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Full-Text Articles in Life Sciences
Cytoplasmically Localized Trna-Derived Fragments Inhibit Translationin Drosophila S2 Cells, Syed Muhammad Hamid, Bünyami̇n Akgül
Cytoplasmically Localized Trna-Derived Fragments Inhibit Translationin Drosophila S2 Cells, Syed Muhammad Hamid, Bünyami̇n Akgül
Turkish Journal of Biology
Transfer ribonucleic acids (tRNAs) serve not only as amino acid carriers during translation but also as a template for the biogenesis of short fragments that can regulate gene expression. Despite recent progress in the function of tRNA-derived fragments (tRFs), their intracellular localization, protein partners, and role in regulating translation are not well understood. We used synthetic tRFs to investigate their localization and function in Drosophila S2 cells. Under our experimental setting, all synthetic tRFs tested were localized at distinct sites within the cytoplasm in a similar manner in Drosophila S2 cells. Cytoplasmically-localized tRFs were positioned in close proximity to GW182 …
Investigation Of The Genotoxic And Antigenotoxic Properties Of Essential Oils Obtained From Two Origanum Species By Drosophila Wing Smart Assay, Eşref Demi̇r, Bülent Kaya, Ricard Marcos, Serap Kocaoğlu Cenkci̇, Hüseyi̇n Çeti̇n
Investigation Of The Genotoxic And Antigenotoxic Properties Of Essential Oils Obtained From Two Origanum Species By Drosophila Wing Smart Assay, Eşref Demi̇r, Bülent Kaya, Ricard Marcos, Serap Kocaoğlu Cenkci̇, Hüseyi̇n Çeti̇n
Turkish Journal of Biology
In this study, essential oils extracted from 2 species of oregano, Origanum onites L. and Origanum minutiflorum O.Schwarz & P.H.Davis, were assayed for genotoxicity using the somatic mutation and recombination test (SMART) in Drosophila melanogaster. Results indicated that there is no significant genotoxic effect of the 2 oils tested. In addition, the antigenotoxic activities of these oils against potassium dichromate (K_{2}Cr_{2}O_{7}) and cobalt chloride (CoCl_{2}) were tested. Results indicated that essential oil cotreatments were able to abolish the genotoxic effects induced by the 2 metal compounds. It is suggested that the observed effects can be linked to the antioxidant properties …
The Effect Of Diazinon On Egg Fertility And Development In Drosophila Melanogaster, Ayla Karataş, Zafer Bahçeci̇, Ensar Başpinar
The Effect Of Diazinon On Egg Fertility And Development In Drosophila Melanogaster, Ayla Karataş, Zafer Bahçeci̇, Ensar Başpinar
Turkish Journal of Biology
This study investigated the effects of diazinon, an organic phosphoric insecticide, on some developmental features of Drosophila melanogaster. The aim was to determine if any developmental inhibition occurs when diazinon is applied and if so, to determine which developmental stages are affected. Therefore, diazinon was applied to Drosophila melanogaster eggs, larvae, and adults, and its effects were observed. During the experiments 3 concentrations with values lower than LC_{50} were utilized (6 × 10^{-5}, 6 × 10^{-6}, 6 × 10^{-7} ppm). Diazinon solution was applied to Drosophila melanogaster by means of nutrition, adding it to the culture medium. The results show …
Anti-Genotoxic Effect Of Ascorbic Acid On Mutagenic Dose Of Three Alkylating Agents, Bülent Kaya
Anti-Genotoxic Effect Of Ascorbic Acid On Mutagenic Dose Of Three Alkylating Agents, Bülent Kaya
Turkish Journal of Biology
The antimutagenic effect of ascorbic acid (vitamin C) was investigated by using the Drosophila wing spot test. In this assay, 3-day-old transheterozygous larvae for the multiple wing hair (mwh, 3-0.3) and flare (flr, 3-38.8) genes were treated with 3 direct acting mutagens: ethyl methanesulfonate (EMS), methyl methanesulfonate (MMS) and N-nitroso N-ethylurea (ENU). The results obtained from 3 reference mutagens were clearly genotoxic in the Drosophila wing somatic mutation and recombination test. The calculated concentration of ascorbic acid as recommended dietary allowance (RDA) did not induce the frequency of the mutant clones recorded. When co-treatment experiments with ascorbic acid were carried …
Lack Of Minority Advantage In Drosophilamelanogaster Mutants, Şükran Çakir, Aykut Kence
Lack Of Minority Advantage In Drosophilamelanogaster Mutants, Şükran Çakir, Aykut Kence
Turkish Journal of Biology
The objective of this study was to test the generality of frequency-dependent fitness in the mating behavior of Drosophila, called the minority effect and to investigate the effect of artificial olfactory cues and marking the flies by wing cillipng on the frequency-dependent mate choice. In the mating experiments using wild type (oregon) and mutant (white-eyed, sepia, ebony) strains of Drosophila melanogaster, the mating success of mutant males did not increase as their frequency decreased. In addition, the olfactory cue hypothesis was not supported by the results of the experiments with artificial scent. The results of this study did not support …