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Full-Text Articles in Life Sciences
Chromatin Structure And Gene Activity, Sarah C.R. Elgin
Chromatin Structure And Gene Activity, Sarah C.R. Elgin
Biology Faculty Publications & Presentations
No abstract provided.
Conservation Of Intron Position Indicates Separation Of Major And Variant H2as Is An Early Event In The Evolution Of Eukaryotes, A Van Daal, E White, Sarah C.R. Elgin, M Gorovsky
Conservation Of Intron Position Indicates Separation Of Major And Variant H2as Is An Early Event In The Evolution Of Eukaryotes, A Van Daal, E White, Sarah C.R. Elgin, M Gorovsky
Biology Faculty Publications & Presentations
Genomic clones of Drosophila and Tetrahymena histone H2A variants were isolated using the corresponding cDNA clones (van Daal et al. 1988; White et al. 1988). The site corresponding to the initiation of transcription was defined by primer extension for both Drosophila and Tetrahymena genomic sequences. The sequences of the genomic clones revealed the presence of introns in each of the genes. The Drosophila gene has three introns: one immediately following the initiation codon, one between amino acids 26 and 27 (gln and phe), and one between amino acids 64 and 65 (glu and val). The Tetrahymena gene has two introns, …
Optimal Heat-Induced Expression Of The Drosophila Hsp26 Gene Requires A Promoter Sequence Containing (Ct)N.(Ga)N Repeats, R Glaser, Graham H. Thomas, Ester Siegfried, Sarah C.R. Elgin, J Lis
Optimal Heat-Induced Expression Of The Drosophila Hsp26 Gene Requires A Promoter Sequence Containing (Ct)N.(Ga)N Repeats, R Glaser, Graham H. Thomas, Ester Siegfried, Sarah C.R. Elgin, J Lis
Biology Faculty Publications & Presentations
We report here the analysis of the sequence requirements for the heat-induced expression of the Drosophila melanogaster hsp26 gene using germline transformation. Heat-induced expression is augmented fivefold by a homopurine/homopyrimidine region from -85 to -134 that is devoid of heat-shock elements but contains numerous (dC-dT).(dG-dA) repeats. Sequences within this interval have been shown to assume a nuclease S1-hypersensitive structure in vitro. In this paper, we extend those in vitro observations, demonstrating that the S1-hypersensitive structure is triple-helical H-DNA formed by a symmetric (dC-dT).(dG-dA) sequence. Thus, the sequences that form H-DNA in vitro are also required in vivo for optimal hsp26 …