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Mattiastrum Pulumurense (Cynoglossinae, Boraginaceae), A New Endemic Species From Türkiye, Asli Doğru Koca, Mustafa Gökmen, Gülderen Yilmaz, Mehmet Yavuz Paksoy

Turkish Journal of Botany

A new species, Mattiastrum pulumurense, has been described from Tunceli in eastern Turkey. It is generally distinguished from other species (except M. racemosum var. scabridum) by a sparsely hairy or glabrescent above-ground part. It is most similar morphologically to M. longipes, M. calycinum and Paracaryum hedgei (P. hedgei is potential a member of the genus Mattiastrum). However, it differs from M. longipes and M. calycinum by its 3-3.5 mm long and linear lanceolate scales, 2.5-4.5 mm long anthers, and 10-15 mm long styles; it differs from Paracaryum hedgei by its 3-3.5 mm long scales, 2.5-4.5 mm long anthers. In addition, …

Novelties In Funalia And The Status Of Fomitella (Polyporaceae, Basidiomycota), Virton Rodrigo Targino De Oliveira, Paulo Roberto De Assis Alves, Nelson Correia De Lima-Júnior, Carla Santos, Nelson Lima, Leif Ryvarden, Josef Vlasák, Tatiana Baptista Gibertoni

Turkish Journal of Botany

During studies on the taxonomical delimitation of some species of Funalia, some inconsistencies were observed in the genus. Based on morphological and phylogenetic analyses, Fomitella supina was positioned within the Funalia clade, thus the synonymization of the genus and the new combination of F. supina are proposed. Funalia glabrorigens is also combinated in the genus, while Coriolopsis hainanensis is suggested as a synonym of F. floccosa and F. rigida is assumed as a distinct species from F. floccosa. A table with morphological comparison of the 10 Funalia species confirmed by molecular analyses is also presented.

Corrigendum To “Two New Species Of Inocybe (Inocybaceae: Agaricomycetes) From Türkiye Based On Morphological Characteristics And Phylogenetic Evidence [Turkish Journal Of Botany 48 (3) 2024 145-154 ], Oğuzhan Kaygusuz, Ditte Bandini, Henning Knudsen, Meike Piepenbring

Turkish Journal of Botany

This corrigendum is to address an issue regarding the manuscript's previous publication, specifically Fig. 5.Regrettably, the authors have noticed that the Figure 5 is missing among the figures although there is an incite reference within the text. To rectify this oversight and ensure the accuracy of the published work, the Figure 5 is included for your reference.

Link to the original article: https://journals.tubitak.gov.tr/botany/vol48/iss3/3/

The Sect. Tricheroides In The Genus Knautia (Caprifoliaceae) Is More Complex Then Known: An Unusual New Species From Türkiye, Candan Aykurt, Mertcan Gülben, Sinem Günaydin, Duygu Sari, Ramazan Süleyman Göktürk, Emirhan Berberoğlu, Serkan Gülsoy

Turkish Journal of Botany

During a biodiversity survey in the Western Taurus Mountains (Antalya, Türkiye), a new species of the genus Knautia (Dipsacoideae, Caprifoliaceae), K. sivridaghense, was discovered. The new species, which is characterized by its dwarf caespitose perennial habit, is found in rocky places under mixed forests at elevations of 1400–1450 m. Knautia sivridaghense is distinguished from its morphologically similar species, K. goecmenii, especially by its dwarf caespitose perennial habit, unbranched stems, length of involucral bracts, and other floral characteristics. Our phylogenetic analyses based on the nuclear ITS and chloroplast petN(ycf6)-psbM regions confirm its placement within Sect. Tricheroides, contributing to the understanding of …

Two New Species Of Inocybe (Inocybaceae: Agaricomycetes) From Turkey Based On Morphological Characteristics And Phylogenetic Evidence, Oğuzhan Kaygusuz, Ditte Bandini, Henning Knudsen, Meike Piepenbring

Turkish Journal of Botany

During a survey of agarics in the Mediterranean region of southwestern Turkey, some noteworthy mushrooms were collected. Among the specimens, two represent new species of Inocybe which are proposed and illustrated in this study as Inocybe pseudogeophylla and I. succinea. Molecular phylogenetic analyses based on a nuc rDNA ITS1-5.8S-ITS2 (ITS) and partial 28S rDNA (LSU) dataset reveal that these species belong to the Inocybe geophylla group. The sequences of the new species form two independent lineages and differ from the known species of Inocybe by a unique combination of morphological characteristics. Detailed macro- and micromorphological features, colour photographs, habitat and …

