Life Sciences Commons^™

Simian Virus 40 Host Range/Helper Function Mutations Cause Multiple Defects In Viral Late Gene Expression., Terryl Stacy, Michele Chamberlain, Charles N. Cole

Dartmouth Scholarship

Simian virus 40 (SV40) deletion mutants dlA2459 and dlA2475 express T antigens that lack the normal carboxy terminus. These mutants are called host range/helper function (hr/hf) mutants because they form plaques at 37 degrees C on BSC-1 and Vero monkey kidney cell lines but not on CV-1p monkey kidney cells. Wild-type SV40 can provide a helper function to permit growth of human adenoviruses in monkey kidney cells; the hr/hf mutants cannot. Progeny yields of hr/hf mutants are also cold sensitive in all cell lines tested. Patterns of viral macromolecular synthesis in three cell lines (Vero, BSC-1, and CV-1) at three …

Complex Motor Dynamics And Control In Multi-Looped Negative Feedback Systems, Anne Beuter, John Milton, Christiane Labrie, Leon Glass

WM Keck Science Faculty Papers

Experiments were performed in normal and Parkinsonian subjects who were asked to maintain a constant finger position using time-delayed visual feedback. The finger position showed complex dynamics, with characteristic differences between normal and Parkinosonian subjects. Here we discuss some of the theoretical issues that must be resolved in order to understand the mechanisms that underlie the generation of these complex signals.

Linker Insertion Mutants Of Simian Virus 40 Large T Antigen That Show Trans-Dominant Interference With Wild-Type Large T Antigen Map To Multiple Sites Within The T-Antigen Gene., Jiyue Y. Zhu, Charles N. Cole

Dartmouth Scholarship

Linker insertion mutants affecting the simian virus 40 (SV40) large tumor (T) antigen were constructed by inserting a 12-base-pair oligonucleotide linker into restriction endonuclease cleavage sites located within the early region of SV40. One mutant, with the insertion at amino acid 5, was viable in CV-1p and BSC-1 cells, indicating that sequences very close to the amino terminus of large T could be altered without affecting the lytic infection cycle of SV40. All other mutants affecting large T were not viable. In complementation assays between the linker insertion mutants and either a late-gene mutant, dlBC865, or a host range/helper function …

Significance Of Premature Stop Codons In Env Of Simian Immunodeficiency Virus, Toshiaki Kodama, Dawn P. Wooley, Yathirajulu M. Naidu, Harry W. Kestler Iii, Muthiah D. Daniel, Yen Li, Ronald C. Desrosiers

Neuroscience, Cell Biology & Physiology Faculty Publications

The location of the translational termination codon for the transmembrane protein (TMP) varies in three infectious molecular clones of simian immunodeficiency virus from macaques (SIV_mac). The SIV_mac251 and SIV_mac142 infectious clones have premature stop signals that differ in location by one codon; transfection of these DNAs into human HUT-78 cells yielded virus with a truncated TMP (28 to 30 kilodaltons [kDa]). The SIV_mac239 infectious clone does not have a premature stop codon in its TMP-coding region. Transfection of HUT-78 cells with this clone initially yielded virus with a full-length TMP (41 kDa). …

1989 Scholars And Artists Bibliography, Michael Schwartz Library, Cleveland State University, Friends Of The Michael Schwartz Library

Scholars and Artists Bibliographies

This bibliography was created for the annual Friends of the Michael Schwartz Library Scholars and Artists Reception, recognizing scholarly and creative achievements of Cleveland State University faculty, staff and emeriti

Embryonic Chicken Fibroblast Collagen Binding Proteins: Distribution, Role In Substratum Adhesion, And Relationship To Integrins, Roy C. Ogle, A. Jeannette Potts, Marchall Yacoe, Charles D. Little

Medical Diagnostics & Translational Sciences Faculty Publications

Collagen binding proteins (CBP) are hydrophobic, cell surface polypeptides, isolated by collagen affinity chromatography. Antibodies to CBPs inhibit the attachment of embryonic chicken heart fibroblasts to native type I collagen fibrils in a dose-dependent manner. The CBP antibodies also induce rounding and detachment of cells adherent to a planar substratum. This process of antibody-mediated substratum detachment resulted in a clustering of CBP and cell-associated extracellular matrix at the cell surface, and the rearrangement of filamentous actin. Other functional studies showed that cells grown within a three-dimensional gel of type I collagen cannot be immunostained at the cell surface with CBP …

Micronuclear Genome Organization In Euplotes Crassus: A Transposonlike Element Is Removed During Macronuclear Development, Scott Everet Baird, Gina M. Fino, Susan L. Tausta, Lawrence A. Klobutcher

