Life Sciences Commons^™

Community-Scale Water Treatment Systems In The Dominican Republic, Jonathan Racey, Annabelle Papai, Elise Fischer, Becca Johnson

Engagement & Service-Learning Summit

Engagement and Service-Learning Summit: Reciprocal and Sustainable Partnerships

Prenylation In The Moss Physcomitrella Patens, Noela Botaka, Susana Perez-Martinez, Liang Bao, Parul Singh, Mark Running

Posters-at-the-Capitol

Protein prenylation is a post-translational modification that involves the addition of lipid groups to the end of a target protein and is necessary for protein activity. Prenylation has important roles in the cell: targeting and localizing proteins to subcellular compartments and promoting protein-protein interactions. Recently, we have found Protein Prenyltransferase Alpha Subunit-like (PPAL), which shares structural similarities to known prenylation enzymes. However, the biochemical function of PPAL is still unknown. PPAL is present in a single copy in other plants examined to date but is present in two copies in moss. Knockouts in our lab of either PpPAL1 or PpPAL2 …

A Crispr Platform For Rapid And Inducible Genome Editing In Human Non-Small Cell Lung Cancer Cells, Lloyd Bartley

Posters-at-the-Capitol

Non-small cell lung cancer (NSCLC) accounts for about 85% of lung cancer, which is the leading cause of cancer death in the world. High mortality rate associated with NSCLC is partially attributed to the limited understanding of NSCLC as well as ineffective therapeutic treatments. The initiation and progression of NSCLC involves genetic changes leading to alterations in the control of tissue development and homeostatic maintenance. Better knowledge about these genetic abnormalities is imperative for developing new chemotherapeutic drugs for NSCLC. Recent research demonstrates that the expression of paraoxonase 2 (PON2), a lactonase/arylesterase with anti-oxidant properties, are markedly enhanced in cancer …

Characterization Of A Basement Membrane Associated Protein Encoding Gene In Drosophila Melanogaster, Aref Ranjbar, Ajay Srivastava

Posters-at-the-Capitol

Title: Characterization of a Basement Membrane Associated Protein Encoding Gene in Drosophila melanogaster

Authors: Aref Ranjbar, Mayank Kapadia, Ajay Srivastava, PhD(faculty member, mentor)

Basement Membranes (BM) are important for normal development and tumor progression. In order to get a better understanding of BM dynamics we identified genes that encoded BM interacting proteins. One such gene is predicted to be involved in vesicle-mediated transport in Drosophila melanogaster. Here we characterize this gene by utilizing molecular biology techniques like immunohistochemistry, RNA in situ hybridization, and Western blot analysis utilizing antibodies generated in the laboratory. Western blot analysis identified this protein to be …

Novel Inactivation Of The Causative Fungus Of White Nose Syndrome With Methoxsalen And Ultraviolet A Light, Colin J. Hartman, Joseph Mester Phd, Alan Cohen Md

Posters-at-the-Capitol

White Nose Syndrome (WNS) is a newly recognized disease responsible for the rapid mass destruction of the North American bat populations. This study addressed the novel inactivation of fungal spores from Pseudogymnoascus destructans, the causative agent of WNS, using ultraviolet A (UVA) light at 365nm and methoxsalen, a photosensitizer from a family of compounds known as furanocoumarins. Penicillium crustosum, an environmental fungus, was studied as a comparator.

Spore suspensions were soaked in specific concentrations of methoxsalen and subsequently exposed to UVA light. The plates were examined for both spore inactivation and resultant inhibition of colony growth. The results demonstrated …

Discovery Of Unreported Sponge Taxa, With Potential As Species New To Science, From The Chicago Area, Jennifer E. Slate

Faculty Research and Creative Activities Symposium

No abstract provided.

25 Years Of Battling Academic Hegemony, Gender Stereotypes And Political Power: Deconstructing The Mantis Visual System In The Service Of Helping Children, Frederick R. Prete

Faculty Research and Creative Activities Symposium

No abstract provided.

Courtship Behavior, Communication, And Copulation In Tigrosa Annexa, Samuel White

Scholars Week

The evolution of multimodal communication, where signalers use multiple signal components in multiple sensory modalities, has become the subject of investigation by many researchers. Signaling puts males at risk of predation, so why do males of some species evolve extra signals that may increase this risk? In some wolf spider species, males incorporate many visual and vibrational signals into a display that they use to attract a female for mating. Female spiders are often aggressive toward courting males and so the male display also functions to decrease the odds of cannibalism. Female wandering spiders deposit silk containing pheromones that communicate …

Modeling Werner Syndrome In Drosophila, Elyse A. Bolterstein, Aaron E. Schirmer

Faculty Research and Creative Activities Symposium

No abstract provided.

