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Transgenic Basmati Rice Transformed With The Xa21 Gene Shows Resistance Against Bacterial Leaf Blight, Zahid Mukhtar, Shahida Hasnain
Transgenic Basmati Rice Transformed With The Xa21 Gene Shows Resistance Against Bacterial Leaf Blight, Zahid Mukhtar, Shahida Hasnain
Turkish Journal of Botany
Rice (Oryza sativa L.) cultivar Super Basmati was transformed with a cloned gene, Xa21, through particle bombardment method. Twenty-two plants were recovered from the selected calli. Initial screening through PCR confirmed the presence of the transgene in 21 plants. Southern analysis further confirmed the integration of the Xa21 gene into the genome of these transgenic plants. Analysis of the T1 generation revealed that both the hpt and Xa21 genes were successfully transmitted from the primary transformants to the progeny. Bioassays with two local isolates of bacterial leaf blight pathogen, Xanthomonas oryzae pv. oryzae (Xoo), showed that 14 transgenic lines were …