Biological Sciences Faculty Publications

After mating, hypotrichous ciliated protozoa transform a set of their micronuclear chromosomes into thousands of short, linear DNA molecules that form the macronuclear genome. To examine micronuclear genome organization in the hypotrich Euplotes crassus, we have analyzed two cloned segments of micronuclear DNA as well as the macronuclear DNA molecules that are derived from them. E. crassus was found to display a number of features characteristic of other hypotrich genomes, including (i) clustering and close spacing of the precursors of macronuclear DNA molecules, (ii) the frequent occurrence of internal eliminated sequences within macronuclear precursors, (iii) overlapping macronuclear precursors, (iv) lack …

Chromosomal Localization Of Nucleic Acid-Binding Proteins By Affinity Mapping: Assignment Of The Ire-Binding Protein Gene To Human Chromosome 9, Matthias W. Hentze, Hector Seuanez, Stephen J. O'Brien, Joe B. Harford, Richard D. Klausner

Biology Faculty Articles

Three human mRNAs are regulated post-transcriptionally by iron via iron-responsive elements (IREs) contained in each mRNA. A cytoplasmic protein (IRE-BP) binds to these cis-acting elements and mediates the translational regulation of ferritin H- and L-chain mRNA and the iron-dependent stability of transferrin receptor (TfR) mRNA. We have taken advantage of the different mobilities of the human and rodent IRE/IRE-BP complexes on non-denaturing polyacrylamide gels to determine the chromosomal localization of the gene encoding the IRE-BP. Utilizing a panel of 34 different human/rodent hybrid cell lines we have assigned the IRE-BP gene to human chromosome 9. This new technique based on …

Collagen Binding Proteins Derived From The Embryonic Fibroblast Cell Surface Recognize Arginine-Glycine-Aspartic Acid, Roy C. Ogle, Charles D. Little

Medical Diagnostics & Translational Sciences Faculty Publications

Several cell surface proteins (Mr = 120,000, 90,000, 63,000 and 47,000) apparently integral to embryonic fibroblast plasma membranes were extracted with detergent and isolated by collagen affinity chromatography. Certain of these proteins (Mr = 120,000, 90,000, and 47,000) were specifically eluted from collagen affinity columns by synthetic peptides containing the amino acid sequence arginyl-glycyl-aspartic acid (RGD). These data show that a number of collagen binding proteins exist on the embryonic fibroblast cell surface. Some of the proteins may be collagen receptors binding to RGD sequences in the collagen molecule while at least one of the proteins (Mr = 63,000) recognizes …

The T(11;14)(P15;Q11) In A T-Cell Acute Lymphoblastic Leukemia Cell Line Activates Multiple Transcripts, Including Ttg-1, A Gene Encoding A Potential Zinc Finger Protein, Elizabeth A. Mcguire, Richard D. Hockett, Karen M. Pollock, Marty F. Bartholdi, Stephen J. O'Brien, Stanley J. Korsmeyer

Biology Faculty Articles

Interchromosomal translocations within lymphoid neoplasms frequently involve the antigen receptor genes. We cloned the breakpoints of the t(11;14)(p15;q11) in a CD3-negative T-cell acute lymphoblastic leukemia cell line (RPMI 8402) in order to identify new genes potentially involved in T-cell neoplasia. An extensive comparison of both breakpoints and their germ line counterparts indicated that an inadvertant recombinase-mediated break at chromosome segment 11p15 recombined with the delta T-cell receptor at 14q11. The derivative 11 breakpoint resembles a coding joint in which 11p15 rather than a variable region was introduced 5' to a D_δ1D_δ2J_δ1 intermediate rearrangement. Conversely, the …

Specificity Of The Cardiac Response To Conspecific Vocalizations In Chimpanzees, Gary G. Berntson, Sarah T. Boysen

Sentience Collection

Heart rate measures were used to examine the functional response of young chimpanzees and orangutans to acoustic stimuli, including white noise and chimpanzee vocalizations (threat, stress, and alarm). The initial response of the animals to all stimuli was characterized by a prominent cardiac deceleration and an increase in heart period variability. The deceleratory responses persisted with repeated presentations of the noise, stress, and alarm stimuli. In contrast, the response of chimpanzees to the conspecific threat stimulus reverted over trials to a notable cardiac acceleration. This acceleratory response was not attributable to potential patterns of evoked somatic responses. The features of …

Effects Of Age On Motor Preparation And Restructuring, Paul Amrhein, Noreen Goggin, George Stelmach