Examining Delayed Onset Of Dementia In The Bilingual Geriatric Population, Erica Brown, Elizabeth Hartman

Grace Peterson Nursing Research Colloquium

Background: Dementia is the largest cause of dependency and disability in older adults, affecting nearly 50 million people worldwide with about 10 million new cases every year. Presently, there are no cures for dementia. Consequently, a growing body of evidence suggests that bilingualism may delay the onset of clinical dementia symptoms by several years.

Objectives: The purpose of this review is to summarize and analyze current evidence from studies that examined how bilingualism delays the onset of dementia. Evidence is reviewed suggesting that bilingualism may delay the dementia symptoms due to an increase in cognitive reserve, which refers to an …

Metabolic Processing By The Mtorc1 Pathway In The Zebrafish Yolk Cell, Jorge A. Cantu

Faculty Research and Creative Activities Symposium

No abstract provided.

The Development Of Hybrid Lipid-Polymer Nanoparticle Architectures For The Sustained-Release Of Small Hydrophilic Molecules, Keegan Curry, Jill M. Steinbach-Rankins Dr.

Posters-at-the-Capitol

Introduction: Polymeric nanoparticles (NPs) have been utilized as drug delivery vehicles for a variety of applications. However, achieving sustained-release of small hydrophilic agents is a primary challenge for their use in prolonged delivery applications.

Objective: This study investigates how novel lipid-polymer hybrid particle architectures can be used to improve the release profile of small hydrophilic encapsulants. Here, PLGA NPs were produced via electrospraying and emulsions. Particles with a core-shell architecture were produced via coaxial electrospraying and the ability of this architecture to sustain release was examined. In addition, we combined polymeric core-shell NPs with a lipid coating to improve biocompatibility, …

Soil Responses To One-Season Winter Cover Crops, Mckenna Morgan, Iin Handayani

Posters-at-the-Capitol

Soil Responses to One-Season Winter Cover Crops

McKenna Morgan and I. P. Handayani

Hutson School of Agriculture Murray State University, KY, USA

Abstract

In regions like western Kentucky where wooded lots are common there is a growing interest in how to utilize them so that land profits can be maximized. The frequently poor soils in wooded areas due to erosion and lack of ground cover vegetation often cause complications in the creation of pasture areas. Therefore, the objective of this study was to assess the effect of growing winter cover crops on soils to prepare for pasture systems in the …

Utilizing Municipal Compost And Equine Stall Waste As Potential Economic Alternatives In An In-Ground Pot-In-Pot Production System, Ashley Robert, Alyx Shultz

Posters-at-the-Capitol

For Western Kentucky farmers, one viable income source could be a pot-in-pot nursery production. One of the highest costs of production in this system was the planting media that the plants were grown in. Economical alternatives to high-priced, non-renewable peat-based mixes were important to consider. Unique to this region, were two renewable soil amendments that may help farmers to widen their profit margin in a pot-in-pot system. This research looked at the economic viability of locally sourced horse stall waste and municipal compost as soil amendments to a traditional bark and peat based mix. Initial cost projections were favorable for …

Consumption Profiles Of Illicit And Neuropsychiatric Drugs In Urban And Rural Communities In Kentucky Using Sewage Epidemiology, Tara Croft, Katelyn Foppe, Rhiannon Huffines, Bikram Subedi

Posters-at-the-Capitol

The drug overdose deaths in the USA increased by ~20% from 2015 to 2016 to the total >63,600 drug overdose deaths in 2016. Kentucky is among the highest rate of drug overdose death states in the USA. The current estimates of the prevalence of substance abuse are based on the self-reported surveys, overdose/toxicological reports, and drug-related crime statistics. Survey-based conventional approaches are not only cost and time-intensive but also underestimate the actual consumption of drugs. As “wastewater never lie,” the drug residues in raw wastewater collected from the centralized wastewater treatment plants were utilized to determine the consumption rate of …

Impact Of Aerobic Compost Tea On Growth Of Lactuca Sativa Within A Peat-Based Growing Media, Ella Potts, Jessica Stoner, Alyx Shultz, Iin Handayani

Posters-at-the-Capitol

Compost and compost tea have been shown in practice to be valuable soil amendments across many horticultural applications; however, little rigorous quantitative work has been completed. Researchers designed a side-by-side trial with two replications to quantifiably study the impact of compost tea on the growth and vigor of soil-grown lettuce. A recycled poultry-litter-based aerobic compost tea was used for the treatment. Six lactuca sativa plants received each treatment or control. Researchers hypothesized the treatment plants would show more vigor and vegetative growth than control. The null hypothesis was treatment plants would be identical to control lettuce. Researchers failed to reject …

