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1987

Cell Biology

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Mcnamara 1987 Uiuc Phd Dissertation - Morphogenetic Movements Contributing To Cell Aggregation In Dictyostelium Discoideum Wild-Type And Streamer F Mutants, George Mcnamara Apr 1987

Mcnamara 1987 Uiuc Phd Dissertation - Morphogenetic Movements Contributing To Cell Aggregation In Dictyostelium Discoideum Wild-Type And Streamer F Mutants, George Mcnamara

George McNamara

McNamara 1987 UIUC PhD Dissertation - Morphogenetic movements contributing to cell aggregation in Dictyostelium discoideum wild-type and streamer F mutants.
PhD Supervisor: Robert P. Futrelle.
We investigated some cell processes which may contribute to cell aggregation patterns in the social ameba Dictyostelium discoideum. Aggregation is known to be mediated by chemotaxis up periodic extracellular cAMP gradients; however, the details of cell movement during this stage of development have not been reported. We recorded cell behavior by time-lapse cinemicroscopy and collected cell motion and cell shape data by computer-aided planimetry.
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Bottom line is that I discovered that the reason streamerF …

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Fine Structure Of The Substantia Nigra Pars Lateralis In The Opossum, Terrence Paul Ma Jan 1987

Fine Structure Of The Substantia Nigra Pars Lateralis In The Opossum, Terrence Paul Ma

Wayne State University Dissertations

The cytoarchitecture of the substantia nigra pars lateralis (SNl) of the opossum was examined by light and electron microscopic procedures. Nissl-stained, Golgi-impregnated, HRP-reacted and electron microscopic preparations were utilized in this study. SNl neurons were divided into three groups on the basis of cell size; small, medium-sized, and large neurons. Small cells had a nucleus with some clumped heterochromatin, a relatively organelle-free cytoplasm, and long, thin, sparcely branching dendrites. Electron microscopic examination of small cells confirmed the light microscopic observations. Medium-sized neurons possessed a relatively clear nucleus, clumps of Nissl substance, especially at a juxtanuclear position near the emergence of …

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Short-Term Feedback Regulation Of Camp By Accelerated Degradation In Rat Tissues, Tom W. Gettys, Peter F. Blackmore, J. Bruce Redmon, Stephen J. Beebe, Jackie D. Corbin Jan 1987

Short-Term Feedback Regulation Of Camp By Accelerated Degradation In Rat Tissues, Tom W. Gettys, Peter F. Blackmore, J. Bruce Redmon, Stephen J. Beebe, Jackie D. Corbin

Bioelectrics Publications

A recent study showed that cAMP analogs lowered cAMP levels in rat hepatocytes. The present work demonstrates that cAMP analogs also lowered cAMP in a rapid, concentration-dependent manner in heart and fat cells. In order to determine if the cAMP-dependent protein kinase mediated this effect, techniques were developed to assay the protein kinase activity ratio in hepatocytes treated with cAMP analogs. The activation of protein kinase and phosphorylase in hepatocytes by 8-pClΦS-cAMP (where 8-pClΦS- indicates 8-parachlorothiophenyl-) was concentration-dependent and occurred in parallel to proportionate decreases in cAMP. More than 20% of the cAMP binding sites on the protein kinase were …

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