Life Sciences Commons^™

Comparison Study Of Mold Growth Resistance Of Plastic Based Material Flooring (Pbm Flooring) And Ceramic Tile Flooring, Jyothi Rangineni, Jeremy Tzeng

Publications

Clemson University Department of Biological Sciences and Tile Council of North America Product Performance Testing Laboratory evaluated whether ceramic tile and Plastic Based Material (PBM¹) flooring support mold growth when exposed to fungal spores.

Mold grows in moisture-rich environments and requires only minimal sources of nutrition to support growth. It has long been identified to cause damage to buildings and construction materials and its presence in buildings has been connected to many major health concerns with various studies and reviews published on this matter.²

The method used to evaluate mold growth was ASTM G21-96 (2015).³ This …

Discovery Of Genomic Variations By Whole-Genome Resequencing Of The North American Araucana Chicken, Rooksana E. Noorai, Vijay Shankar, Nowlan H. Freese, Christopher M. Gregorski, Susan C. Chapman

Publications

Gallus gallus (chicken) is phenotypically diverse, with over 60 recognized breeds, among the myriad species within the Aves lineage. Domestic chickens have been under artificial selection by humans for thousands of years for agricultural purposes. The North American Araucana (NAA) breed arose as a cross between the Chilean “Collonocas” that laid blue eggs and was rumpless and the “Quetros” that had unusual tufts but with tail. NAAs were introduced from South America in the 1940s and have been kept as show birds by enthusiasts since then due to several distinctive traits: laying eggs with blue eggshells, characteristic ear-tufts, a pea …

Gluten Detection Methods And Their Critical Role In Assuring Safe Diets For Celiac Patients, Claudia E. Osorio, Jaime H. Mejias, Sachin Rustgi

Publications

Celiac disease, wheat sensitivity, and allergy represent three different reactions, which may occur in genetically predisposed individuals on the ingestion of wheat and derived products with various manifestations. Improvements in the disease diagnostics and understanding of disease etiology unveiled that these disorders are widespread around the globe affecting about 7% of the population. The only known treatment so far is a life-long gluten-free diet, which is almost impossible to follow because of the contamination of allegedly “gluten-free” products. Accidental contamination of inherently gluten-free products could take place at any level from field to shelf because of the ubiquity of these …

Mitigating The Risk Of Bird Strikes: The Use Of Virtual Reality During Flight Training, Flavio A. C. Mendonca Ph.D., Julius Keller Ph.D., Chien-Tsung Lu, Chenyu Huang Ph.D.

Publications

There were 193,969 wildlife strikes in the US from 1990 through 2017. Wildlife strikes annually cost the US civil aviation industry, on average, $186 million in monetary losses and 111,284 hours of aircraft downtime (Dolbeer & Begier, 2019). According to the Federal Aviation Administration (FAA), Aeronautical Decision-Making (ADM) is a systematic approach that encourages pilots to identify hazards and manage risks (FAA, 2016). Additionally, it helps aviators to make timely and safe decisions. Even though it is practically impossible to eliminate the risk of bird strikes, crewmembers play a vital role in the accident prevention process (Mendonca, Carney, & Fanjoy, …

The Next Frontier: Making Research More Reproducible, David E. Rosenberg, Yves Fillion, Rebecca L. Teasley, Samuel Sandoval-Solis, Jory S. Hecht, Jakobus E. Van Zyl, George F. Mcmahon, Jeffery S. Horsburgh, Joseph R. Kasprzyk, David G. Tarboton

Publications

Science and engineering rest on the concept of reproducibility. An important question for any study is: are the results reproducible? Can the results be recreated independently by other researchers or professionals? Research results need to be independently reproduced and validated before they are accepted as fact or theory. Across numerous fields like psychology, computer systems, and water resources there are problems to reproduce research results (Aarts et al. 2015; Collberg et al. 2014; Hutton et al. 2016; Stagge et al. 2019; Stodden et al. 2018). This editorial examines the challenges to reproduce research results and suggests community practices to overcome …

Beaver Dam Influences On Streamflow Hydraulic Properties And Thermal Regimes, Milada Majerova, Bethany T. Neilson, Brett B. Roper

Publications

Beaver dams alter channel hydraulics which in turn change the geomorphic templates of streams. Variability in geomorphic units, the building blocks of stream systems, and water temperature, critical to stream ecological function, define habitat heterogeneity and availability. While prior research has shown the impact of beaver dams on stream hydraulics, geomorphic template, or temperature, the connections or feedbacks between these habitat measures are not well understood. This has left questions regarding relationships between temperature variability at different spatial scales to hydraulic properties such as flow depth and velocity that are dependent on the geomorphology. We combine detailed predicted hydraulic properties, …