Development Of A Pd-L1 Pet Imaging Biomarker, Caleb Jack Bridgwater

Posters-at-the-Capitol

Immunotherapy strategies are very promising treatments for cancer patients. Specifically, Immune checkpoint inhibitor therapy focusing on the PD-1/PD-L1 pathway shows long-lasting positive results in many cancer patients. Unfortunately, not all the patients can benefit from this highly effective treatment. Hence, there is a great need for predictive biomarkers. Immunohistochemical (IHC) staining has been used as a way of predicting patient response, yet shows many problems. For example, IHC utilizes an invasive biopsy and sample fixing, which creates an incomplete and delayed picture of the patient’s biochemistry and the tumor microenvironment, consequently ignoring metastases.

The purpose of this study is to …

How Does Antibiotic Resistance Spread In Tuberculosis?, Julie A. Spencer

Shared Knowledge Conference

The ancient bacterial disease of tuberculosis (TB) is curable with antibiotics, but according to the World Health Organization, in 2016 over 10 million people became infected with the disease. 600,000 of these cases were resistant to antibiotics, yet the worldwide treatment success rate for drug resistant TB is only 54%. Furthermore, strains exist now that are resistant to all known antibiotics. In the current environment of global travel, this poses a risk for emergent epidemics of drug resistant TB. In this study, I asked: after an antibiotic resistant strain has evolved in someone’s lungs, how does it spread? It is …

Ponderosa Pine Seedling Physiology After Severe Wildfire: Does It Take A Forest To Make A Forest?, Lauren Bansbach

Shared Knowledge Conference

Ponderosa pine forests are an iconic ecosystem of western North America and provide numerous ecological, economic, and cultural benefits. Historically, these forests experienced regular surface fires that left most large, mature trees surviving. However, suppression of wildfires along with a warming climate have led to catastrophic tree-killing fires that result in vast areas (>200 mi2) dominated by shrubs and grasses. Because of limited survival of tree seedlings in these post-fire habitats, it remains unknown whether ponderosa pines can successfully recolonize severely burned landscapes. Without a mature tree canopy to provide periodic shade, seedlings experience hotter, drier conditions and likely …

The Paradoxical Giant Hummingbird: Comparison Of Andean And Coastal Subspecies With Respect To Blood, Migration, And Genes, Jessie L. Williamson

Shared Knowledge Conference

The Giant Hummingbird (Patagona gigas) is twice as large as the next largest hummingbird species and has long been considered paradoxical with respect to flight biomechanics. It is also an extreme outlier in other respects. For example, it is the only hummingbird species that breeds above 4,000 m elevation and also along the beaches of the Pacific Ocean. The high Andean populations of Giant Hummingbird (P. g. peruviana) that we have studied previously have a beta-hemoglobin genotype (serine at beta-hemoglobin A positions 13 and 83) that is characterized by high O2-affinity and is only shared with four unrelated hummingbird taxa …

Assessing Water Policy Implications Of The Changing Agriculture In New Mexico, Trevor A. Birt

Shared Knowledge Conference

Agriculture is the dominant water sector in New Mexico, responsible for more than 80% of water withdrawals in 2015 (USGS 2018). Water policy needs to be extremely adaptive and informed to meet the needs of not only farmers, but cities, industry and riparian uses. Since 1840, the U.S. Department of Agriculture (USDA) has conducted census surveys assessing all levels of agriculture. These data are physically published at the county, state, and national level on a 5-year cycle, detailing various material counts such as acreage, yields, and water usage, as well as socioeconomic estimates of farm costs, revenues, and hired workers. …

Rainfall Changes Alter Plant Communities In Chihuahuan Desert Grasslands, Timothy Ohlert

Shared Knowledge Conference

Variations in New Mexico’s climate have been detected over the past century and are expected to continue into the future. Some of these climatic changes result in more frequent instances of extreme drought events and alterations of monsoon seasonality, with monsoons being pushed later into the fall. Such changes could make our Chihuahuan Desert plant communities less productive, shift the abundance of the plant species, or have other destabilizing impacts. Our experiment manipulated precipitation in two desert grassland communities at the Sevilleta National Wildlife Refuge near Socorro, NM. Some plots were subjected to an extreme drought event, receiving only 33% …

Engage A Voice, Repress Fatigue; The Coincident Evolution Of Hominin Vocalization And A Metabolic Threshold, Galen A. Morton 9935238, Martin L. Morton

Shared Knowledge Conference

Physiologically self-protective mechanisms borne from hominin evolutionary history that increase survivability are not unknown to science. In reviewing exercise science literature regarding testing and assessment measures of subjects talking while exercising, a logical question has materialized: To what degree has evolution facilitated synchronization of comfortable oral communication with sustainable exercise intensity? An individual able to engage a voice, represses fatigue. The Talk Test, is a practical strategy whereby a subject deliberately speaks during an exercise protocol. It is a common tool in both kinesiology and clinical fields because it inherently identifies a pivotal metabolic threshold. The coincidence of comfortable ability …