Department of Psychology Faculty Scholarship and Creative Works

Age-related decrements in motor plan restructuring were investigated. In this experiment, older and younger adults performed a discrete aiming task that involved responses that were precued and responses that were modified at the time of an imperative signal. On 75% of the trials, the precue specified the response-stimulus (valid trials) with respect to the movement parameters of the arm (left or right) and direction (toward or away). On the remaining 25% of the trials, the response-stimulus was different from the precue (invalid trials) in that the subject was required to modify a planned movement by changing the arm to be …

Explorations, Vol. 5, No. 2, Kathleen Lignell, Mary Beth Pinto, Susan White, James M. Acheson, Mary Lee, John Field, Eric Beenfeldt, Robert Shepard, M. W. Blumenstock

Explorations — A Journal of Research

Articles include:

Cover: Brian McLain of New Harbor, measuring a V-notched lobster; slide taken by Robert C. Bayer, University of Maine Professor of Animal and Veterinary Sciences.

"April is Aquaculture Month Guest Editorial," by Kathleen Lignell

"Hospital Project Teams: Gaining Cooperation," by Mary Beth Pinto

"The Maine Lobster Institute: Science and Industry Working Together," by Susan White

"The Theory of Common Property Resources: Scientific Law or Myth?" by James M. Acheson

"What do Seedless Watermelons and Triploid Oysters have in Common?" by Mary Lee

"Technology and the Competitive Edge," by John Field and Eric Beenfeldt

"Growing our State Tree Faster: …

Replicated Single Row Evaluation., C. M. Saunders

Experimental Summaries - Plant Research

Trial 89AB12

Great Southern Agricultural Institute - Paddock B15

This trial was of similar design to the single row evaluation, except four replications and pesticide treatments were also included.
However the trial had to be abandoned due to severe phytotoxic responses seen in the medics due to repeated spraying of 'Lorsban' (used at recommended rates).
Intended to repeat this work in 1990, bulk seed where possible and to eventually assess these lines, under such conditions, in large plots.

Acceptance Of Simulated Oral Rabies Vaccine Baits By Urban Raccoons, John Hadidian, Suzanne R. Jenkins, David H. Johnston, Peter J. Savarie, Victor F. Nettles, David M. Manski, George M. Baer

Veterinary Science and Medicine Collection

In summer 1986, a study was conducted to evaluate raccoon (Procyon lotor) acceptance of oral baits that could be used for rabies vaccination, One thousand wax-coated sponge bait cubes were filled with 5 mg of a seromarker (iophenoxic acid), placed in polyethylene bags, and hand-distributed in an 80 ha area within an urban National Park in Washington, D.C. (USA), After 3 wk, target and nontarget animals were trapped and blood samples collected to evaluate bait uptake. Thirty-three of 52 (63%) raccoons had elevated blood iodine levels indicating they had eaten at least one bait, 13 (25%) were negative, and six …

Characterization Of Chromosome Fragmentation In Two Protozoans And Identification Of A Candidate Fragmentation Sequence In Euplotes Crassus, Scott Everet Baird, Lawrence A. Klobutcher

Biological Sciences Faculty Publications

Following the sexual cycle, hypotrichous ciliated protozoans fragment a set of their micronuclear chromosomes to generate the thousands of short, linear DNA molecules present in the transcriptionally active macronucleus. We have used a hybrid selection procedure to examine macronuclear DNA molecules for subtelomeric length heterogeneity to determine whether chromosome fragmentation occurs at unique or multiple sites. The results suggest that multiple, but closely spaced, chromosome fragmentation sites are used by Oxytricha nova. In contrast, Euplotes crassus uses unique chromosome fragmentation sites in a reproducible manner to generate the ends of macronuclear DNA molecules. Additional studies compared DNA sequences in …

Ua35/11 Student Honors Research Bulletin, Wku Honors Program

WKU Archives Records

The WKU Student Honors Research Bulletin is dedicated to scholarly involvement and student research. These papers are representative of work done by students from throughout the university.

