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Development And Standardization Of A Short-Term Assay For Evaluating Polluted Estuarine And Coastal Environments: The Medaka Embryo-Larval Assay, Michael Frederick Helmstetter
Development And Standardization Of A Short-Term Assay For Evaluating Polluted Estuarine And Coastal Environments: The Medaka Embryo-Larval Assay, Michael Frederick Helmstetter
OES Theses and Dissertations
The eggs of the Japanese medaka (Oryzias latipes) were employed in a number of studies to develop a routine, standardized assay which can assess the acute and sublethal impacts of individual toxicants and complex mixtures. The eggs of this Cyprinodont minnow were topically treated with each toxicant or mixture dissolved in membrane permeable dimethylsulfoxide (DMSO) solvent. Nine chemicals were initially evaluated with two, pentachlorophenol (PCP) and tributyltin chloride (TBTCl), subsequently selected for extensive evaluations of salinity tolerance and the accuracy of the topical exposure method for representing a typical immersion exposure. The amount of material actually penetrating the eggs was …