Cephalaria Gokturkii (Caprifoliaceae), A New Species From Southwestern Anatolia, Türkiye, Gürkan Semi̇z, Meryem Bozkurt, Batıkan Günal, Tuna Uysal

Turkish Journal of Botany

A new Cephalaria (Caprifoliaceae) species, Cephalaria gokturkii, is described and illustrated from Girdev Mountain (Muğla Province) for the Flora of Türkiye. Throughout this manuscript, the new species and its relatives were compared in terms of morphological, palynological and molecular features. The obtained findings indicated that the new species is similar to C. elmaliensis but is characterized by having larger capitula and receptacular bracts in flower (vs. 1‒2 cm capitula and less than 3 mm in flower) and hairy trigonous-narrowly ovate involucral bracts (vs. glabrous and broadly ovate involucral bracts) and smaller involucels (vs. involucels longer than 10 mm in fruit). …

Eco-Assessment Of Phytoplankton Composition In Relation To Environmental Conditions Of Saltwater And Freshwater Lakes In The Konya Closed Basin (Türkiye), Abuzer Çelekli, Ömer Lekesiz, Tolga Çetin

Turkish Journal of Botany

Great importance is given to maintaining lentic ecosystems, threatened by human-induced consequences worldwide. The present study hypothesized to assess differences in species-environment relationships and ecological status of 15 lakes (saline water systems, high-altitude freshwater lakes, and other freshwater lakes) in the Konya Closed River Basin using phytoplankton indices throughout wet and dry periods. Results of canonical correspondence analysis separated saline and freshwater (high-altitude) habitats, which were characterized by different phytoplankton species. Total phosphorus (TP), electrical conductivity (EC), nickel-Ni, and altitude were the most influential environmental factors affecting phytoplankton dispersal in these ecosystems. Saline lakes were under pressure of high EC, …

Identification Of Carrying Alien Dna Fragments In Solanum Melongena X Solanum Incanum Interspecific Progeny By Using Cosii Marker, Görkem Sülü, İlknur Polat, Hati̇ce Fi̇li̇z Boyaci, Ahmet Naci̇ Onus

Turkish Journal of Botany

Cultivated eggplant (Solanum melongena L.) is produced in many countries with temperate and tropical climates and has great economic importance. In recent years, resistance to biotic and abiotic stress conditions in addition to increasing yield and quality has gained importance in plant breeding including eggplant breeding. Therefore, the wild relative S. incanum is an important parent in breeding studies as it provides resistance to some important biotic and abiotic stresses for eggplant. It is possible to obtain a fully fertile hybrid between the two species, as well as to establish F2 lines. However, it is a mystery whether there is …

A New Permineralized Corypha-Type Coryphoid Palm Stem From K-Pg Of India: Anatomy, Systematics, Saprophytic Fungi, And Paleoecology, Ashif Ali, Kaustav Roy, Biswajit Mukherjee, Subir Bera, Mahasin Khan

Turkish Journal of Botany

Palms are known to be an important and diverse angiosperm component in the Deccan Intertrappean beds of Central India. However, the report of fossilized palm stems inhibiting saprophytic fungal remains is empty in the field of paleobotany. Here, we document for the first time the occurrence of fungal remains in a petrified palm stem collected from the latest Maastrichtian (Late Cretaceous)-earliest Danian (early Paleocene) sediments of the Deccan Intertrappean beds of Madhya Pradesh, Central India. The anatomical features of the fossil stem are characterized by the presence of Corypha-type general stem pattern (progressive decrease of fibrous parts of fibrovascular bundles, …

A New Fossil Wood Species Of Ziziphus From The Middle Miocene Of Türkiye And Its Palaeoenvironmental Evaluation, Ünal Akkemi̇k, Özlem Toprak

