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Diversity Of Cultured Bacteria From The Perennial Ice Block Of Scarisoara Ice Cave, Romania, Corina Iţcuş, Mădălina-Denisa Pascu, Traian Brad, Aurel Perşoiu, Cristina Purcarea
Diversity Of Cultured Bacteria From The Perennial Ice Block Of Scarisoara Ice Cave, Romania, Corina Iţcuş, Mădălina-Denisa Pascu, Traian Brad, Aurel Perşoiu, Cristina Purcarea
International Journal of Speleology
Cave ice ecosystems represent a poorly investigated glacial environment. Diversity of cave ice bacteria and their distribution in perennial ice deposits of this underground glacial habitat could constitute a proxy for microbial response to climatic and environmental changes. Scarisoara Ice Cave (Romania) hosts one of the oldest and largest cave ice blocks worldwide. Here we report on cultured microbial diversity of recent, 400, and 900 years-old perennial ice from this cave, representing the first characterization of a chronological distribution of cave-ice bacteria. Total cell density measured by SYBR Green I epifluorescence microscopy varied in the 2.4 x 104 – …