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Full-Text Articles in Life Sciences
Salty Genes: Rapid Discovery Of Salt Tolerance Genes In The Bacteria Kushneria, Andrew Jenkins, Brent Nielson, Jonathon T. Hill
Salty Genes: Rapid Discovery Of Salt Tolerance Genes In The Bacteria Kushneria, Andrew Jenkins, Brent Nielson, Jonathon T. Hill
Library/Life Sciences Undergraduate Poster Competition 2023
1st place poster design
Global rising levels of soil salinity have led researchers to seek microbial solutions to increase agricultural plant viability in high salt soils.
Kushneria increases growth of inoculated alfalfa in high salt soils, but little is known about how Kushneria survives in these environments1 or how it can be used to reduce the economic burden of salted soil.
Expressing And Purifying Type Iv Crispr Accessory Proteins, Alivia Jolley
Expressing And Purifying Type Iv Crispr Accessory Proteins, Alivia Jolley
Fall Student Research Symposium 2022
CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)-Cas (CRISPR-associated) adaptive immune systems defend bacteria and archaea against phages, plasmids, and other mobile genetic elements. Each Type IV CRISPR system contains a subtype-specific gene and is hypothesized to be essential for function. The Type IV-A system encodes ATP-dependent 5'-3' DNA helicase called CasDinG, while the Type IV-B systems encode a putative pyrophosphatase named CasCysH. Here we investigate the function of the type IV CRISPR systems by expressing, purifying, and characterizing the accessory proteins from the type IV systems. This project is a first step towards understanding the structure and mechanism of type …
Characterization Of The Atpase Activity Of Casding, Christian Cahoon
Characterization Of The Atpase Activity Of Casding, Christian Cahoon
Fall Student Research Symposium 2021
The battle between bacteria and phage has been ongoing for eons. This battle has generated the evolutionary pressure necessary for the development of microbial immune systems. Characterization of these systems has led to the discovery of molecular tools such CRISPR-Cas systems. This system uses a genetic memory of past viral infections coupled with associated proteins to form ribonucleoprotein complexes which seek out and destroy foreign genetic elements. These systems have been repurposed by scientists to create powerful gene editing tools such as Cas9. With such powerful molecular tools being discovered, we have pursued the characterization of a relatively unknown system, …
Determining The Nucleic Acid Binding Affinities Of Crispr-Associated Ding (Casding), Matt Armbrust
Determining The Nucleic Acid Binding Affinities Of Crispr-Associated Ding (Casding), Matt Armbrust
Fall Student Research Symposium 2021
CRISPR-Cas systems are adaptive prokaryotic immune systems that enable host cells to defend against attack from foreign nucleic acids such as phage infections or plasmids. CRISPR-Cas systems are diverse and encompass 2 classes, 6 types, and 33 subtypes. The Type IV-A CRISPR-Cas system from Pseudomonas aeruginosa strain 83 is composed of five different genes (csf1, csf2, csf3, cas6, and dinG). Type IV-A systems are poorly understood, and currently there is little research detailing their biological and biochemical mechanism of immunity. CasDinG, an ancillary protein within the Type IV-A system, is required for an immune response in vivo. However, the role …
Discovering Virally Encoded Proteins That Block Type Iv Crispr Immune Systems, Andrew Williams
Discovering Virally Encoded Proteins That Block Type Iv Crispr Immune Systems, Andrew Williams
Fall Student Research Symposium 2021
Bacteria and the viruses that infect them have been at war from the beginnings of life until today. Due to selective pressure from viral infection, bacteria have evolved various biological defense systems, including CRISPR-Cas systems that use a genetic memory of previous viral encounters to protect against future invasions. However, recently it has been shown that viruses have evolved counter-strategies to evade CRISPR systems. Virally encoded proteins called anti-CRISPRs use a variety of mechanisms to block the activity of CRISPR immune systems in order to infect bacterial cells. The Jackson lab at USU recently showed that a Type IV-A CRISPR-Cas …