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Microarray Analysis Of Arabidopsis Wrky33 Mutants In Response To The Necrotrophic Fungus Botrytis Cinerea, Shamma Saeed Salem Al Shamsi
Microarray Analysis Of Arabidopsis Wrky33 Mutants In Response To The Necrotrophic Fungus Botrytis Cinerea, Shamma Saeed Salem Al Shamsi
Theses
Plants are exposed to many environmental stresses that affect their growth and development. These stresses include biotic stresses (via organisms) and abiotic stresses (via environment). Plants respond to these stresses by transcriptional reprogramming and different signaling pathways. Arabidopsis thaliana has shown great sensitivity to the biotic stress Botrytis cinerea. The WRKY33 gene plays an important role in plant defense mechanism against this pathogen. The overall goal is to identify common regulated genes of wrky33 mutant and 35S:WRKY33 over expressing transgenic lines in response to B. cinerea; ultimately to improve plant stress tolerance in Arabidopsis. The specific aims are to: (1) …
Identification Of Arabidopsis Candidate Genes In Response To Biotic And Abiotic Stresses Using Comparative Microarrays, Salma Omar Al Ameri
Identification Of Arabidopsis Candidate Genes In Response To Biotic And Abiotic Stresses Using Comparative Microarrays, Salma Omar Al Ameri
Theses
Plants have evolved with intricate mechanisms to cope with multiple environmental stresses. To adapt with biotic and abiotic stresses, plant responses involve changes at the cellular and molecular levels. The current study was designed to investigate the effects of combinations of different environmental stresses on the transcriptome level of Arabidopsis thaliana genome using public microarray databases. We investigated the role of cyclopentenones in mediating plant responses to environmental stress through TGA (TGACG motif-binding factor) transcription factor, independently from jasmonic acid. Candidate genes were identified by comparing plants inoculated with Botrytis cinerea or treated with heat, salt or osmotic stress with …
Transcriptomes Analysis Reveals Genes Commonly Induced By Botrytis Cinerea Infection, Cold, Drought And Oxidative Stresses In Arabidopsis Thaliana, Ahmed Dawood Al-Azzawi
Transcriptomes Analysis Reveals Genes Commonly Induced By Botrytis Cinerea Infection, Cold, Drought And Oxidative Stresses In Arabidopsis Thaliana, Ahmed Dawood Al-Azzawi
Theses
Signaling pathways controlling biotic and abiotic stress responses may interact synergistically or antagonistically. To identify the similarities and differences among responses to diverse stresses, we analyzed previously published microarray data on the transcriptomic responses of Arabidopsis thaliana to infection with Botrytis cinerea (a biotic stresses). Our analyses showed that at least at early stages B. cinerea inoculation, 1498 genes were up-regulated (B. cinerea up-regulated genes; BUGS) and 1138 genes were down-regulated (B. cinerea down-regulated genes; BUGS). We showed a unique program of gene expression was activated in response each biotic and abiotic stress, but that …
Trascriptome Analysis Reveals Genes Commonly Induced By Botrytis Cinerea Infection, Cold, Drought And Oxidative Stresses In Arabidopsis Thaliana, Ahmed Dawood Al-Azzawi Al-Azzawi
Trascriptome Analysis Reveals Genes Commonly Induced By Botrytis Cinerea Infection, Cold, Drought And Oxidative Stresses In Arabidopsis Thaliana, Ahmed Dawood Al-Azzawi Al-Azzawi
Theses
Signaling pathways controlling biotic and abiotic stress responses may interact synergistically or antagonistically. To identify the similarities and differences among responses to diverse stresses, we analyzed previously published microarray data on the transcriptomic responses of Arabidopsis thaliana to infection with Botrytis cinerea (a biotic stress), and to cold, drought, and oxidative stresses (abiotic stresses). Our analyses showed that at early stages after B. cinerea inoculation, 1498 genes were up-regulated (B. cinerea up-regulated genes; BUGs) and 1138 genes were down-regulated (B. cinerea down-regulated genes; BDGs). We showed a unique program of gene expression was activated in response each biotic and abiotic …