Life Sciences Commons^™

Daughter Cells Of Saccharomyces Cerevisiae From Old Mothers Display A Reduced Life Span, Nicanor Austriaco, Brian K. Kennedy, Leonard Guarente

Biology Faculty Publications

The yeast Saccharomyces cerevisiae typically divides asymmetrically to give a large mother cell and a smaller daughter cell. As mother cells become old, they enlarge and produce daughter cells that are larger than daughters derived from young mother cells. We found that occasional daughter cells were indistinguishable in size from their mothers, giving rise to a symmetric division. The frequency of symmetric divisions became greater as mother cells aged and reached a maximum occurrence of 30% in mothers undergoing their last cell division. Symmetric divisions occurred similarly in rad9 and ste12 mutants. Strikingly, daughters from old mothers, whether they arose …

Ua66/14/4 Kentucky Partnership Newsletter, Vol. Ii, No. 6, Kentucky Partnership For Farm Family Health & Safety

University Organizations

Newsletter created by and about the Kentucky Partnership for Farm Family Health & Safety.

1994 Scholars And Artists Bibliography, Michael Schwartz Library, Cleveland State University, Friends Of The Michael Schwartz Library

Scholars and Artists Bibliographies

This bibliography was created for the annual Friends of the Michael Schwartz Library Scholars and Artists Reception, recognizing scholarly and creative achievements of Cleveland State University faculty, staff and emeriti

Ua66/14/4 Kentucky Partnership Newsletter, Vol. Ii, No. 5, Kentucky Partnership For Farm Family Health & Safety

University Organizations

Newsletter created by and about the Kentucky Partnership for Farm Family Health & Safety.

Effects Of Natural Sequence Variation On Recognition By Monoclonal Antibodies Neutralize Simian Immunodeficiency Virus Infectivity, Weon Sang Choi, Catherine Collignon, Clotilde Thiriart, Dawn P. Wooley, E. J. Scott, Karen A. Kent, Ronald C. Desrosiers

Neuroscience, Cell Biology & Physiology Faculty Publications

The determinants of immune recognition by five monoclonal antibodies (KK5, KK9, KK17, Senv7.1, and Senv101.1) that neutralize simian immunodeficiency virus infectivity were analyzed. These five neutralizing monoclonal antibodies were generated to native SIVmac251 envelope glycoprotein expressed by a vaccinia virus recombinant vector. All five recognize conformational or discontinuous epitopes and require native antigen for optimal recognition. These monoclonal antibodies also recognize SIVmac239 gp120, but they do not recognize gp120 of two natural variants of SIVmac239, 1-12 and 8-22, which evolved during the course of persistent infection in vivo (D.P.W. Burns and R.C. Desrosiers, J. Virol. 65:1843-1854, 1991). Recombinant viruses which …

Peculiar Histopathological Features Of Giardiasis In Distal Duodenal Biopsies, Z Abbas, A A. Qureshi, H Sheikh, S M. Jafri, A H. Khan

Department of Pathology and Laboratory Medicine

Histological changes in 20 Giardia positive duodenal biopsies (Group A) were compared with 50, Giardia negative duodenal biopsies (Group B), taken during the same period. Stool examinations in Group B were negative for Giardia. Surface epithelium, villous and crypt architecture and cellular infiltrates were examined and compared between the groups. Atrophic changes in the villi were more common in Group A as compared to B(P < 0.0001). Intraepithelial neutrophil infiltration (P < 0.001), infiltration of the lamina propria with plasma cells (P < 0.5), and presence of eosinophils in the lamina propria (P < 0.001) were significant findings in group A. Some of the changes were related to the density of Giardia colonization e.g., the goblet cell depletion (P < 0.05) and the density of plasma cell infiltration in lamina propria (P < 0.01). Erosions and ulcerations were less commonly seen in group A. Thus we conclude that giardiasis manifests its peculiar features in the distal duodenal mucosa and a biopsy of this region is an important diagnostic tool for detection of this disease.

Ua66/14/4 Kentucky Partnership Newsletter, Vol. Ii, No. 4, Kentucky Partnership For Farm Family Health & Safety

University Organizations

Newsletter created by and about the Kentucky Partnership for Farm Family Health & Safety.