Modeled Microgravity Induces Neutrophil Extracellular Trap (Net)Osis Formation And Reduced Phagocytosis Of Polymorphonuclear Neutrophils, Amber M. Paul

Publications

Spaceflight can dysregulate immunity, by way of increasing granulocytes numbers with impaired function. Polymorphonuclear neutrophils (PMN) are granulocytes that are first responders to infection or injury, and consist of the largest pool of immune cells in humans. PMNs function during innate immunity, through phagocytosis and promotion of inflammation, via the release of reactive oxygen species (ROS) mediators and granule-containing enzymes, such as myeloperoxidase (MPO) and NADPH oxidase-2 (NOX-2). In addition, neutrophil extracellular trap (NET) formation is another mechanism of PMN surveillance that works independently of engulfment phagocytosis, and is a last resort function that can induce NETosis or PMN-specific cell …

Gravity As A Continuum: Effects Of Altered Gravity On Drosophila Melanogaster Immunity, Amber M. Paul, Joe Olivieri, Sharmila Bhattacharya

Publications

The impact of spaceflight on immune function is undoubtedly a critical focus in the area of space biology and human health research. Heat shock proteins (Hsp) are an evolutionarily conserved family of proteins that are expressed in response to cellular and physiological stressors, experienced during radiation exposure, confinement, circadian rhythm disruption, and altered gravity (hypergravity experienced at launch/landing and microgravity experienced in-flight). In particular, Hsp70 aids in the folding of proteins, facilitates the movement of proteins across the membranes during signal transductions and can stimulate innate immunity. Since Hsp70 is induced during cellular stress, and can act as a stimulator …

Characterization Of Sensory And Shelter Enrichment In The Rodent Research Habitat, Amber M. Paul, Yasaman Shirazi-Fard, America Reyes, Moniece Lowe, Margareth Cheng-Campbell, Sungshin Choi, Eduardo Almeida, April Ronca, Ruth K. Globus

Publications

The ISS provides a platform for conducting Rodent Research (RR) in microgravity and 9 missions have been successfully conducted. The results from these experiments have begun to provide new insights into the effects of spaceflight on mammalian physiological systems. After RR-1-4, the Flight IACUC required inclusion of additional cage enrichment into the Rodent Habitats (RH) to “enhance animal well-being by providing animals with sensory and motor stimulation, through structures and resources that facilitate the expression of species typical behaviors”. A Hut, in the form of a rigid, mesh igloo-like shelter was implemented beginning with RR-5. The potential influence of the …

An Analysis Of Common Forest Management Practices For Carbon Sequestration In South Carolina, Lucas Clay, Marzieh Motallebi, Bo Song

Publications

South Carolina (SC) has a variety of different forest types, and they all have potential to sequester a certain amount of carbon. Private forest landowners control a significant portion of the overall forestland in SC, and their management efforts can maintain or improve forest carbon stocks. Currently, the second largest carbon market in the world is the California Carbon Market, which gives a monetary value to sequestered carbon. One carbon credit is equal to one metric ton of carbon and is currently worth around $15.00. Forest management plans are geared toward increasing carbon sequestration over time. This study aims to …

Influence Of Social Isolation During Prolonged Simulated Weightlessness By Hindlimb Unloading, Candice G.T. Tahimic, Amber M. Paul, Ann-Sofie Schreurs, Samantha M. Torres, Linda Rubinstein, Sonette Steczina

Publications

The hindlimb unloading (HU) model has been used extensively to simulate the cephalad fluid shift and musculoskeletal disuse observed in spaceflight with its application expanding to study immune, cardiovascular and central nervous system responses, among others. Most HU studies are performed with singly housed animals, although social isolation also can substantially impact behavior and physiology, and therefore may confound HU experimental results. Other HU variants that allow for paired housing have been developed although no systematic assessment has been made to understand the effects of social isolation on HU outcomes. Hence, we aimed to determine the contribution of social isolation …

Integrative Neuromuscular Training In Young Athletes, Injury Prevention, And Performance Optimization: A Systematic Review, Borja Sañudo, Juan Sánchez-Hernández, Mario Bernardo-Filho, Ellie Abdi, Redha Taiar, Javier Núñez

