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Articles 1 - 4 of 4
Full-Text Articles in Life Sciences
Time-Resolved Cryofixation Methods For The Study Of Dynamic Cellular Events By Electron Microscopy: A Review, Keith P. Ryan, Gerd Knoll
Time-Resolved Cryofixation Methods For The Study Of Dynamic Cellular Events By Electron Microscopy: A Review, Keith P. Ryan, Gerd Knoll
Scanning Microscopy
The preservation of cells for electron microscopy by chemical fixation is a lengthy process, requiring up to 30 minutes for cytoplasmic stabilisation. This time lag enables many changes to occur in specimens so that they may not reflect their living state when they are observed in electron microscopes. Many artefacts can be avoided by using cryofixation, which freezes specimens over a period that is measured in milliseconds, so that specimens are preserved by cryoimmobilisation. This time resolution can be used to study rapid processes in biology and chemistry because, although electron microscopes cannot observe dynamic cellular events directly, processes can …
Ultrastructural Features Of Apoptosis, Elisabetta Falcieri, Pietro Gobbi, Loris Zamai, Marco Vitale
Ultrastructural Features Of Apoptosis, Elisabetta Falcieri, Pietro Gobbi, Loris Zamai, Marco Vitale
Scanning Microscopy
Apoptosis is a gene-directed physiological and programmed process of cell deletion aimed at the regulation of tissue and organ development. It affects different cell types and is triggered by a variety of stimuli all inducing closely comparable structural changes. Despite the deeply different morphology and metabolism of the cell models and the various inducers and their initial effects, a convergence seems to take place in a common metabolic pathway that, in most cases, involves the activation of a Ca2+ dependent endonuclease. A growing body of data is now available on the molecular events that lead to DNA damage. DNA …
Ultrastructural Observations Of The Argonaut Shell, P. R. Mitchell, P. P. Phakey, W. A. Rachinger
Ultrastructural Observations Of The Argonaut Shell, P. R. Mitchell, P. P. Phakey, W. A. Rachinger
Scanning Microscopy
An examination of the ultrastructure of the shell of the cephalopod Argonauta Nodosa was carried out using scanning electron microscopy, transmission electron microscopy and polarised light microscopy. The structure of the Argonaut shell was found to consist of an inner and outer prismatic layer separated by a thin central zone which was sparsely occupied by spherulitic crystals. Fluctuations in the width and porosity of the central zone resulted in changes in the shell's opacity and gave rise to the fibrous lines visible in the structure. The central zone was the region of initial growth and was the nucleating point for …
Binding Of Matrix Attachment Regions To Lamin Polymers Involves Single-Stranded Regions And The Minor Groove., M. E. Eva Ludérus, Jan L. Den Blaauwen, Oncko J. De Smit, Duane A. Compton, Roel Van Driel
Binding Of Matrix Attachment Regions To Lamin Polymers Involves Single-Stranded Regions And The Minor Groove., M. E. Eva Ludérus, Jan L. Den Blaauwen, Oncko J. De Smit, Duane A. Compton, Roel Van Driel
Dartmouth Scholarship
Chromatin in eukaryotic nuclei is thought to be partitioned into functional loop domains that are generated by the binding of defined DNA sequences, named MARs (matrix attachment regions), to the nuclear matrix. We have previously identified B-type lamins as MAR-binding matrix components (M. E. E. Ludérus, A. de Graaf, E. Mattia, J. L. den Blaauwen, M. A. Grande, L. de Jong, and R. van Driel, Cell 70:949-959, 1992). Here we show that A-type lamins and the structurally related proteins desmin and NuMA also specifically bind MARs in vitro. We studied the interaction between MARs and lamin polymers in molecular detail …