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Undergraduate Honors Capstone Projects

Analysis

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Analysis Of An Inkjet Printed Strip Assay For Pregnancy Test In Cattle, Alexandra Windley Kelley Jan 2013

Analysis Of An Inkjet Printed Strip Assay For Pregnancy Test In Cattle, Alexandra Windley Kelley

Undergraduate Honors Capstone Projects

Using commercially available antibodies, we created a modified ELISA strip assay to determine pregnancy in cattle. The anti‐progesterone antibody was printed on small membrane strips via an Inkjet printer. The strips were subsequently analyzed using varying concentrations of progesterone. The objective of this study was to assess this novel antibody printing process and to determine the binding activity of the anti‐progesterone antibody. The long‐term objective is to develop a pen-side pregnancy test that would cost less than $1.00. In this specific study, it was determined that the anti‐progesterone antibody used did not provide adequate sensitivity for visualization required for pen‐side …


Utilizing Single Nucleotide Polymorphism Analysis In Determining Parentage Of Cattle, Nicole M. Elbert Jan 2013

Utilizing Single Nucleotide Polymorphism Analysis In Determining Parentage Of Cattle, Nicole M. Elbert

Undergraduate Honors Capstone Projects

Parentage identification within cattle herds is an important aspect of record keeping. It is essential for accurate registration within a purebred association and decision making for production purposes, such as replacement heifer and sire selection. Methods used to identify parentage have evolved from utilizing blood protein antigens, restriction fragment length polymorphism (RFLP) and microsatellites to the current technology of analyzing DNA profiles for differing single nucleotide polymorphisms (SNPs). In this preliminary study we analyzed genotype data from 1,066 samples obtained from various cattle breeds. These breeds included Holstein, Angus, Hereford, Irish Black, Jersey, Red Angus, Limousine, Charolais, Brown Swiss and …


Does Competition Drive Community Structure? An Analysis Of Grasshopper Competition, H. Joseph Lachowski May 1994

Does Competition Drive Community Structure? An Analysis Of Grasshopper Competition, H. Joseph Lachowski

Undergraduate Honors Capstone Projects

lnterspecific competition has been broadly defined as a negative-negative relationship between species that share a limiting resource. The impact of competition on ecological communities is a widely debated topic (Schoener 1982). Thus community structure is very complex and, in addition to competition, can be influenced by many factors, including climate, disease, parasites, and predation (Hairston et al. 1960; Stower & Greathead 1969; Wiens 1977). The importance of competition depends upon the importance of other processes (Welden & Slauson 1986).