Turkish Journal of Botany

The purpose of this study is to describe a new fossil wood from the Middle Miocene of South Anatolia (Mersin) and to identify it and evaluate its palaeoenvironmental implications. Thin sections of transverse, tangential, and radial surfaces were examined and described using the terminology of the International Association of Wood Anatomists. The wood was determined to have characteristics of Ziziphus (Rhamnaceae). It is the first fossil wood of Ziziphus and is described as Ziziphoxylon sayaz Akkemik sp. nov. It is from the Middle Miocene Climate Optimum (MMCO), which is a relatively warm interval in the Miocene. Indistinct annual growth ring …

Dorycnium Vuralii (Fabaceae), A New Species From Türkiye, Bilal Şahi̇n, Seher Karaman, Mevlüde Alev Ateş, Zeki Aytaç

Turkish Journal of Botany

The present study describes a new species Dorycnium vuralii (Fabaceae) from Çankırı, Türkiye. The new species is compared morphologically to the taxa of sect. Bonjeanea and sect. Dorycnium along with pollen micromorphology, leaflet, and seed micromorphology. The geographical distribution of Dorycnium vuralii is mapped. The phylogenetic relationships between the new species and other closely related species in the genus are inferred based on DNA data from both cpDNA and nrDNA (rbcL1&ITS). Although phylogenetic implications are different in the two DNA barcoding regions, D. vuralii is shown to be a new species in both molecular data. The new species is assessed …

Isolation And Assessment Of Halophilic Rhizobacteria Plant Growth-Promoting Traits For Alleviating Salt Stress In Wheat, Alev Sezen, Ömer Faruk Algur, Ferruh Aşçi, Arzu Ünal

Turkish Journal of Botany

In this study, 22 halophilic bacteria were isolated from plants collected together with rhizosphere soil from habitats with high salt content in and around Erzurum. Various plant growth-promoting (PGP) properties of these isolates (nitrogen fixing, phosphate solubilizing, ACC deaminase, and IAA and siderephore production) were determined. Bacteria positive for PGP properties and various combinations with these bacteria were subjected to pot experiments in saline medium (greenhouse conditions) and their effects on growth parameters (root and stem length, fresh and dry weight, protein, and chlorophyll and carotenoid content) of wheat plants were determined. As a result of the research, the isolates …

A Comprehensive Study On The Molecular Characterization Of Tomato Spotted Wilt Orthotospovirus Isolates And Resistance Genes In Pepper And Tomato, Qurat Ul Ain Sajid, Emi̇nur Elçi̇

Turkish Journal of Botany

Tomato spotted wilt orthotospovirus (TSWV, Orthotospovirus tomatomaculae) resistance genes (Tsw, Sw5) have been identified in pepper and tomato plants. The development of resistant cultivars is one of the popular management strategies for overcoming viral infections. However, the breakdown of resistance genes has been documented from many places where resistant cultivars have been developed. This has mainly been due to the emergence of resistance-breaking (RB) lines because of the consecutive use of these cultivars. The development of these isolates may cause great losses in both tomato and pepper plants in Türkiye. To investigate the incidence of TSWV, 150 symptomatic samples were …

A Phylogenetic Analysis And Delimitation Of Turanecio (Asteraceae: Senecioneae), Meryem Bozkurt, Tuna Uysal, Ergi̇n Hamzaoğlu

Turkish Journal of Botany

Recently, Turanecio Hamzaoğlu was described as a new genus for the scientific world. Turanecio has been distributed as far as Asia, Iran, Caucasus, and Iraq. A phylogenetic analysis based on nrDNA and cpDNA gene regions was performed to determine the position of the genus within the subtribe Adenostylinae. Results from Bayesian and parsimony analyses of the nuclear and chloroplast DNA regions of the subtribe Senecioninae concordantly indicated that it is monophyletic and belongs to the Quadridentate group, with 4-lobed disc flowers. Nuclear DNA data revealed that some of the researched taxa should be transferred to Turanecio and some taxonomical classifications …

A New Species From Türkiye, Centaurea Turhanii (Cardueae, Asteraceae), Tuna Uysal, Meryem Bozkurt, Ergi̇n Hamzaoğlu

Turkish Journal of Botany

In this article, a new cornflower, Centaurea turhanii Uysal & Hamzaoğlu (Asteraceae) from Türkiye was described and reported. Morphological, karyological, and molecular analyses were used to reveal the taxonomic status of the new species with the species thought to be related to taxa. A modified determination key was submitted and its ecology and phenology were given.

