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Full-Text Articles in Life Sciences
Microvascular Casting Of The Lung: Vascular Lavage, Dean E. Schraufnagel, Aloisia Schmid
Microvascular Casting Of The Lung: Vascular Lavage, Dean E. Schraufnagel, Aloisia Schmid
Scanning Microscopy
Corrosion casting is an important tool for the study of microvascular structure, but unlike tissue preparation for light and electron microscopy, few controlled studies of the methods for preparation of microvascular casts for scanning electron microscopy have been reported. Most vascular casting begins with rinsing cells from the lumen of the blood vessels, but the necessity of this has not been shown. Because of a report of successful casting without rinsing and light microscopic evidence of edema with complete rinsing prior to casting, this preparation procedure was examined. Casts of lung vasculature that were thoroughly rinsed were compared to those …
Developmental Angiogenesis: Quail Embryonic Vasculature, T. J. Poole, J. D. Coffin
Developmental Angiogenesis: Quail Embryonic Vasculature, T. J. Poole, J. D. Coffin
Scanning Microscopy
We have examined the segregation and early morphogenesis of the embryonic vasculature by using a monoclonal antibody for immunofluorescence and by scanning electron microscopy. This antibody labels the presumptive endothelial eel ls (PECs) as they segregate from mesoderm. Similar embryos prepared for SEM revealed finer details of how these segregated cells interact to form the rudiments of the major blood vessels. Here we concentrate on the development of the dorsal aortae and the posterior cardinal veins. The dorsal aortae form from single PECs which segregate from the lateral mesoderm and aggregate into a loose cord ventral to the somites. These …
Microvascular Corrosion Casting Of The Lung. A State-Of-The-Art Review, Dean E. Schraufnagel
Microvascular Corrosion Casting Of The Lung. A State-Of-The-Art Review, Dean E. Schraufnagel
Scanning Microscopy
Corrosion casting and viewing of replicas with the scanning electron microscope is an excellent way to study the microvascular structure of the lung. This method can demonstrate aspects of the three-dimensional relationships, branching patterns, maximum diameters, arterio-venous connections, unusual sized and shaped capillaries, development and growth, neovascular structures and changes in development and disease better than any other means. Comparisons can be made in many experimental conditions and fundamental information obtained to answer physiologic questions. This paper reviews how the lung microvasculature has been studied by corrosion casting and scanning electron microscopy and indicates new areas where investigation might be …
Preservation Of In Vivo Morphology Of Blood Vessels For Morphometric Studies, Robert M. K. W. Lee
Preservation Of In Vivo Morphology Of Blood Vessels For Morphometric Studies, Robert M. K. W. Lee
Scanning Microscopy
Preservation of in vivo morphology of the blood vessels is important for morphometric studies. With each fixation method (e.g., immersion fixation, perfusion fixation), there are inherent pitfalls which can easily distort vessel wall morphology, thereby creating artifacts. Alteration of the vessel wall cell volume due to tissue preparation is another common source for errors. Selection of proper morphometric protocols for comparative studies is crucial. Lack of attention in these areas is a cause of confusion and contradiction in the literature. In this review, the merits of various commonly used methods for vessel wall preparation and measurements are discussed.
Vascular Changes In Popliteal Lymph Nodes Due To Antigen Challenge In Normal And Lethally Irradiated Mice, Douglas A. Steeber, Chris M. Erickson, Kees C. Hodde, Ralph M. Albrecht
Vascular Changes In Popliteal Lymph Nodes Due To Antigen Challenge In Normal And Lethally Irradiated Mice, Douglas A. Steeber, Chris M. Erickson, Kees C. Hodde, Ralph M. Albrecht
Scanning Microscopy
The microvascular system of the murine popliteal lymph node was investigated using scanning electron microscopy of microcorrosion casts. Time-dependent changes in the microvasculature following regional antigen challenge in normal and lymphocyte-depleted mice were studied. Normal lymph node microvasculature exhibited a significant increase in both the vascular bed and post-capillary venules containing high-endothelium in response to antigen challenge. Lymph nodes of lymphocyte-depleted mice showed no microvascular size increase following antigen challenge and a reduction in the amount of high-endothelium was observed.