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The Fate Of Iron Released From Heme By Hemeoxygenase-1, Jonathan Mark Gardner
The Fate Of Iron Released From Heme By Hemeoxygenase-1, Jonathan Mark Gardner
Undergraduate Honors Capstone Projects
A strain of Escherichia coli was genetically modified to co-express human heme oxygenase-1 and ferritin. The E. coli were then grown with varying amounts of hemin to see if the iron released upon degradation of the hemin by heme oxygenase-1 is loaded into ferritin. Following incubation, the ferritin was purified and the amount of iron loaded into ferritin determined. It was found that ferritin purifed from E. coli expressing human heme oxygenase-1 contained more iron than E. coli that did not contain human heme oxygenase-1. It was concluded that some of the iron released upon degradation of hemin by heme …
Bioavailability/Toxicity Of Iron From Aerobically Processed Organic Fertilizer, Stacey Marie Wilson
Bioavailability/Toxicity Of Iron From Aerobically Processed Organic Fertilizer, Stacey Marie Wilson
Undergraduate Honors Capstone Projects
For an undergraduate Honor's project, I worked under the direction of Dr. Jeffery Hall to determine the bioavailability and toxicity of iron from Milorganite® fertilizer, an aerobically processed organic fertilizer. The Milorganite® Company is a subsidiary of the Milwaukee Municipal Sewage District and produces a fertilizer of high iron content (approximately 5-7%). The high iron content has resulted in concern about the toxic potential, which until now was unknown. This thesis paper will explain iron chemistry, bioavailability, requirements, absorption, toxicity, and treatments, followed by a description of fertilizer types and contents. This will be followed by a description and reasoning …