Publications

The aim of this systematic review was to evaluate the current evidence by assessing the effectiveness of integrative neuromuscular training programs in injury prevention and sports performance in young athletes. Different data sources were analyzed up to January 2018. Eligible studies contained information on population (young athletes), intervention (neuromuscular training), comparator (control group or another exercise intervention), outcomes (injury prevention or sport performance), and study design (randomized trials or prospective studies). The trials were restricted based on the language (English) and for publication date (after 1 January 2007). Fourteen randomized controlled trials were included: Seven included dynamic stability-related outcomes. Three …

Use Of Low-Cost Ambient Particulate Sensors In Nablus, Palestine With Application To The Assessment Of Regional Dust Storms, Abdelhaleem Khader, Randal S. Martin

Publications

Few air pollutant studies within the Palestinian territories have been reported in the literature. In March–April and May–June of 2018, three low-cost, locally calibrated particulate monitors (AirU’s) were deployed at different elevations and source areas throughout the city of Nablus in Northern West Bank, Palestine. During each of the three-week periods, high but site-to-site similar particulate matter less than 2.5 µm in aerodynamic diameter (PM_2.5) and less than 10 µm (PM₁₀) concentrations were observed. The PM_2.5 concentrations at the three sampling locations and during both sampling periods averaged 38.2 ± 3.6 µg/m³, well …

Non-Enzymatic Roles Of Human Rad51 At Stalled Replication Forks, Jennifer M. Mason, Yuen-Ling Chan, Ralph W. Weichselbaum, Douglas K. Bishop

Publications

The central recombination enzyme RAD51 has been implicated in replication fork processing and restart in response to replication stress. Here, we use a separation-of-function allele of RAD51 that retains DNA binding, but not D-loop activity, to reveal mechanistic aspects of RAD51’s roles in the response to replication stress. Here, we find that cells lacking RAD51’s enzymatic activity protect replication forks from MRE11-dependent degradation, as expected from previous studies. Unexpectedly, we find that RAD51’s strand exchange activity is not required to convert stalled forks to a form that can be degraded by DNA2. Such conversion was shown previously to require replication …

Thermal Cues Drive Plasticity Of Desiccation Resistance In Montane Salamanders With Implications For Climate Change, Eric A. Riddell, Emma Y. Roback, Christina E. Wells, Kelly R. Zamudio, Michael W. Sears

Publications

Organisms rely upon external cues to avoid detrimental conditions during environmental change. Rapid water loss, or desiccation, is a universal threat for terrestrial plants and animals, especially under climate change, but the cues that facilitate plastic responses to avoid desiccation are unclear. We integrate acclimation experiments with gene expression analyses to identify the cues that regulate resistance to water loss at the physiological and regulatory level in a montane salamander (Plethodon metcalfi). Here we show that temperature is an important cue for developing a desiccation-resistant phenotype and might act as a reliable cue for organisms across the globe. …

On-The-Road Testing Of The Effects Of Driver’S Experience, Gender, Speed, And Road Grade On Car Emissions, Abdelhaleem I. Khader, Randy S. Martin

Publications

On-road vehicles have become a dominant source of air pollution and energy consumption in many parts of the world. As a result, estimating the amount of pollution from these vehicles and analyzing the factors affecting their emission is necessary to understand and manage ambient air quality. Traditionally, automobile emissions have been measured with dynamometer tests using representative driving cycles. A review of the related literature shows that there is a lack of real life, on-the-road testing of automobile emissions. Moreover, a few previous studies have directly discussed the impact of driver variability on emissions from the vehicles. This research analyzes …

Mice Exposed To Combined Chronic Low-Dose Irradiation And Modeled Microgravity Develop Long-Term Neurological Sequelae, Amber M. Paul, Eliah G. Overbey, William A. Da Silveira, Candice G.T. Tahimic, Sigrid S. Reinsch, Nathaniel Szewczyk, Seta Stanbouly, Charles Wang, Jonathan M. Galazka, Xiao Wen Mao

Publications

Spaceflight poses many challenges for humans. Ground-based analogs typically focus on single parameters of spaceflight and their associated acute effects. This study assesses the long-term transcriptional effects following single and combination spaceflight analog conditions using the mouse model: simulated microgravity via hindlimb unloading (HLU) and/or low-dose γ-ray irradiation (LDR) for 21 days, followed by 4 months of readaptation. Changes in gene expression and epigenetic modifications in brain samples during readaptation were analyzed by whole transcriptome shotgun sequencing (RNA-seq) and reduced representation bisulfite sequencing (RRBS). The results showed minimal gene expression and cytosine methylation alterations at 4 months readaptation within single …

Expanding Beyond Carnivores To Improve Livestock Protection And Conservation, Shari L. Rodriguez, Christie Sampson