Combined High-Speed Single Particle Tracking Of Membrane Proteins And Super-Resolution Of Membrane-Associated Structures, Hanieh Mazloom Farsibaf, Keith A. Lidke

Shared Knowledge Conference

Many experiments have shown that the diffusive motion of lipids and membrane proteins are slower on the cell surface than those in artificial lipid bilayers or blebs. One hypothesis that may partially explain this mystery is the effect of the cytoskeleton structures on the protein dynamics. A model proposed by Kusumi [1] is the Fence-Picket Model which describes the cell membrane as a set of compartment regions, each ~ 10 to 200 nm in size, created by direct or indirect interaction of lipids and proteins with actin filaments just below the membrane. To test this hypothesis, we have assembled a …

Temporal Information Guides Prefrontal Preparatory Activity, Jacqueline R. Janowich

Shared Knowledge Conference

Proactive preparation for an upcoming goal differs from last-minute reactive adaptation, but it is unclear how preparatory mechanisms change based on when in the future a goal needs to be executed. To assess how timing information is integrated into preparatory control, we designed a novel variant of the Dot Pattern Expectancy task, where each cue signaled both task rule and delay duration (known short, known long, or unknown) between cue and probe. We recorded EEG while healthy young adult participants (n=36) performed this task, and found that delay demands elicited distinct prefrontal preparatory activities. Medial prefrontal amplitude was sensitive to …

Mechanisms For The Spread Of Antibiotic Resistance In Tuberculosis, Julie Allison Spencer

Shared Knowledge Conference

Tuberculosis (TB) is currently the ninth leading cause of death for humans worldwide, causing 10.4 million new infections in 2016. According to the World Health Organization, of these infections, 600,000 were antibiotic resistant. However, the treatment success rate for resistant TB was only 54%. The potential for emergent epidemics of drug resistant TB highlights the need to understand the mechanisms for the spread of resistance. Mycobacterium tuberculosis, the bacterium that causes TB, evolves resistant strains within individuals who are being treated with antibiotics. It has been assumed previously that the resistant characteristics of these strains are spread primarily by the …

The Critical Need For Mental Health Education To Be Mandated In New Mexico's Public Schools, Bonnie L. Murphy

Shared Knowledge Conference

Based on a review of research and best practices in mental health awareness and skills, this inquiry project argues for state legislative policies that would require mental health awareness and skills in the K-12 curriculum. Mental health affects individual accomplishments in every stage of people’s lives beginning in early childhood and throughout the life cycle. Prevention and treatment of mental illness plays a key role in the ability of an individual to cope with loss and develop resiliency and perseverance in challenging times and to make better decisions that improve the individual’s life and the lives of those around them. …

Histone Deacetylation Is The Primary Epigenetic Mechanism For Silencing Of Tumor Suppressor Gene - Tissue Factor Pathway Inhibitor-2 In Hepatocellular Carcinoma Cells, Alisha Deshmukh, Shirish Barve

Posters-at-the-Capitol

Hepatocellular carcinoma (HCC) is the third leading cause of cancer related mortality worldwide. With a survival rate of less than 5 percent, a therapeutic treatment is desperately needed to manage this disease. Many epigenetic mechanisms that underlay HCC are being identified. A frequently silenced pathway tissue factor pathway inhibitor-2 (TFPI-2) is a critical tumor suppressor gene. In HCC, inactivation of TFPI-2 leads to tumor growth. Recent research indicated Fas L plays a major role in apoptosis as part of HCC. For the purposes of this study, the phytochemical Curcumin was explored to observe its possible effects on the epigenetic mechanisms …

Preface, Weixing Song

Conference on Applied Statistics in Agriculture

Preface

Cleveland Clinic Florida - Summer Scholar Program, Marina Handal

Trick to the Treat of Internships and Research

Program Overview:

• Cleveland Clinic Florida’s Summer Scholar Program is designed to allow students to gain shadowing experience in the medical field via the clinical and hospital settings.
• Students are assigned 3 rotations total within the 6-week span of the program; high school assignments run for a total of 3 weeks.
• Rotations include Internal Medicine, Cardiology, Neurosurgery, ICC, Emergency Medicine, and various other subspecialties.
• Students can expect to be at the Weston hospital Monday-Friday 8 am- 5 pm (varies based on rotation/clinic hours/surgery schedule).
• Attend weekly lectures that consist of discussion of the ethics of medicine, educational path to becoming a …