Genetic Variation In White Poplar (Populus Alba L.) Populations As Characterized By Ssr Markers, Burcu Uzan Eken, Emrah Kirdök, Ercan Veli̇oğlu, Yelda Özden Çi̇ftçi̇

Turkish Journal of Botany

The white poplar (Populus alba L.), which is tolerant to abiotic and biotic stresses, is a tree species that is resilient against changing climatic and environmental conditions, which makes it a suitable candidate for afforestation efforts. However, due to prolonged human intervention associated with the increasing population, the genetic resources of this species are at risk of loss. Additionally, while this species generally reproduces sexually, reproduction clonally via its root suckers has become widespread in some areas. Hence, the aim of this study was to investigate the genetic diversity of white poplar, which is increasingly experiencing genetic diversity loss. Herein, …

On The Border Of Extinction And Speciation, Polygala Seyfegoluensis (Polygalaceae), A New Species From Marshy Habitat, Central Türkiye: Under The Threat Of Climate Change, Water Retreat, And Subsequent Degradation, Ali̇ Aslan Dönmez, Zübeyde Uğurlu Aydin, İbrahi̇m Sirri Yüzbaşioğlu

Turkish Journal of Botany

Polygala seyfegoluensis Dönmez, Uğurlu & Yüzb. (Polygalaceae) is herein described and illustrated as a new species from Kırşehir Province around Seyfe Lake in Central Türkiye. This new species is similar to P. pruinosa and P. supina is distinctly characterized by its prostrate habit, nearly glabrous and smaller flower parts, and an unwinged capsule. A phylogenetic analysis was conducted to show the placement of the new species within selected Polygala taxa using the trnL-F and rbcL markers. Based on the field observation, an evolutionary scenario is proposed for the new species under the driving forces of climate change, the shrinking of …

Biochar Influences On Phytochemical Composition And Expression Genes Of Curly Kale At Different Treatment Times, Joyce Dedei Anteh, Esraa Almugrabi, Antonina Mostyakova, Olga Timofeeva

Turkish Journal of Botany

his study examined the effect of biochar application to the soil at different times on the phytochemical composition (phenolic compounds, carotenoids, vitamin C, sugars, proteins, MDA, and antioxidant activity) and expression of HCT, F3’H and CHS genes (which are involved in the accumulation of phenolic compounds) in cabbage kale (Brassica oleracea L. var. acephala). Biochar was prepared from chicken manure, using the method of rapid pyrolysis at a temperature of 400 °C. The retention time at the maximum temperature was 4 h, and the heating rate was 10 °C min–1. The biochar fertilizer was applied to 5–7 day-old kale seedlings …

A Comprehensive Ethnobotanical Survey Of Medicinal Plants For 80 Villages In Trabzon (Türkiye), Sila Ozlem Sener, Kami̇l Çoşkunçelebi̇, Sali̇h Terzi̇oğlu, Aleyna Nalçaoğlu, Tuğçe Peli̇n Gençkaya, Ufuk Özgen, Merve Yüzbaşioğlu Baran

Turkish Journal of Botany

The current study targeted to perform qualitative and quantitative analysis of medicinal plant taxa used in folk medicine in Trabzon. Due to its historical significance, diversity of plant species, and hosting a wide range of cultures, the Turkish city of Trabzon has significant ethnobotanical potential. Ethnobotanical data including family name, scientific name, local name, used parts, therapeutic uses, preparation methods, administration routes, and cited locations were gathered by face-to-face interviews with 645 participants mostly over 50 years old. The quantitative analyses were executed by the determination of frequency values of citation (FC), use value (UV), fidelity level (FL), and informant …

The Impact Of Environmental Factors And Management On The Fitness Of Carlina Acaulis Subsp. Caulescens (Lam.) Schübl. Et G. Martens In Mountain Mesic Meadows, Daniel Pruchniewicz, Ȃngela Lomba, Ludwik Żołnierz, Agnieszka Dradrach, João Pradinho Honrado

Turkish Journal of Botany

The stemless carline thistle (Carlina acaulis subsp. caulescens) is a plant species commonly found at lower altitudes in the Central European mountains. Although it is still relatively frequent in the area, there has been a decrease in its numbers in the last decades. This research aims to determine the impact of the management of mountain mesic meadows and habitat factors on the morphometric variability of C. acaulis and species composition patterns of accompanying vegetation. The study was carried out in the central part of the Sudetes Mountains (SW Poland) on a network of 42 plots randomly established within mesic grasslands. …

