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Full-Text Articles in Life Sciences
Ion And Water Retention By Permeabilized Cells, Carlton F. Hazlewood, Miklos Kellermayer
Ion And Water Retention By Permeabilized Cells, Carlton F. Hazlewood, Miklos Kellermayer
Scanning Microscopy
Nonionic detergents, Triton X-100 and Brij 58, removed, within 2-5 minutes, lipid membrane of suspended thymus lymphocytes and monolayer H-50 cells grown in culture. Studies of hydration, ionic asymmetry, and ionic and protein release kinetics were conducted on these membraneless cellular preparations. The hydration of nuclei isolated by Triton X-100 procedures appears to be influenced strongly by the monovalent ionic concentration of the buffer bathing the organelles. The putative monovalent ionic concentration of the cellular aqueous phase (i.e., 150 meq/L) caused nuclei to swell and coalesce. Monovalent ionic concentrations of 30 meq/L or less caused minimal changes in volume and …
Ex Vivo Platelet Deposition On Fibronectin-Preadsorbed Surfaces, William E. Collins, Deane F. Mosher, Anil R. Diwan, Kedar D. Murthy, Scott R. Simmons, Ralph M. Albrecht, Stuart L. Cooper
Ex Vivo Platelet Deposition On Fibronectin-Preadsorbed Surfaces, William E. Collins, Deane F. Mosher, Anil R. Diwan, Kedar D. Murthy, Scott R. Simmons, Ralph M. Albrecht, Stuart L. Cooper
Scanning Microscopy
Temporal platelet deposition profiles of canine plasma fibronectin (CPFN) adsorbed to different polymers ex vivo and the in vitro characteristics of CPFN adsorption were studied in an attempt to correlate the two. The maximum platelet deposition (𝚪pltmax) obtained at a protein preadsorption time of 30 min was greater than that obtained using an adsorption time of 120 min for all surfaces studied. At 30 min of preadsorption, 𝚪pltmax was 520, 560 and 1230 platelets/1000 μm2 on Biomer, polyethylene (PE) and oxidized PE (OXPE), respectively. In contrast, the platelet deposition at 120 min. of fibronectin …
Utilization Of Immunogold Labeling To Compare The Adsorption Behavior Of Fibrinogen, Fibronectin And Albumin On Polymers, K. D. Murthy, A. R. Diwan, S. R. Simmons, R. M. Albrecht, S. L. Cooper
Utilization Of Immunogold Labeling To Compare The Adsorption Behavior Of Fibrinogen, Fibronectin And Albumin On Polymers, K. D. Murthy, A. R. Diwan, S. R. Simmons, R. M. Albrecht, S. L. Cooper
Scanning Microscopy
Immunogold labeling followed by scanning electron microscopy (SEM) was used to examine the surface distribution of adsorbed plasma proteins. Adsorption was performed under various conditions on six different polymers; [low density polyethylene (PE), chromic acid-oxidized PE (OXPE), solution grade Biomer® (SB), Teflon-(FEP)®, a laboratory synthesized polyurethane containing some zwitterions (ZW) and a polydimethylsiloxane based polyurethane (ZS) also containing zwitterions]. The proteins used were purified human and canine fibrinogen, fibronectin, and serum albumin. The immunogold staining technique was successful in the labeling of the adsorbed proteins. The adsorbed proteins were distributed differently on the polymers selected. Human and canine fibrinogen were …