A Thyroid Hormone-Regulated Gene In Xenopus Laevis Encodes A Type Iii Iodothyronine 5-Deiodinase., Donald L. St Germain, Robert Schwartzman, Walburga Croteau, Akira Kanamori, Zhou Wang, Donald D. Brown, Valerie Galton

Dartmouth Scholarship

The type III iodothyronine 5-deiodinase metabolizes thyroxine and 3,5,3'-triiodothyronine to inactive metabolites by catalyzing the removal of iodine from the inner ring. The enzyme is expressed in a tissue-specific pattern during particular stages of development in amphibia, birds, and mammals. Recently, a PCR-based subtractive hybridization technique has been used to isolate cDNAs prepared from Xenopus laevis tadpole tail mRNA that represent genes upregulated by thyroid hormone during metamorphosis. Sequence analysis of one of these cDNAs (XL-15) revealed regions of homology to the mRNA encoding the rat type I (outer ring) 5'-deiodinase, including a conserved UGA codon that encodes selenocysteine in …

Immunohistochemistry In Tumour Diagnosis--Who Actually Needs It?, S Pervez

Department of Pathology and Laboratory Medicine

No abstract provided.

Adoptive Transfer Of Polyclonal And Cloned Cytolytic T Lymphocytes (Ctl) Specific For Mouse Aids-Associated Tumors Is Effective In Preserving Ctl Responses: A Measure Of Protection Against Lp-Bm5 Retrovirus-Induced Immunodeficiency., William R. Green, Kathy A. Green, Karen M. Crassi

Dartmouth Scholarship

Cytolytic T lymphocytes (CTL) can be raised against C57BL/6 B-cell lymphomas from mice with LP-BM5 murine leukemia virus-induced AIDS (MAIDS). Adoptive transfer of polyclonal anti-MAIDS tumor CTL or two CTL clones specific for the B6-1710 MAIDS lymphoma caused preservation of major histocompatibility complex-restricted and allogeneic CTL responses, which may be interpreted as indices of protection from LP-BM5 murine leukemia virus-induced immunodeficiency.

High Rates Of Frameshift Mutations Within Homo-Oligomeric Runs During A Single Cycle Of Retroviral Replication, Dawn P. Wooley, H. M. Temin

Neuroscience, Cell Biology & Physiology Faculty Publications

Homo-oligomeric runs were inserted into a spleen necrosis virus-based retrovirus vector to determine the nature and rate of mutations within runs of 10 to 12 identical nucleotides during a single replication cycle. Clones of helper cells containing integrated copies of retroviral vectors were used to produce virus for infection of target (nonhelper) cells. Proviral sequences from target cell clones were compared with proviral sequences from helper cell clones to study mutations that occurred during a single cycle of replication. In addition to the internal region spanning the homo-oligomeric inserts, a naturally occurring run of 10 T's in the long terminal …

Ua66/14/4 Kentucky Partnership Newsletter, Vol. Ii, No. 3, Kentucky Partnership For Farm Family Health & Safety

University Organizations

Newsletter created by and about the Kentucky Partnership for Farm Family Health & Safety.

Antibacterial Effects Of A Silver Electrode Carrying Microamperage Direct Current In Vitro, Poh Chye Young, Luther C. Kloth, Linda Jean Laatsch-Lybeck

Physical Therapy Faculty Research and Publications

Currently, electrical stimulation is an accepted method used clinically to promote chronic wound healing. A literature review revealed that similar therapeutic current has been shown to suppress growth of common wound pathogens in vitro and in vivo. To date, little has been reported on the factors contributing to the antibacterial effects of microamperage direct current (μADC) stimulation. The purpose of this project was to investigate the role of electric field strength, current density, pH, and type of electrode used in vitro, to gain a better understanding of how these factors contribute to inhibiting growth of select wound pathogens. …

Phylogenetic Associations Of Human And Simian T-Cell Leukemia/Lymphotropic Virus Type I Strains: Evidence For Interspecies Transmission, Igor J. Koralnik, Enzo Boeri, W. Carl Saxinger, Anita Lo Monico, Jake Fullen, Antoine Gessain, Hong-Guang Guo, Robert C. Gallo, Phillip Markham, Vaniambadi Kalyanaraman, Vanessa Hirsch, Jonathan Allan, Krishna Murthy, Patricia Alford, Jill Pecon-Slattery, Stephen J. O'Brien, Genoveffa Ranchini

Biology Faculty Articles

Homologous env sequences from 17 human T-leukemia/lymphotropic virus type I (HTLV-I) strains from throughout the world and from 25 simian T-leukemia/lymphotropic virus type I (STLV-I) strains from 12 simian species in Asia and Africa were analyzed in a phylogenetic context as an approach to resolving the natural history of these related retroviruses. STLV-I exhibited greater overall sequence variation between strains (1 to 18% compared with 0 to 9% for HTLV-I), supporting the simian origin of the modern viruses in all species. Three HTLV-I phylogenetic clusters or clades (cosmopolitan, Zaire, and Melanesia) were resolved with phenetic, parsimony, and likelihood analytical procedures. …

Ua66/14/4 Kentucky Partnership Newsletter, Vol. Ii, No. 2, Kentucky Partnership For Farm Family Health & Safety

University Organizations

Newsletter created by and about the Kentucky Partnership for Farm Family Health & Safety.