A New Species Of Parasola Section Conopileae (Psathyrellaceae) From Pakistan, Zaman Khan, Muhammad Awais Arshad, Abdul Rehman Niazi, Abdul Nasir Khalid

Turkish Journal of Botany

The genus Parasola is sparsely documented in Pakistan with almost all of its reports from lowlands or northern areas of the country. In this study, we describe a new species, Parasola cinnamomescens, from the Thal Desert, a subtropical region located in Punjab Province, Pakistan. Evidence from morphology and DNA sequence data encompassing the internal transcribed spacer (ITS1-5.8S-ITS2 = ITS) and D1/D2 domain of the large subunit of nuclear ribosomal DNA (28S) reveals that this species is divergent from all previously known species and confirms its placement in Parasola sect. Conopileae. It is recognized by convex to hemispherical or broadly conical …

Ethnobotany Of Medicinal Plants Used In Dermatology In Türkiye: A Review, Serpi̇l Demi̇rci̇ Kayiran, Meryem Parlak, Di̇dem Yilmaz Oral

Turkish Journal of Botany

Medicinal plants are increasingly used to treat skin diseases and as cosmetics. Türkiye has a rich diversity of medicinal flora, and patients have a choice of ethnomedicines to manage various dermatology problems. This comprehensive review aims to summarize the primary information available on the dermatological properties of medicinal plants in Türkiye and provide a baseline for the detection of new plant species having efficacy against skin conditions. In this review, clinical and experimental data were researched using relevant search terms in scientific literature databases like PubMed, Web of Science, Google Scholar, ScienceDirect, Scopus, EBSCO, ProQuest, Cochrane Library, and HighWire Press. …

Influence Of Rootstock Age And Gibberellin On The Survival Rate Of Clove (Syzygium Aromaticum L. Merr. & Perr.) Grafting, Ireng Darwati, Rudi Suryadi, Nurliani Bermawie, Octivia Trisilawati, Devi Rusmin, Sri Wahyuni, Siswanto Siswanto, R Vitri Garvita

Turkish Journal of Botany

Clove (Syzygium aromaticum) is a cross-pollinated crop with significant genetic variation in planting materials derived from seeds. Vegetative propagation through grafting is a valuable strategy to achieve genetically identical characteristics in progeny. This study investigates the influence of rootstock age and hormone (gibberellin) treatments on the grafting union and survival rate of top clove grafting under greenhouse conditions. The experiments followed a randomized block design with three replications, each containing 10 samples, and involving two factors: rootstock age (4, 6, and 8 weeks old) and gibberellin (GA3 ) concentrations (0 and 1 mg L–1). The variables observed include success percentage, …

Pollen Morphology Of Scabiosa L. And Lomelosia Raf. (Caprifoliaceae) Taxa In Türkiye, Ahmet Aksoy, Bayram Atasagun

Turkish Journal of Botany

Pollen morphology of 34 taxa belonging to genera Scabiosa and Lomelosia were investigated using a light microscope (LM) and scanning electron microscopy (SEM). Pollen grains of Scabiosa and Lomelosia taxa are tricolpate and triporate, respectively. Pollen shapes vary from oblate to prolate. The exine surface ornamentation can be divided into five types, namely echinate, echinatemicroechinate, echinate-gemmate, echinate-microechinate/gemmate and microechinate-gemmate. Pollen grain sizes were classified as very large in three species of Lomelosia and large in the rest. Statistical analyses revealed some quantitative morphological features, such as equatorial axes, L (polar view amb), and t (apocolpium) as the primary characters for …

Trichoderma Indica: A New Species In The Longibrachiatum Clade From Western Ghats, India, Ajay Lagashetti, Sanjay Singh, Paras Nath Singh

Turkish Journal of Botany

Trichoderma is a hyperdiverse genus that comprises species showing a wide range of applications. In this study, we have found an isolate of the genus Trichoderma from a soil sample collected from Vetal Hill, Pune, Maharashtra State, India. The isolate was found to be morphologically distinct compared to other isolates of the Longibrachiatum clade showing longer phialides and shorter conidia. Moreover, a phylogenetic analysis based on combined sequence data of the second-largest nuclear RNA polymerase subunit (rpb2), and the translation elongation factor 1-α (tef1-α) confirmed the uniqueness of the present isolate, showing an independent branch supported by strong bootstrap values …

