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Full-Text Articles in Life Sciences
The Isolation Of Ecdysterone Inducible Genes By Hybridization Subtraction Chromatography, Michael P. Vitek, Susan G. Kreissman, Robert H. Gross
The Isolation Of Ecdysterone Inducible Genes By Hybridization Subtraction Chromatography, Michael P. Vitek, Susan G. Kreissman, Robert H. Gross
Dartmouth Scholarship
We have developed a procedure for selectively enriching a mRNA population for inducible sequences. Other than the induced mRNA species, the population of mRNA in control cells is approximately the same as the mRNA population in induced cells. Cytoplasmic mRNA from control cells is bound to oligo (dT)-cellulose and used as a template for reverse transcriptase, the oligo (dT) serving as a primer. After removing the template mRNAs, the cDNA-cellulose column is used to hybridize a population of mRNAs from induced cells. The non-hybridized poly A + RNAs are greatly enriched in the inducible sequences. We have used this technique …
The Effect Of Temperature On The Life Cycle Of Drosophila Acutilabella, Donald A. Miles
The Effect Of Temperature On The Life Cycle Of Drosophila Acutilabella, Donald A. Miles
University of the Pacific Theses and Dissertations
This is a study of the rate of development of the fly, Drosophila acutilabelle, Stalker (1953), at three temperatures, 22°, 25°, and 28°C. It is part of a larger project testing the hypothesis that stenothermal species (those restricted to a narrow temperature range) are more sensitive than eurythermal species, such a the cosmopolitan D. melanogaster and D. hydei (those able to live over a broad temperature range) to the effects of temperature during development. It has been suggested that eurythermal species have a lower Q10 of development. The species used in this study is considered to be stenothermal because …