Life Sciences Commons^™

Characterization Of The Function Of Pprgtb2 From Mutant Phenotypes, Briana L. Seibert, Hyun Jin Jung, Mark P Running

The Cardinal Edge

Protein prenylation, a common lipid post-translational modification, is required for growth and development in eukaryotes. One type, , but its biological function is not well known. The moss Physcomitrella patens (P. patens) was used as a model organism due to its simple structure, limited cell types, sequenced genome, and its high gene targeting efficiency. P. patens has one copy of Rab-GGT α subunit (PpRGTA1) and two copies of β subunit (PpRGTB1 and PpRGTB2). It has been found that the knockout of either PpRGTB1 or PpRGTB2 results in no visible phenotype, which indicates that these …

Identification Of The Biological Function Of Rab-Ggt Β-Subunits By Reverse Techniques, Briana L. Seibert, Hyun Jin Jung, Mark P. Running

The Cardinal Edge

Abstract

Protein prenylation is a post-translational process where lipids are added to carboxyl end groups of amino acids, which allows proteins to function properly in the eukaryotic cell. The job of prenylation is to target certain proteins to specific membranes and promote desirable protein-protein interactions. In our study we used reverse genetics techniques to investigate the function of protein prenylation in plant development. To discern the function of protein prenylation, we examined the phenotypic changes caused by specific gene disruptions. In this study the model organism Physcomitrella patens (moss) is utilized due to its simple structure, limited quantities of tissues …