Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 1 of 1
Full-Text Articles in Life Sciences
Molecular Cloning And Analysis Of The Promoter For Rat Hepatic Neutral Cholesterol Ester Hydrolase, Ramesh Natarajan
Molecular Cloning And Analysis Of The Promoter For Rat Hepatic Neutral Cholesterol Ester Hydrolase, Ramesh Natarajan
Theses and Dissertations
Neutral cholesterol ester hydrolase (CEH) is a key enzyme in regulating hepatic free cholesterol. Using the CEH specific cDNA sequence in the 5'-untranslated region as a primer, 1.3 kb of sequence upstream of the ATG initiation codon was amplified and cloned. Primer extension analysis with total RNA from rat primary hepatocytes identified a transcription initiation site, 60 bases upstream from the initiation codon. No typical TATA-box sequences were found upstream from the transcription start site. However, a consensus GC-box, which can bind the positive transcription factor SP1, was found 35 bases upstream from the transcription start site. In addition the …