Genome-Wide Identification Of Qtls Associated With Iron Deficiency Tolerance In Soybean Reveals Gata12 As The Integrator Of Iron Signaling In Roots, Amir Maqbool, Ahmet Şahi̇n, Allah Bakhsh, Emre Aksoy

Turkish Journal of Botany

Iron (Fe) is an essential micronutrient required for plant growth and development. However, its deficiency causes substantial yield losses, particularly in alkaline soils. Soybean (Glycine max) serves as an ideal model to study Fe deficiency chlorosis (IDC) due to its inefficient Fe uptake from the soil. Although the quantitative trait loci (QTL) associated with IDC tolerance were determined in soybean, the specific genes within these QTL regions remain unidentified. In this study, it was aimed to identify and analyze the expression levels of genes present in IDC-responsive soybean QTL under Fe deficiency. Through this investigation, 6593 genes were identified within …

Beta-Carbonic Anhydrase Gene Expression Levels Change Depending On The Drought Severity In Both The Leaves And Roots Of Arabidopsis Thaliana, Sertan Çevi̇k, Oğuzhan Kurt, Ayşi̇n Güzel Değer, Ferit Kocaçinar, Rıza Bi̇nzet

Turkish Journal of Botany

In this study, it was aimed to determine the relationship between β carbonic anhydrase (βCA) gene expression levels and drought stress severity. For this purpose, the expression levels of six βCA genes in the roots and leaves of Arabidopsis thaliana were investigated under mild, moderate, and severe drought conditions. In addition, changes in the biomass, leaf water content, photosynthetic efficiency, CA enzyme activity, free proline content, and lipid peroxidation were also determined. The results showed that all of the β CA gene expression levels and CA enzyme activity increased in the leaf under mild drought conditions; however, the expression levels …

Nuclear Dna Content And Ploidy Levels Of Living Apple Germplasm Collection In Türkiye, Emel Kaçal, Yaren İpek Şi̇mşek, Turgay Seymen, Şeri̇f Özongun, Meti̇n Tuna

Turkish Journal of Botany

One of the key uses of flow cytometry is the estimation of nuclear DNA content and ploidy level, which has proven a reliable and effective approach in many studies. The primary goal of this research is to estimate the nuclear DNA content and ploidy level of the apple genetic resource collection maintained in the Fruit Research Institute?s living Malus collection for the first time using flow cytometry (for autochthonous varieties). Fresh apple leaf tissues were used for the flow cytometry analysis. For each genotype, nuclear DNA analysis was performed on three individual plants. Propidium iodide (PI) is used as a …

Two New Species Of Tulostoma (Agaricales, Basidiomycota) From The Neotropics, Alexandro Lima, Gislaine Melanda, Paulo Marinho, T A, María Paz Martín, Iuri Baseia

Turkish Journal of Botany

Tulostoma catimbauense and T. deltaconcavum, both collected from Neotropical region, in the Vale do Catimbau National Park, Brazil, are proposed as new to science. Detailed macro- and micromorphological descriptions, including scanning electron microscopy of the basidiospores, are provided. ITS nrDNA sequence analyses were used to investigate the phylogenetic position of these taxa in the genus Tulostoma. Discussions about related species were carried out.

Evaluation Of Phytochemicals And The Role Of Oxidative Stress Pathways During Fruit Development In Strawberries (Fragaria×Ananassa), Fahimeh Mokhtari Shojaee, Elham Mohajel Kazemi, Houshang Nosrati, Maryam Kolahi, Mehdi Haghi

Turkish Journal of Botany

Strawberries (Fragaria × ananassa) are one of the Rosaceae family, considered economically and nutritionally important berry fruits. Phytochemicals are crucial ingredients that contribute to characteristics such as the fruit?s aroma and flavor. The current study investigated the phytochemical characteristics of strawberry fruit at three developmental stages (green, white, and red). HPLC measurements determined the glucose, fructose, and sucrose concentrations significantly increased in the red fruit, whereas glucose was identified as the major sugar in ripe fruit (2.8 g.g-1FW). Evaluation of the phenylpropanoid pathway implied that the amount of total phenol and flavonoid following phenylalanine ammonia-lyase enzyme activity decreased …