strains. Specific …
Cloning Of Bacillus Subtilis Rskk243 Bifunctional Xylanase Gene In E. Coli And B. Subtilis And Enzyme Characterization, Bahri̇ Devri̇m Özcan, Numan Özcan
Cloning Of Bacillus Subtilis Rskk243 Bifunctional Xylanase Gene In E. Coli And B. Subtilis And Enzyme Characterization, Bahri̇ Devri̇m Özcan, Numan Özcan
Turkish Journal of Veterinary & Animal Sciences
A bifunctional gene xylanase of B. subtilis RSKK243 encoding detectable beta(1,4) glucanase (CMCase) activity in addition to a high level of main xylanase activity was cloned and expressed in Escherichia coli with pUC18 plasmid and in various Bacillus subtilis strains with pUB110 vector. For enzymatic analysis and plasmid isolation, the gene was also expressed in xylanase and beta glucanase negative B.subtilis YB886 strain with pUB110 vector. The pUB110 with bifunctional xylanase gene was transferred into B. subtilis RSKK243 for maximizing the enzyme yield, as well as into other RSKK strains to create novel strains with multiple enzyme capacities. Recombinant strains …