Life Sciences Commons^™

Scanning Electron Microscopy Of Early Atherosclerosis In Rabbits Using Aortic Casts, Ralph G. Kratky, Margot R. Roach

Scanning Microscopy

Our research involves measuring the size and location of atherosclerotic lesions on the intimal surface of arteries. To this end we have developed a new method in which scanning electron micrographs of vascular casts with impressions of these lesions on their surface are used. This method is compared with light microscopy and scanning electron microscopy of tissue with lesions. All three methods are found to detect lesions equally well. We also examine the cellular elements in the lesion to determine how the images are formed.

The Surface Morphology Of Normal And Atherosclerotic Coronary Arteries In Male Macaca Fascicularis And The Effect Of Coronary Angiography, Dwight A. Bellinger, Jon C. Lewis, Thomas B. Clarkson

Scanning Microscopy

Selective coronary angiography is one of the procedures used frequently in the diagnosis and management of coronary artery disease. Macaca fascicularis monkeys were used to study the effects of coronary angiography on coronary artery surface morphology. Fourteen M. fascicularis were fed either an atherogenic diet (0.34 mg of cholesterol/kcal and 40 to 43% of the calories as fat) for six to nine months or a control diet. For six of these animals the Judkin method of selective left coronary angiography was done 24 h prior to necropsy. The ascending aorta, right coronary artery, left circumflex (LCX), left anterior descending (LAD) …

Developmental Angiogenesis: Quail Embryonic Vasculature, T. J. Poole, J. D. Coffin

Scanning Microscopy

We have examined the segregation and early morphogenesis of the embryonic vasculature by using a monoclonal antibody for immunofluorescence and by scanning electron microscopy. This antibody labels the presumptive endothelial eel ls (PECs) as they segregate from mesoderm. Similar embryos prepared for SEM revealed finer details of how these segregated cells interact to form the rudiments of the major blood vessels. Here we concentrate on the development of the dorsal aortae and the posterior cardinal veins. The dorsal aortae form from single PECs which segregate from the lateral mesoderm and aggregate into a loose cord ventral to the somites. These …

The Role Of The Cytoskeleton In Endothelial Repair, Avrum I. Gotlieb, Michael K. K. Wong, Patricia Boden, Alan Choo Fone

Scanning Microscopy

The injured endothelium undergoes rapid repair of areas of cell desquamation in order to maintain the structural integrity of the endothelial surface. Endothelial repair involves a series of processes which include endothelial cell spreading, translocation, and proliferation. These processes are well defined events which occur sequentially in time. Spreading and translocation are mediated by the cell cytoskeleton - F-actin microfilaments and microtubules and associated centrosomes. The regulation of these processes is complex and is likely due to soluble factors present at the site of injury which are released from activated endothelial cells, platelets, the subendothelial substratum, activated serum factors, and …

An Electron Microscopic Study Of Microfibrils Of Bone Marrow, Ferrell R. Campbell

Scanning Microscopy

After fixation of bone marrow with glutaraldehyde, tannic acid and saponin, a delicate network of microfibrils (10 nm) was observed in the extracellular space. Masses of microfibrils were most frequently observed between the endothelial cells of the sinusoidal wall and adventitial reticular cells, but were also observed at other sites throughout the marrow stroma. Microfibrils are an important component of the extracellular material of bone marrow and appear to provide an anchoring substrate for the endothelium.

Identification Of Non-Denuding Endothelial Injury By Scanning Electron Microscopy, M. Richardson, A. Parbtani

Scanning Microscopy

Endothelial injury is important in the development of arterial disease. To examine the response to endothelial injury it is necessary to recognise if endothelium has been altered and how this relates to normal function. Of the several methods available to evaluate endothelial integrity, ultrastructural examination has certain advantages. In particular, scanning electron microscopy of the endothelium will permit large areas to be examined, and changes can be related to the overall anatomy of the vessel.

There is good evidence that endothelial injury does not inevitably result in endothelial cell loss and denudation of the subendothelium, but that endothelial cells can …