Publications

Promoting human–wildlife coexistence is critical to the long-term conservation of many wild animal species that come into conflict with humans. Loss of livestock to carnivore species (e.g., lions, tigers, wolves) is a well-documented occurrence and the focus of mitigation strategies around the world. One area that has received little research is the impact of noncarnivores on livestock. Both African and Asian elephant species are known to cause livestock injuries and deaths. Livestock owners within elephant ranges perceive elephants as a risk to their livestock, which may reduce their tolerance towards elephants and jeopardize conservation efforts in the area. Though feral …

Evaluation And Enhancement Of Carbon Sequestration Potential Of Existing Vegetation Along Roadsides, Vikram Kapoor, Jeffrey Hutchinson, Samer Dessouky

Publications

The objectives of this study were to evaluate the vegetative composition and carbon sequestration potential of vegetation along a major roadway in Texas. Soil and vegetation were evaluated along IH-35 within Bexar County for composition and carbon content. Three 20 m transects were placed at each site and percent vegetative cover was estimated and above ground plant biomass, and soil was collected from three 0.25 m2 subplots along each transect. Plant and soil samples were analyzed for carbon content. Two non-native grasses, bermudagrass and King Ranch bluestem, were the dominant cover at all sites accounting for > 90% coverage at several …

Bird Hazard Mitigation Training For Part 141 General Aviation Pilots: An Experimental Study, Flavio A. C. Mendonca, Richard O. Fanjoy Phd, Thomas Q. Carney

Publications

From 1990 through 2018 ==> 209,950 wildlife strikes in the U.S.,

Approximately 95% of those incidents involved birds;

Seventy aircraft destroyed as a result of wildlife strikes!

13 bird strikes 32 ==> fatalities;

• 244 wildlife strikes ==> 319 people injured!
• 224 bird strikes ==>299 people injured!

General aviation community;

• 97% of the strikes occurred below 3,500 feet AGL;
• There were 22,775 wildlife strikes ==> 26% caused damage to the aircraft!

Wildlife Hazard Management: An Analysis Of Wildlife Strikes Data From The Five Busiest Airports In Brasil: 2011-2018, Flavio A. C. Mendonca, Julius Keller, Carlos F. G. Schonhardt

Publications

Brasil ==> the largest country in Latin America - 8.514.876 Km²;

Booming aviation industry;

• From 2008 through 2017 ==>7.1% per year;
• 104 million passengers in 2018;
• International destinations – 21.8 million (~20%);
• U.S. - 4.4 million passengers;
• Argentina – 3.8 million passengers;
• Portugal & Chile – 1.7 million passengers;
• Brazilian Airlines – 38.3%.

Domestic Passengers 200 million in 2030; International Passengers 55 million in 2030.

Folian-Cv1 Is A Member Of A Highly Acidic Phosphoprotein Class Derived From The Foliated Layer Of The Eastern Oyster (Crassostrea Virginica) Shell And Identified In Hemocytes And Mantle, Mary Beth Johnston, A. P. Wheeler, Elizabeth P. Falwell, Meg E. Staton, Christopher A. Saski, Andrew S. Mount

Publications

The proteins derived from the foliated shell layer of the oyster, Crassostrea virginica, are unusually acidic and highly phosphorylated. Here we report the identification of a gene encoding a member of this class of phosphoproteins that we collectively refer to as folian. Using an in silico approach, a virtual probe was constructed from an N-terminal sequence (DEADAGD) determined for a 48 kDa folian phosphoprotein and used to screen an oyster EST databank. A sequence that matched the N-terminus of the 48 kDa protein was found and used to identify the full-length gene from a C. virginica BAC library. The …

Reduced Gravity Contributes To Neutrophil To Lymphocyte Ratio Shifting And Promotion Of The Oxidative Stress Response, Amber M. Paul, Siddhita D. Mhatre, Egle Cekanaviciute, Ann-Sofie Schreurs, Candice G.T. Tahimic, Ruth K. Globus, Brian Crucian, Sharmila Bhattacharya

Publications

Spaceflight can cause immune system dysfunction, such as elevated white blood cells (WBC) and polymorphonuclear neutrophils (PMN), along with unchanged or reduced lymphocyte counts. A high PMN to lymphocyte ratio (NLR) can acts as a poor prognosis in cancer and a biomarker for subclinical inflammation however, the NLR has not been identified as a predictor of astronaut health during spaceflight. CBC data collected on board the International Space Station (ISS) was repurposed to determine the granulocyte to lymphocyte ratio (GLR) in humans and the NLR in rodents. The results displayed a progressive increase in GLR and NLR during spaceflight and …