Amoebic Appendicitis--A Rare Entity, Raheem Ahmed, Hizbullah Shaikh, Muhammad Siddiqui, M Ahmed

Department of Pathology and Laboratory Medicine

Appendectomy is the most common surgical procedure performed in routine general surgical practice. However, not all the appendices removed, are submitted for histopathological examination in this part of the world. We reviewed 1400 appendices received by our department from within our hospital and from outside the hospital. Of these 13 cases were reported as amoebic appendicitis. These patients did not have any different clinical presentation from the patients who were reported as acute appendicitis without amoebae. Microscopically these appendices had minimal neutrophil polymorph infiltration accompanied by tissue necrosis and amoebic trophozoites within the appendiceal wall. After histological diagnosis, different tests …

Wild Medicine: Review Of Medicinal Wild Plants Of The Prairie By Kelly Kindscher, Kathleen H. Keeler

School of Biological Sciences: Faculty Publications

I am highly enthusiastic about this book as a reference work. It summarizes the literature on the medicinal uses for more than 103 prairie plants. For 43 species, Kindscher gives common, Indian, and scientific names; description; habitat; parts used; Indian uses; medical history; scientific research and cultivation; and information on about 100 of their relatives. Another 60 entries are condensed to 24 paragraphs. Full-page line drawings by William S. Whitney of the major species are generally excellent, and all 103 entries have distribution maps. The introduction discusses the region covered, with helpful maps. There's an index, a glossary and a …

The Amino-Terminal Functions Of The Simian Virus 40 Large T Antigen Are Required To Overcome Wild-Type P53-Mediated Growth Arrest Of Cells., Robin S. Quartin, Charles N. Cole, James M. Pipas, Arnold J. Levine

Dartmouth Scholarship

High levels of the p53 tumor suppressor protein can block progression through the cell cycle. A model system for the study of the mechanism of action of wild-type p53 is a cell line (T64-7B) derived from rat embryo fibroblasts transformed by activated ras and a temperature-sensitive murine p53 gene. At 37 to 39 degrees C, the murine p53 protein is in a mutant conformation and the cells actively divide, whereas at 32 degrees C, the protein has a wild-type conformation and the cells arrest in the G1 phase of the cell cycle. Wild-type simian virus 40 large T antigen and …

Serum Alpha 1 Antitrypsin In Liver Diseases, Anjum Shahid, Anwar Ali Siddiqui, Sarwar J. Zuberi, M A. Waqar

Department of Biological & Biomedical Sciences

No abstract provided.

Human Performance Lab Newsletter, February 1994, St. Cloud State University

Human Performance Lab Newsletter

Contents of this issue include:

• Kelly's Corner by Jack Kelly
• The Fit Philosopher by Sonya Hansen (about Prof. Myron Anderson)
• Heart at Work by Mary Kazemba
• HPL Research

Ua66/14/4 Kentucky Partnership Newsletter, Vol. Ii, No. 1, Kentucky Partnership For Farm Family Health & Safety

University Organizations

Newsletter created by and about the Kentucky Partnership for Farm Family Health & Safety.

Bcl-2 Protein Expression Is Widespread In The Developing Nervous-System And Retained In The Adult Pns, Diane E. Merry, Deborah J. Veis, William F. Hickey, Stanley J. Korsmeyer

Dartmouth Scholarship

Cell death is a common feature of neural development in all vertebrates. The bcl-2 proto-oncogene has been shown to protect a variety of cell types from programmed cell death. We have examined the distribution of bcl-2 protein in the developing and adult nervous systems. bcl-2 protein is widespread during embryonic development. Proliferating neuroepithelial cells of ventricular zones as well as the postmitotic cells of the cortical plate, cerebellum, hippocampus and spinal cord express bcl-2. Postnatally, bcl-2 is principally retained in the granule cells of the cerebellum and dentate gyrus of the hippocampus. bcl-2 expression in the CNS declines with aging. …

Separate Metabolic Pathways Leading To Dna Fragmentation And Apoptotic Nuclear Chromatin Condensation, D. Sun, Shibo Jiang, Li-Mou Zheng, David M. Ojcius, John Ding-E. Young

