Life Sciences Commons^™

Extraneous Background-Correction Program For Matrix Bound Multiple Point X-Ray Microanalysis, W. C. De Bruijn, M. P. C. Van Miert

Scanning Microscopy

A program is described that allows online determination of extraneous background in multiple point X-ray microanalytical matrices. The program is based upon the calculations of the extraneous background for the film (when present), the standard and the unknown by (100 sec.) point analysis. The program searches for a peak-free part of the spectrum in which the calculated value for the extraneous background is about equal to the value in this region of the spectrum (=b_e). Online the contents of this b_e-region is subtracted from an unmanipulated continuum region in the vicinity of the element present in …

Image Analysis And X-Ray Microanalysis In Cytochemistry, W. C. De Bruijn, H. K. Koerten, M. I. Cleton-Soeteman, C. J. G. Blok-Van Hoek

Scanning Microscopy

When cytochemical reaction products are homogeneously distributed within an organelle, point analyses suffice for the quantitative approach. However, quantitative analysis becomes tedious, when the elements in the reaction product are inhomogeneously distributed. Problems arise when elements from two reaction products have to be related to each other, or to endogenous cytological products (ferritin, haemosiderin, calcium, electron dense markers), either topographically or in concentration. When analyzing inhomogeneous/heteromorphical reaction product-containing organelles special attention has to be paid to measure and relate both volume and concentration. In this paper a relative simple structure (eosinophil granules) is chosen to demonstrate that the acquisition of …

Preservation Of In Vivo Morphology Of Blood Vessels For Morphometric Studies, Robert M. K. W. Lee

Scanning Microscopy

Preservation of in vivo morphology of the blood vessels is important for morphometric studies. With each fixation method (e.g., immersion fixation, perfusion fixation), there are inherent pitfalls which can easily distort vessel wall morphology, thereby creating artifacts. Alteration of the vessel wall cell volume due to tissue preparation is another common source for errors. Selection of proper morphometric protocols for comparative studies is crucial. Lack of attention in these areas is a cause of confusion and contradiction in the literature. In this review, the merits of various commonly used methods for vessel wall preparation and measurements are discussed.

Scanning Electron Microscopy In Oral Mucosal Research: A Review, A. Kullaa-Mikkonen

Scanning Microscopy

This review paper highlights some aspects of the contribution of SEM in the field of oral mucosa research. These include 1) different preparative techniques, 2) structure of the oral mucosa and its role in normal function, 3) advances in oral microbiology, 4) development of the oral mucosal epithelium, 5) pathological diagnosis and 6) morphometry.

There are four main ways to study the oral mucosa with SEM; biopsy (autopsy) samples, smears, replica technique, and cell culture techniques. The structural studies can be divided as studies of the surface structure of the superficial cells of the oral mucosa and studies of the …

Morphometric Changes As A Function Of The Proliferative Status Of Murine Mammary Carcinoma Cells, Linda S. Yasui, Lyle A. Dethlefsen

Scanning Microscopy

Ultrastructural analysis was performed to determine morphological changes in the 67 murine mammary tumor cells grown in four defined metabolic states in vitro, i.e., proliferating cells (P), cells in transition towards quiescence (T), nutrient-deprived quiescent cells (Q_I), and Q_I cells stimulated to reenter the cell cycle (St₄) by refeeding for 4h in situ with complete medium. Also, these documented changes were evaluated as a function of the radiosensitivity of the various cell types. The average number of lipid body and mitochondrial profiles per cell was significantly higher in Q_I and St₄ cells …