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Characterization And Identification Of A Sludge-Associated Bacterial Isolate, Abiodun O. Adibi, Derrell Mcpherson, Minna Laine
Characterization And Identification Of A Sludge-Associated Bacterial Isolate, Abiodun O. Adibi, Derrell Mcpherson, Minna Laine
Virginia Journal of Science
During a metal speciation study, an unusually high retention of lead ·was observed when lead solution was percolated through a column packed with sludge compost obtained from Hampton Roads Sanitation department. A bacterium was isolated from the sludge compost and identified as Bacillus sphaericus using electron microscopy, whole cell fatty acid analysis (Midi System) and Biolog GP Microplate. The isolate grows in broth and agar media containing up to 800 µM lead. Lead accumulation study using atomic absorption spectrophotometer indicates that the isolate adsorbs lead. Lead adsorption is pH dependent. The isolate contains a plasmid of approximately 40 -50 kbp …