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2006 Nebraska Swine Report, Duane E. Reese
2006 Nebraska Swine Report, Duane E. Reese
University of Nebraska-Lincoln Extension: Historical Materials
This publication was prepared by the staff in Animal Science and cooperating Departments for use in Extension, Teaching and Research programs. It deals with the results that were done in reproduction, breeding, health, nutrition, genetics, economics and housing of swine.
Comparison Of Virulence Gene Profiles Between Escherichia Coli Strains Isolated From Healthy And Diarrheic Swine, Mark R. Wilson, Xi Yang Wu, Idris Barchia, Karl A Bettelheim, Steven Driesen, Darren J. Trott, Toni A. Chapman, James J C Chin
Comparison Of Virulence Gene Profiles Between Escherichia Coli Strains Isolated From Healthy And Diarrheic Swine, Mark R. Wilson, Xi Yang Wu, Idris Barchia, Karl A Bettelheim, Steven Driesen, Darren J. Trott, Toni A. Chapman, James J C Chin
Faculty of Science - Papers (Archive)
A combination of uni- and multiplex PCR assays targeting 58 virulence genes (VGs) associated with Escherichia coli strains causing intestinal and extraintestinal disease in humans and other mammals was used to analyze the VG repertoire of 23 commensal E. coli isolates from healthy pigs and 52 clinical isolates associated with porcine neonatal diarrhea (ND) and postweaning diarrhea (PWD). The relationship between the presence and absence of VGs was interrogated using three statistical methods. According to the generalized linear model, 17 of 58 VGs were found to be significant (P < 0.05) in distinguishing between commensal and clinical isolates. Nine of the 17 genes represented by iha, hlyA, aidA, east1, aah, fimH, iroN(E. coli), traT, and saa have not been previously identified as important VGs in clinical porcine isolates in Australia. The remaining eight VGs code for fimbriae (F4, F5, F18, and F41) and toxins (STa, STb, LT, and Stx2), normally associated with porcine enterotoxigenic E. coli. Agglomerative hierarchical algorithm analysis grouped E. coli strains into subclusters based primarily on their serogroup. Multivariate analyses of clonal relationships based on the 17 VGs were collapsed into two-dimensional space by principal coordinate analysis. PWD clones were distributed in two quadrants, separated from ND and commensal clones, which tended to cluster within one quadrant. Clonal subclusters within quadrants were highly correlated with serogroups. These methods of analysis provide different perspectives in our attempts to understand how commensal and clinical porcine enterotoxigenic E. coli strains have evolved and are engaged in the dynamic process of losing or acquiring VGs within the pig population.
Ec06-219 2006 Nebraska Swine Report, Duane Reese
Ec06-219 2006 Nebraska Swine Report, Duane Reese
University of Nebraska-Lincoln Extension: Historical Materials
The 2006 Nebraska Swine Report was prepared by the staff in Animal Science and cooperating Departments for use in Extension, Teaching and Research programs. This publications deals with research on swine reproduction, breeding, health, nutrition, economics, and housing.