Life Sciences Commons^™

Improving The Quality Of Foliage Transplants For Supply To The Kerry Foliage Industry, Gwen Lanigan

Theses

The possibility of improving the quality of transplants of ornamental foliage species, Eucalyptus perriniana, E. parvifolia and Pittosporum tenuifolium, for supply to the Kerry Foliage Industry was investigated. At present transplants (seedlings) are propagated from seed under glass and subsequently transplanted into the field. Therefore nursery practices for seed propagation, such as optimum sowing date, pot and medium selection, irrigation and fertilisation regimes, were investigated and optimised. The possibility of eliminating the occurrence of J- roots, a major problem with Eucalyptus species was also investigated. Vegetative propagation methods, such as cutting propagation and in vitro micro-propagation were investigated for comparative …

Applications Of The Atomic Force Microscope Within The Fields Of Haematology And Cytogenetics, Margaret O'Reilly-Crowley

Theses

Atomic force microscopy [AFM] has become a rapidly developing interdisciplinary field of research in recent years. The technique, including instrumentation and specimen preparation methods, is now more sophisticated and its application has increased dramatically. The key advantage of AFM is in providing three-dimensional images of samples imaged. Both size and shape of samples imaged can be obtained allowing their subsequent quantification. Red blood cells [RBCs] and chromosomes were imaged in this work. A brief overview of the current status of biomedical AFM, has been presented in the review.

Measurement of mean cell volume [MCV] of immobilised red blood cells with …

Whole Genome Sequencing Of L.Monocytogenes And L.Innocua : A Basis For Comparative Genomics Of The Genus Listeria, Rachel Purcell

Theses

Listeria monocytogenes is a gram-positive, food-borne human and animal pathogen. L.innocua is a non-pathogenic species the most closely related to L. monocytogenes. Comparative genomics between a pathogenic and a closely related non-pathogenic species is a powerful approach for identifying genes or regions on the chromosome, which might be involved in virulence. Therefore whole genome sequencing of these two organisms was undertaken. The strategy used was based on a random approach combined with end-sequencing of a medium insert library for the finishing phase. Based on the available sequence data for these two species an in silico analysis for the presence of …

Pruning And Nutrition Of Outdoor Cut Foliage Crops, Andy Whelton

Theses

The rapidly expanding cut foliage industry in the South of Ireland aims to satisfy the demands of an exacting market requiring well furnished quality stems for use in the manufacture of foliage and flower bouquets. The studies examined, in detail, aspects of pruning and nutrition of genera currently important to this evolving industry. Recommendations for the optimum time of pruning Eucalyptus pulverulenta and Pittosporum tenujolium are given. Pruning regimes for Eucalyptus parv,folia and E. glaucescens, Pittosporum tenufolium, Ozothamnus rosmarinfolius ‘Silver Jubilee’ and Rhododendron ponticum were examined and guidelines on best practices are outlined. A nutrition experiment to examine the effect …

The Molecular Characterisation Of Selected Epidemic Associated Gram Negative Pathogens In Southern Ireland, Mairéad Daly

Theses

This study was undertaken to evaluate various molecular techniques for the analysis of Gram negative bacterial associated epidemics. The first investigation dealt with bovine mastitis outbreaks, where the causative agent was identified as Pseudomonas aeruginosa. Epidemiological findings, suggested that all herds were infected from teat wipes that were contaminated with this organism. Initial investigations using the polymerase chain reaction (PCR), indicated a possible clonal relationship between all outbreak linked strains, with one exception. This finding was confirmed following pulsed-field gel electrophoresis (PFGE) and ribotype analysis. PCR was again employed to study a rather unusual epidemic, involving Salmonella tel-el-kebir over a …

The Molecular Epidemiology Of Rotavirus In Ireland, Fiona O'Halloran

Theses

Between 1997 and 1998, 3,136 cases of rotavirus diarrhoea were detected in Irish children less than 2 years of age. Hospital inpatients accounted for 80% of these infections, with the remainder being diagnosed in general practice. A large percentage of infections were detected in neonates, suggesting a possible inadequate maternal derived protection from existing indigenous strains. This feature suggested the possible existence of ‘novel’ strains circulating in Ireland. No data describing the epidemiology of rotavirus strains in this country currently exists. Furthermore no assessment of the potential health-economic impact or quantitation of potential disease burden was ever undertaken

Three hundred …