Life Sciences Commons^™

The Ultrastructure Of Membranes In Sympathetic Ganglia, Catarina Andersson Forsman, Lars-Gösta Elfvin

Scanning Microscopy

This review presents some recent observations made on membranes in the mammalian sympathetic ganglia after application of freeze-fracturing and histochemistry. After freeze-fracture clear differences have been found between the neuronal and the satellite cell plasmalemma. The satellite cell plasma membrane exhibits specialized intramembrane particles not found in the neuronal membrane. Freeze-fracture cytochemistry reveals a further difference between the neurons and satellite cells i.e., a higher density of 𝛃-hydroxysterols is present in the satellite cell membrane than in the neuronal membrane.

Histochemical methods to localize 5'-nucleotidase, Ca²⁺-ATPase and Na⁺/K⁺-ATPase in the membranes have been utilized. …

The Effect Of Glutathione Depletion By Diamide, Diethyl Maleate Or Buthione Sulfoximine On The Surface Structure Of Mouse L-Cells, Joseph G. Szekely, Anya U. Lobreau

Scanning Microscopy

Radiosensitizers that act by reducing glutathione levels have been studied by many workers as agents to enhance the killing of hypoxic tumour cells. In this paper we describe a scanning electron microscope (SEM) study of the surface morphology of mouse L-cells after exposure to three of these: diazenedicarboxylic acid bis N, N-dimethylamide (diamide), DL-buthionine-S, R-sulfoximine (BSO) and diethyl maleate (DEM). Diamide at 0.1 to 0.6 mMol/L for 10 min produced large blebs on the cell surface as seen in the SEM. Transmission electron microscope (TEM) images show that these are clear, featureless regions of cytoplasm. BSO treatment for 24 hat …