Rising Rural Body-Mass Index Is The Main Driver Of The Global Obesity Epidemic In Adults, Con Burns, Tara Coppinger, Janette Walton, Et Al

Publications

Body-mass index (BMI) has increased steadily in most countries in parallel with a rise in the proportion of the population who live in cities1,2. This has led to a widely reported view that urbanization is one of the most important drivers of the global rise in obesity3,4,5,6. Here we use 2,009 population-based studies, with measurements of height and weight in more than 112 million adults, to report national, regional and global trends in mean BMI segregated by place of residence (a rural or urban area) from 1985 to …

Seed Rain Along A Gradient Of Degradation In Caribbean Dry Forest: Effects Of Dispersal Limitation On The Trajectory Of Forest Recovery, Brett T. Wolfe, Raúl Macchiavelli, Skip Van Bloem

Publications

Questions

Tropical dry forests that experience severe disturbances (e.g., fires) often remain degraded for long time periods, during which non-native grasses and trees dominate. One barrier to native tree regeneration in degraded areas may be seed dispersal limitation. To better understand how dispersal limitation influences recovery from degradation, we tested whether the mode and rates of seed dispersal differed in degraded sites dominated either by the exotic tree Leucaena leucocephala or open areas dominated by introduced pasture grasses. We also tested whether L. leucocephala stands facilitate the recruitment of native trees by increasing their seed input compared to open grass …

Effects Of Altered Gravity On The Central Nervous System Of Drosophila Melanogaster, Amber M. Paul, Siddhita Mhatre, Janani Iyer, Jhony A. Zavaleta, Ravikumar Hosamani

Publications

A comprehensive understanding of the effects of spaceflight and altered gravity on human physiology is necessary for continued human space exploration and long-term space habitation. Spaceflight includes multiple factors such as microgravity, hyper gravity, ionizing radiation, physiological stress, and disrupted circadian rhythms and these have been shown to contribute to pathophysiological responses that target immunity, bone and muscle integrity, cardiovascular and nervous systems. In terrestrial conditions, some of these factors can lead to cancer and neuroimmunological disorders. In this study, we used a well-established spaceflight model organism, Drosophila melanogaster, to assess spaceflight-associated changes in the nervous system. We hypothesize that …

Cape Elizabeth Culvert And Habitat Assessment Study, Steve Harding, Jake Aman, Matthew Craig, Robert Malley, Maureen O'Meara

Publications

Executive Summary:

In early 2017, Jake Aman, representing the Wells National Estuary Research Reserve (WNERR), met with the Cape Elizabeth Public Works Director and Town Planner to talk about culverts. Jake shared aerial photos of culverts located in the Spurwink Marsh where scouring of adjacent habitat areas was evident. He inquired if the town was considering any culvert replacements, in which case WNERR and The Nature Conservancy might be able to partner with the town to promote a habitat sensitive replacement. The outcome of the meeting was a jointly sponsored assessment of major town culverts, including those located in the …

Long Reach Lane At Long Marsh, Harpswell, 2018 Post-Project Monitoring Report; Year 5 Of 5, Matthew Craig

Publications

No abstract provided.

Evaluation Of Soybean [Glycine Max (L.) Merr.] Genotypes For Yield, Water Use Efficiency, And Root Traits, Harrison Gregory Fried, Sruthi Narayanan, Benjamin Fallen

Publications

Drought stress has been identified as the major environmental factor limiting soybean [Glycine max (L.) Merr.] yield worldwide. Current breeding efforts in soybean largely focus on identifying genotypes with high seed yield and drought tolerance. Water use efficiency (WUE) that results in greater yield per unit rainfall is an important parameter in determining crop yields in many production systems, and is often related with crop drought tolerance. Even though roots are major plant organs that perceive and respond to drought stress, their utility in improving soybean yield and WUE under different environmental and management conditions are largely unclear. The …

Biotechnological Application In Aquaculture And Its Sustainability Constraints, Olaganathan Rajee, Tang Kar Mun Alicia

Publications

The valuable nutritional and biochemical properties have made fisheries products one of the most vital high-quality protein sources for human consumption. Aquaculture has become the great alternative to substitute wild catches when the yield from fishing are no longer sufficient to sustain the massive food demand of the human population which is constantly burgeoning. However, aquaculture requires multidisciplinary approaches with holistic and environmental-friendly management measures to ensure its long term success and sustainability. Biotechnological applications have enhanced the effectiveness and cost-efficiencies of aquaculture by augmenting the productivity of aquaculture to meet global needs. Despite the benefits, the biotechnological application in …