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Full-Text Articles in Life Sciences
Selenium-Induced Enhancement Of Hematoporphyrin Derivative Phototoxicity In Murine Bladder Tumor Cells, Jeffrey M. Tosk
Selenium-Induced Enhancement Of Hematoporphyrin Derivative Phototoxicity In Murine Bladder Tumor Cells, Jeffrey M. Tosk
Loma Linda University Electronic Theses, Dissertations & Projects
The phototoxicity of hematoporphyrin derivative (Hpd) to murine bladder tumor (MBT-2) cells was studied in vitro. It was observed that selenium in the form of sodium selenite enhanced Hpd-sensitized photodamage in MBT-2 cells under conditions where the selenite alone was non-toxic. Sodium selenite was found to enhance the fluorescence emission of Hpd and augment the Hpd-sensitized photooxidation of tryptophan. The data indicate that sodium selenite is able to disaggregate Hpd, thereby enhancing Hpd-sensitized phototoxicity.
The Effect Of Dietary Fat Level On Tumor Development And Immunity In Balb/C Mice Fed Casein Of Wheat Gluten As Protein And Challenged With Herpes Type 2-Transformed Cells, Helen Drakou
Loma Linda University Electronic Theses, Dissertations & Projects
A high percentage of fat in the diet has been implicated through epidemiological studies in several human cancers. Studies in experimental animals have produced evidence that increasing the fat in the diet decreases the animal’s ability to restrict tumor development and growth. In previous work done in this laboratory with BALB/c mice injected with herpes simplex Type 2-transformed mouse cells (H238), the effects of a diet which greatly enhanced tumor growth was compared with one which restricted tumor growth. The latter, a low (5%) fat, low protein diet, in which the protein was casein, was also found to produce a …
The Expression Of Dna Restriction-Modification Genes Of Escherichia Coli K12, Ainu Prakash
The Expression Of Dna Restriction-Modification Genes Of Escherichia Coli K12, Ainu Prakash
Loma Linda University Electronic Theses, Dissertations & Projects
To facilitate the study of the expression of hsd genes in Escherichia coli K, hsdK::MudX (amp crop lac) operon fusion mutants have successfully been constructed and characterized for the first time. The results indicate that Promoters 1 and 2 for hsdK genes appear to be relatively weak, as reflected by about 50 units of beta-gal activity. Both promoters were active in all the conditions tested except during Beta-gal activity was higher in aerobic than in anaerobic growth; at 30°C and 37°C than at 42°C; and in L-broth early log phase of the culture. than in minimal media. Potential future uses …
Sprague-Dawley Rats Fed Milk Or Beef Protein : Differences In Response To 1,2-Dimethylhydrazine Carcinogenesis, Stanley W. L. Ng
Sprague-Dawley Rats Fed Milk Or Beef Protein : Differences In Response To 1,2-Dimethylhydrazine Carcinogenesis, Stanley W. L. Ng
Loma Linda University Electronic Theses, Dissertations & Projects
Colon cancer is responsible for a high percentage of cancer deaths in developing countries, and there is convincing epidemiological evidence that meat protein and fat in the diet increase the incidence of this form of cancer. An animal tumor model which has been used by many investigators interested in colon cancer is the 1,2-dimethylhydrazine (DMH)-rat or DMH-mouse tumor model. In previous work done in this laboratory the DMH-BALB/c mouse tumor model was used to compare the production of colon tumors in mice fed a diet using non-fat powdered milk as a source of protein with those in mice fed beef …
The Effects Of Certain Oral Bacteria On The In Vitro Growth Of Bacteroides Gingivalis, Steven G. Morrow
The Effects Of Certain Oral Bacteria On The In Vitro Growth Of Bacteroides Gingivalis, Steven G. Morrow
Loma Linda University Electronic Theses, Dissertations & Projects
The effect of nine gram positive and grain negative cocci and bacilli, which are indigenous to the oral cavity, on the in vitro growth of Bacteroides gingivalis was examined in this study. A pure culture of B. gingivalis is was obtained from the American Type Culture Collection. The nine oral test organisms were Streptococcus mitis, Streptococcus mutans, Streptococcus faecalis, Staphylococcus epidermidis, Staphylococcus aureus, Corynebacteriurn hofmanii, Lactobacillus acidophilus, Branhamella catarrhalis, and Escherichia coli. The effect on the growth of B. gingivalis of the nine test organisms was evaluated on a solid medium by use of …
Augmentation Of Natural Killer Cell Activity By Corynebacterium Parvum: Involvement Of Lipoxygenase Pathway, Gregory D. Kuo
Augmentation Of Natural Killer Cell Activity By Corynebacterium Parvum: Involvement Of Lipoxygenase Pathway, Gregory D. Kuo
Loma Linda University Electronic Theses, Dissertations & Projects
The 51Chromium release assay was used to measure natural killer (NK) cell activity associated with Corynebacterium parvum (CP) injection against YAC-1 and MBT-2 targets. The peritoneal exudate cells from intraperitoneal treatment of CP at different dosages consistently gave higher cytotoxicity than their respective spleen cell fractions. In contrast, CP given intravenously showed the reversed effect. Our results demonstrate that the route of administration and dosage of CP are two crucial variables for determining the effectiveness of this immunostimulant. In vitro manipulations of nordihydroguaiaretic acid, indomethacin, and prostaglandin E2 on CP-stimulated NK cells suggest that the lipoxygenase pathway is involved …
Restriction Analysis Of Bacteriophage, Ali Reza Mostaghimi
Restriction Analysis Of Bacteriophage, Ali Reza Mostaghimi
Loma Linda University Electronic Theses, Dissertations & Projects
A restriction map of DNA from bacteriophage P3-C1 for the endonucleases BamH I, Bgl II, EcoR I, Hind III, Sac I and Sca I is described in this study. The phage DNA was determined to be a linear double stranded molecule with a unit genomic length of 31 kb. After electrophoresis in 0.5% agarose gels, the undigested DNA appeared as three molecules of 31, 62 and 93 kb in length. The Hind III digestion pattern of the individual 31 kb molecule was identical to the 62 kb and 93 kb Hind III pattern. With all enzymes except Hind III, …