All Dugoni School of Dentistry Faculty Articles

Apoptosis is the predominant form of cell death observed in a variety of physiological and pathological conditions such as cancer involution, insect metamorphosis, the development of the immune and nervous systems, and embryogenesis. The typical nuclear changes taking place in apoptotic cells include extensive condensation of chromatin and internucleosomal DNA fragmentation into units of 200 base pairs. However, the mechanisms responsible for both chromatin condensation and DNA fragmentation have yet to be elucidated. In this study, micrococcal nuclease and the divalent cations, Ca2+ and Mg2+, were applied to isolated nuclei in an attempt to reconstitute in vitro the digestion of …

Morphometric Analysis Of Enteric Lesions In C3h/Hen Mice Inoculated With Serpulina Hyodysenteriae Serotypes 2 And 4 With Or Without Oral Streptomycin Pretreatment, Jagannatha V. Mysore, Gerald Duhamel

School of Veterinary and Biomedical Sciences: Faculty Publications

The segmental distribution and sequential progression and the role of the indigenous bacterial flora in the development of enteric lesions associated with Serpulina hyodysenteriae infection in laboratory mice have not been defined. We examined the distribution and sequential morphometric changes in the large intestine of mice orally inoculated with S. hyodysenteriae serotypes 2 and 4. To determine the role of colonization resistance conferred by the indigenous bacterial flora, 40 female C3H/HeN mice were administered water alone or water containing 5 mg/mL streptomycin sulfate ad libitum for seven days prior to orogastric inoculation either with S. hyodysenteriae or sterile trypticase soy …

Concepts Of Animal Well-Being And Predicting The Impact Of Procedures On Experimental Animals, D. J. Mellor, C. S. W. Reid

Experimental Research and Animal Welfare Collection

1. We argue that:

• in their application to non-human animals, 'welfare' and 'well-being' are interchangeable words; and that

• good welfare/well-being is the state of being manifest in an animal when its nutritional, environmental, health, behavioural and mental needs are met.

2. These latter are essentially the 'five freedoms' formulated by the Farm Animal Welfare Council of the United Kingdom.

3. Using the five freedoms as a basis, we have developed a system for assessing the impact of a proposed animal experiment or usage. The freedoms are now transformed into 'domains of potential compromise' and are redefined better to …

Ua64/10/5 Scrapbook 1989-1994, Wku Recreation Majors' Club

Student Organizations

Scrapbook created by members of the WKU Recreation Majors' Club between 1989 and 1994. Book mostly consists of photographs. There are a few event programs.

The Next Decade: A Shifting Focus, Henry Spira

Commentaries and Editorials

No abstract provided.

1994 Beef Cattle Report, Darrell W. Nelson

Nebraska Beef Cattle Reports

Two grazing trials were conducted to determine i/protein or energy was first limiting in the spring-and fall-born nursing calf grazing native sandhills range. Spring-born calves supplemented with escape protein gained more rapidly than calves supplemented with energy or nonsupplemented controls. Fall-born calves supplemented with escape protein gained more rapidly than those supplemented with rumen degradable protein or energy and nonsupplemented controls early in the trial, but gained at a similar rate to the energy controls by the end of the trial. Milk intake was similar across treatments for both trials. Escape protein is more limiting in the young nursing calf …

Sanitation And Parasitism At Harpers Ferry, West Virginia, Karl J. Reinhard

Karl Reinhard Publications

One focus of historical archaeology at Harpers Ferry National Historical Park, West Virginia, is tracing the development of sanitation at the town through the 1800s and 1900s. Historical documentation indicates that there was a degree of resistance to the modernization of village sanitation. This study attempts to verify this resistance through examination of privy soils for parasites indicative of fecalborne disease, specifically the helminth species Ascaris lumbricoides and Trichuris trichiura. The presence of these parasites in the early 1900s would indicate that fecal-borne disease due to poor sanitation continued to be an aspect of town life. The analysis revealed the …

Otitis Media, Mastoiditis, And Infracranial Lesions In Two Plains Indian Children, Robert W. Mann, Douglas W. Owsley, Karl J. Reinhard

Karl Reinhard Publications

One disease condition that is common to nearly all children is otitis media, also known as middle ear disease. Although most people speak of it as a middle ear "infection," other causes such as neoplasm (tumor) can result in manifestations and symptoms mimicking an infectious process. This paper presents findings related to osseous lesions in two Indian children, approximately three to five years of age, who died with otitis media and infracranial lesions. Through macroscopic and radiographic analysis, it is possible to show that one child probably suffered from tuberculosis and the other from histiocytosis X. From an epidemiological and …