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Full-Text Articles in Life Sciences
Studies Of Heart Development In Normal And Cardiac Lethal Mutant Axolotls: A Review, M. E. Fransen, L. F. Lemanski
Studies Of Heart Development In Normal And Cardiac Lethal Mutant Axolotls: A Review, M. E. Fransen, L. F. Lemanski
Scanning Microscopy
The morphology of developing hearts in axolotls, Ambystoma mexicanum, has been studied by scanning electron microscopy in order to provide a chronology of morphogenesis that can be correlated with ongoing biochemical and immunocytochemical studies. In addition to normal embryos we have studied homozygous recessive cardiac lethal mutant axolotls. The mutant myocardium undergoes aberrant sarcomere development and lacks a normal heartbeat. Morphogenesis of mutant hearts appears to be nearly normal with respect to myocardial cell shape changes, epicardial formation, and the distribution of extracellular matrix fibrils in the cardiac jelly. This suggests that the deficient arrangement of contractile proteins in …
Microprobe Analysis Of Element Distribution In Bovine Extracellular Matrices And Muscle, M. B. Engel, H. R. Catchpole
Microprobe Analysis Of Element Distribution In Bovine Extracellular Matrices And Muscle, M. B. Engel, H. R. Catchpole
Scanning Microscopy
The concentrations of some essential elements, Na, K, P, S and Cl were determined by microprobe analysis in bovine extracellular matrices of cartilage, tendon and elastic tissue (ligamentum nuchae) and in muscle cells. The values for the different tissues were compared and related to the blood electrolyte concentrations. Among the connective tissues the highest Na and lowest Cl values were found for cartilage which bears a high negative charge. The lowest concentrations of these elements occurred in elastic tissue which is relatively non-polar. In the three extracellular matrices sodium levels exceeded potassium. In myofibers potassium was the major cation at …
Effects Of Extracellular Matrix On Cytoskeletal And Myofibrillar Organization In Vitro, Lula L. Hilenski, Louis Terracio, Roger Sawyer, Thomas K. Borg
Effects Of Extracellular Matrix On Cytoskeletal And Myofibrillar Organization In Vitro, Lula L. Hilenski, Louis Terracio, Roger Sawyer, Thomas K. Borg
Scanning Microscopy
The distribution and three-dimensional relationship of myofibrillar and cytoskeletal components during myofibrillogenesis were examined in preparations of neonatal rat cardiac myocytes processed in parallel for scanning electron microscopy (SEM), intermediate voltage transmission electron microscopy (IVEM) and immunofluorescence (IF). Of the various methods used for processing, optimal results were achieved by pre-extraction with Triton X-100 in an actin-stabilizing buffer. This procedure effectively removed the surface membrane, as viewed by SEM images, while preserving myofibrillar and cytoskeletal structure, as evidenced by IF for actin, 𝝰-actinin and vinculin. Cytoskeletons in SEM images consisted of a cortex of anastomosing filaments through which ran parallel …