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Presence Of A Cs-Resistant Transient Outward Current Which Distorts Ica Measurements In Guinea-Pig Single Ventricular Cells, Georges Christé, Brigitte Delachapelle, Fouad Lemtiri-Chlieh, Carlos Ojeda
Presence Of A Cs-Resistant Transient Outward Current Which Distorts Ica Measurements In Guinea-Pig Single Ventricular Cells, Georges Christé, Brigitte Delachapelle, Fouad Lemtiri-Chlieh, Carlos Ojeda
fouad Lemtiri-Chlieh
To correctlyr analyse the physical properties of ion channels and the actions of drugs upon them, it is important that the membrane conducts only the ionic species believed permeant. In guinea-pig ventricular cells it has been assumed that only ICaL flows in the potential range -50 to +60 mV in the presence of intra- and extracellular Cs (> 20 mM). We report here that this is not the case due to the presence of a Cs-insensitive, transient outward current.
Measurements Of Cytosolic Free Ca In Cultured Rat Neonate And Adult Guinea-Pig Ventricular Cardiac Myocytes, Jean-Claude Bernengo, Fouad Lemtiri-Chlieh, Carlos Ojeda, Nadine Pltonoff
Measurements Of Cytosolic Free Ca In Cultured Rat Neonate And Adult Guinea-Pig Ventricular Cardiac Myocytes, Jean-Claude Bernengo, Fouad Lemtiri-Chlieh, Carlos Ojeda, Nadine Pltonoff
fouad Lemtiri-Chlieh
Cytosolic free Ca was measured with Indo-1 (Grynkiewicz et al. 1985) as free acid (in guinea-pig cells) or as the ester (in rat neonate cells). Using image analysis we obtained concentration maps at 40 ms intervals at two wavelengths (Cannell et al. 1986). In rat neonate cells, at rest and during contraction, calcium was apparently non-uniformly distributed, in contrast to guinea-pig adult cells. Using a dual PM spectroscopic system adapted to an inverted microscope we found: (1) the conversion of Indo-AM to Indo- 1 in the cytosol is not complete (< 40% contribution to the total light); (2) rat neonate cells buffer Ca (at rest 130-170 nM) only for [Ca]. between 1 and 3 mM, whereas in adult guinea-pig cells the range extended to 10 mM; (3) during contraction [Ca]i rose from 200 nm to no more than 1 microM. Digitoxin increases basal and peak [Ca]i; (4) during spontaneous contractions, in guinea-pig cells, [Ca]i rises from 70 to 200 nm when [Ca]o = 0, and from 170 nm to about 1 microM when [Ca]o = 2 mM; (5) in voltage-clamp conditions the rise in [Ca]i does not exceed 2 microM and oscillations occur in the presence of forskolin. When loading with Indo-1, cells frequently cease to contract (Powell et al. 1988) and the variations in [Ca]i are then very small (about 200 nm at peak ICa). In both cases these values are small compared to the total charge crossing the membrane.
Amino Acid Uptake And Metabolism By Larvae Of The Marine Worm Urechis Caupo (Echiura), A New Species In Axenic Culture, William Jaeckle, Donal T. Manahan
Amino Acid Uptake And Metabolism By Larvae Of The Marine Worm Urechis Caupo (Echiura), A New Species In Axenic Culture, William Jaeckle, Donal T. Manahan
William Jaeckle
Axenic (bacteria-free) larval cultures of the marine echiuran worm, Urechis caupo, were reliably obtained by aseptically removing gametes directly from the gamete storage organs. Trochophore larvae only removed neutral amino acids from seawater as measured by high-performance liquid chromatography (HPLC). There was no detectable uptake, as measured by HPLC, of acidic or basic amino acids. Kinetic analysis showed that the transport system for alanine in 4-day-old larvae had a Kt of 4-6 μM and a Jmax of 9-10 pmol larva-1 h-1. Following a 50-min exposure, the majority of the radio-activity (95%) from 14C-alanine was found in the trichlo-roacetic acid-soluble fraction. …
Ontogenic Changes In The Rates Of Amino Acid Transport From Seawater By Marine Invertebrate Larvae (Echinodermata, Echiura, Mollusca), Donal Manahan, William Jaeckle, Saeid Nourizadeh
Ontogenic Changes In The Rates Of Amino Acid Transport From Seawater By Marine Invertebrate Larvae (Echinodermata, Echiura, Mollusca), Donal Manahan, William Jaeckle, Saeid Nourizadeh
William Jaeckle
Transport rates of amino acids were determined for larvae of different ages of the echiuran worm Urechis caupo, the gastropod Haliotis rufescens, the bivalve Crassostrea gigas, and the sea urchin Strongylocentrotus purpuratus. All larval forms showed an increase in the transport rate of amino acids during development. Trochophores of U. caupo increased their rate of net flux for each of 5 amino acids (100 nM each) by a factor of 1.6 and 2.2 during 1-3 days and 4-8 days, respectively, for two independent cultures. In H. rufescens, the maximum transport capacity (Jmax) for alanine increased 3-fold during the 24 h …
Feeding By A “Nonfeeding” Larva: Uptake Of Dissolved Amino Acids From Seawater By Licithotrophic Larvae Of The Gastropod Haliotis Refescens, William Jaeckle
Feeding By A “Nonfeeding” Larva: Uptake Of Dissolved Amino Acids From Seawater By Licithotrophic Larvae Of The Gastropod Haliotis Refescens, William Jaeckle
William Jaeckle
No abstract provided.
Growth And Energy Imbalance During The Development Of A Lecithotrophic Molluscan Larva (Haliotis Rufescens), William Jaeckle, Donal T. Manahan
Growth And Energy Imbalance During The Development Of A Lecithotrophic Molluscan Larva (Haliotis Rufescens), William Jaeckle, Donal T. Manahan
William Jaeckle
Larvae of the gastropod Haliotis rufescens are classified as "nonfeeding" because they cannot capture particular foods. However, for only 1 out of 5 independent cultures was a net decrease observed in dry organic weight during the complete period of larval development (5 to 7 days). In fact, there were net increases in dry organic weight from the oocyte (day 0) to the newly formed veliger larvae (2-day-old). These weight increases during early development could be explained by increases in the amounts of specific biochemical components of the larvae, relative to oocytes. The metabolic rates of larvae were measured (oxygen consumption) …