• Coates, Alan. Management's Attitude Toward Collective Bargaining
• Frew, Becky. The Effects of Personality Type and Social Support on Instances of Job Stress
• Green, Laura. Earth Sheltered Housing
• Gregory, Vicky. Fraud and Abuse by Medical Providers in Medicare and Medicaid
• Hignite, Susan. Japanese Students in Kentucky Classrooms
• Hodum, Martha. The Mathematically Gifted Child
• Jones, Rodney. Superman to Censorship: America's Romance with the Comic Book
• Marrs, Shawn. Romanticism and Unromanticism in Sir Gawain and the …

From The Gulf To The Rio Grande: Human Adaptation In Central, South, And Lower Pecos Texas, Thomas Hester, Stephen L. Black, D. Gentry Steele, Ben W. Olive, Anne A. Fox, Karl Reinhard, Leland C. Bement

Karl Reinhard Publications

The South Texas area, Region 3 of the Southwestern Division, U.S. Army Corps of Engineers, is synthesized from archeological and bioarcheological perspectives. Three distinct geographic units within Region 3 are treated in detail: Central Texas Plateau Prairie, South Texas Plains, and Lower Pecos Canyonlands. More than 11,000 years of human adaptation are chronicled for this area, stretching from the Gulf of Mexico to the Rio Grande along the border with northeastern Mexico. Particular attention is devoted to a consideration of the region's prehistoric record; significant problems and data gaps are outlined. For the first time, a compilation has been done …

A Mimbres Burial With Associated Colon Remains From The Nan Ranch Ruin, New Mexico, Harry J. Shafer, Marrianne Marek, Karl J. Reinhard

Karl Reinhard Publications

The skeletal remains of an adult male associated with desiccated tissue and a coprolite were recovered from an apen-air midden deposit at the NAN Ranch Ruin (LA15049)) a large Mimbres site in Grant County) New Mexico. The find dates to about A.C. 1000-1100. Identifiable macroscopic elements in the caprolite consist offi nely fragmented corn and tiny seed fragments of an unknown plant. High amounts of willow (Salix) and mustard (Brassicaceae) pollen may indicate the ingestion of medicinal plants to combat a deteriorating health condition. The individual was approximately 35 -40 years old at the time of death and suffered from …

Bioarcheological Synthesis For "From The Gulf To The Rio Grande: Human Adaptation In Central, South, And Lower Pecos Texas", Karl Reinhard, Ben W. Olive, D. Gentry Steele

Karl Reinhard Publications

One of the main problems encountered in the review of the bioarcheology of Region 3 has been the limited number of sites where human skeletal material has been adequately recovered and analyzed. In the preceding chapter it was documented that less than 30% of the burials recovered from recorded sites have been reported in published literature. It was further estimated that of the 323 sites with burials, no more than 80 sites have published detailed bioarcheological reports on the burials recovered. Only 50 of these 80 reports provide individual descriptions of each burial which facilitate subsequent analyses and evaluation.

Four …

The Amino Acid Sequence Of The Adult Sumatran Tiger (Panthera Tigris, Carnivora) Hemoglobins, Meeno Jahan, Aftab Ahmed, Gerhard Braunitzer, Reinhard Göltenboth

Pharmacy Faculty Articles and Research

The complete amino-acid sequences of the hemoglobins from the adult Sumatran tiger (Panthera tigris sumatrae) have been determined on automatic liquid- and gas-phase sequenators. The globin chains were isolated by reverse phase HPLC on a column of Nucleosil-C4.7V-Acetylserine was detected by FAB-mass spectroscopy as TV-terminal amino acid residue of the βI chain. Comparing the sequences of the globin chains of the tiger with that of human Hb-A, 23 substitutions were recognized in the a, 29 in βI and 28 in the βII chain.

Serradella Evaluations, L. Cransberg

Experimental Summaries - Plant Research

Trial 85AL56

Location: South Sterlings.

Serradella line evaluation - Stage 3.

To evaluate five promising lines selected from variety trials, in terms of herbage production and long term persistence.

Trial 88AL32

Location: Walpole.
Long season serradella evaluation.

To select productive and persistent serradella lines for the deep acidic sands in the very high rainfall zone on the south coast.

Trial 88AL31

Location: Takalarup.

Ryegrass species evaluation.

To investigate the potential of a range of ryegrasses, from short term to long term perennial, in the south coast environment.

Grass species evaluation

Location: Greenrange.

To identify both productive and persistent grasses for …

Case Report Of Human Infection With Capillaria Philippinensis, Ching-Yu Chen, Wei-Chuan Hsieh, Tsang-Lie Chen

Harold W. Manter Laboratory: Library Materials

In July 1988, a case of human infection with Capillaria philippinensis, so-called the intestinal capillariasis, was discovered at the National Taiwan University Hospital (NTUH). This is the first case reported in Taiwan.

Capillaria philippinesis is a parasite of fish-eating birds In nature it has a fish-bird life cycle. When human populations disrupt the natural cycle by eating raw fish infected with the larvae of C. philippinensis, they may acquire the disease.