Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 4 of 4
Full-Text Articles in Life Sciences
Quantitative Shotgun Proteomic Analysis Of Bacteria After Overexpression Of Recombinant Spider Miniature Spidrion, Masp1, Kathryn P. Randene
Quantitative Shotgun Proteomic Analysis Of Bacteria After Overexpression Of Recombinant Spider Miniature Spidrion, Masp1, Kathryn P. Randene
University of the Pacific Theses and Dissertations
Spider silk has extraordinary mechanical properties, displaying high tensile strength, elasticity, and toughness. Given the high performance of natural fibers, one of the long-term goals of the silk community is to manufacture large-scale synthetic spider silk. This process requires vast quantities of recombinant proteins for wet-spinning applications. Attempts to synthesize large amounts of native size recombinant spidroins in diverse cell types have been unsuccessful. In these studies, we design and express recombinant miniature black widow (Latrodectus hesperus) MaSp1 spidroins in bacteria that incorporate the NTD and CTD, along with varying numbers of codon-optimized internal block repeats. Following spidroin overexpression, we …
Microbial Community Analysis: Biofilm Inhibition & Algae Associated Community Structure, Michelle V. Fong
Microbial Community Analysis: Biofilm Inhibition & Algae Associated Community Structure, Michelle V. Fong
University of the Pacific Theses and Dissertations
Natural products chemistry is the pursuit of bioactive small molecules from living organisms. These can be classified as primary metabolites if they are essential to survival, and secondary metabolites if they are accessory, playing a role in communication, defense, recruitment, etc.. Natural products have made a significant contribution to society – of 1,881 FDA-approved drugs from 1981 to 2019, 4% were pure natural products, 19% were natural products derived, and 3% were synthetic drugs with a natural products pharmacophore targeting a wide range of diseases and infections (Newman & Cragg, 2020). Pharmacophores are structural components of drugs that are responsible …
In Vitro Activity Of Four Fluoroquinolones On Selected Bacteria, Sheryl L. Pullen
In Vitro Activity Of Four Fluoroquinolones On Selected Bacteria, Sheryl L. Pullen
University of the Pacific Theses and Dissertations
In 1990-1991, in a national surveillance study, and in 1991-1992, in a followup study, both by Thornsberry et al. (1993), ciprofloxacin data from various geographical and demographical institutions were collected. Several species of bacteria have shown resistance to ciprofloxacin and norfloxacin, but the degree of resistance to these drugs has not been reported for the Stockton area. To determine the extent of this resistance, Dameron Hospital antibiograms generated from 1990 to 1994 were reviewed and compared. Results of the comparison show that susceptibility among the Gram-negative isolates, with the exception of Providencia stuartii, Acinetobacter lwoffi, and to a lesser extent …
Studies On An Antibiotic-Producing Strain Of Bacillus Megaterium X, Kishori Madhukar Chaubal
Studies On An Antibiotic-Producing Strain Of Bacillus Megaterium X, Kishori Madhukar Chaubal
University of the Pacific Theses and Dissertations
On July 10, 1968, while observing the effects of ultra-violet light on a plate culture of Sarcino lutea, o contaminant was found growing in the form of a colony surrounded by a clear zone of inhibition where S. lutea had failed to grow. A smear from this contaminant colony was found to contain a Gram positive, chain-ond spore-forming bacillus. The organism was isolated and its antagonism against S. lutea was reshocked. Preliminary investigation of its activity against other bacteria indicated that this activity was directed against 5. lutea only. I was decided to continue the investigations with two aims in …