Life Sciences Commons^™

Development Of Highly Variable Microsatellite Markers For The Tetraploid Silene Stellata (Caryophyllaceae), Juannan Zhou, Michele R. Dudash, Charles B. Fenster, Elizabeth A. Zimmer

Natural Resource Management Faculty Publications

Premise of the study:We designed and tested microsatellite markers for the North American native species Silene stellata (Caryophyllaceae) to investigate its population genetic structure and identify selection on floral design through male reproductive success.

Methods and Results: A total of 153 candidate microsatellite loci were isolated based on next-generation sequencing. We identified 18 polymorphic microsatellite loci in three populations of S. stellata, with di- or trinucleotide repeats. Genotyping results showed the number of alleles per locus ranged from six to 45 and expected heterozygosity ranged from 0.511 to 0.951. Five of these loci were successfully amplified in S. …

Climate Change Impacts On Freshwater Wetland Hydrology And Vegetation Cover Cycling Along A Regional Aridity Gradient, Philip A. Fay, Glenn R. Guntenspergen, Jennifer H. Olker, W. Carter Johnson

Natural Resource Management Faculty Publications

Global mean temperature may increase up to 6°C by the end of this century and together with precipitation change may steepen regional aridity gradients. The hydrology, productivity, and ecosystem services from freshwater wetlands depend on their future water balance. We simulated the hydrology and vegetation dynamics of wetland complexes in the North American Prairie Pothole Region with the WETLANDSCAPE model. Simulations for 63 precipitation × temperature combinations spanning 6°C warming and −20% to +20% annual precipitation change at 19 locations along a mid-continental aridity gradient showed that aridity explained up to 99% of the variation in wetland stage and hydroperiod …

Consequences Of Hatch Phenology On Stages Of Fish Recruitment, David M. Bogner, Mark A. Kaemingk, Melissa R. Wuellner

Natural Resource Management Faculty Publications

Little is known about how hatch phenology (e.g., the start, peak, and duration of hatching) could influence subsequent recruitment of freshwater fishes into a population. We used two commonly sympatric fish species that exhibit different hatching phenologies to examine recruitment across multiple life stages. Nine yellow perch (Perca flavescens) and bluegill (Lepomis macrochirus) annual cohorts were sampled from 2004 through 2013 across larval, age-0, age-1, and age-2 life stages in a Nebraska (U.S.A.) Sandhill lake. Yellow perch hatched earlier in the season and displayed a more truncated hatch duration compared to bluegill. The timing of hatch influenced recruitment dynamics for …

Rapid Surface Water Volume Estimations In Beaver Ponds, Daniel J. Karran, Cherie J. Westbrook, Joseph M. Wheaton, Carol A. Johnston, Angela Bedard-Haughn

Natural Resource Management Faculty Publications

Beaver ponds are surface water features that are transient through space and time. Such qualities complicate the inclusion of beaver ponds in local and regional water balances, and in hydrological models, as reliable estimates of surface water storage are difficult to acquire without time and labour intensive topographic surveys. A simpler approach to overcome this challenge is needed, given the abundance of the beaver ponds in North America, Eurasia and southern South America. We investigated whether simple morphometric characteristics derived from readily available aerial imagery or quickly measured field attributes of beaver ponds can be used to approximate surface water …

Impacts Of Mowing Treatments On Smooth Bromegrass (Bromus Inermis) Belowground Bud Bank, L. Xu, J. Young, A. Boe, J. R. Hendrickson, N. H. Troelstrup Jr.

Natural Resource Management Faculty Publications

Introduced in the 1880s for improving forage production and controlling soil erosion, smooth bromegrass (Bromus inermis Leyss) has invaded and is threating numerous native prairie ecosystems and wildlife habitats in the Northern Great Plains. Land managers of the mixed-grass prairie ecosystems currently spend significant resources attempting to control invasive species and restore native grasslands with various management strategies including grazing, prescribed burning, herbicide application and seeding native species. Unfortunately, many studies have showed that such management efforts have minimal short-term effects. Without sustained effort, persistence and resurgence of smooth bromegrass is inevitable.

Research Update: Bud Bank Ecology For Understanding Perennial Grass Persistence, Lan Xu

Natural Resource Management Faculty Publications

Grassland ecosystems often demonstrate very remarkable resiliency to severe natural and anthropogenic disturbances. Such resiliency following disturbances comes from either seed banks (germinable seeds in the soil) or bud banks (meristems or buds, such as bulbs, bulbils, and buds on rhizomes, corms, and tubers, that generate vegetative tissues). Although seeds are important for dispersal, initial colonization, and maintenance of genetic diversity; few grass seeds persist in the soil more than five years, plus seed production often is unreliable under grazing. Recent studies have demonstrated that >99% of aboveground stems in undisturbed tallgrass prairie were recruited from the bud bank while <1% were recruited from the seed bank. Even under grazed or disturbed sites in tallgrass prairie, belowground buds make a significantly larger contribution (80%) to plant recruitment than do seeds (20%).

Greater Bud Outgrowth Of Bromus Inermis Than Pascopyrum Smithii Under Multiple Environmental Conditions, Jacqueline P. Ott, Jack L. Butler, Yuping Rong, Lan Xu

Natural Resource Management Faculty Publications

Tiller recruitment of perennial grasses in mixed-grass prairie primarily occurs from belowground buds. Environmental conditions, such as temperature, soil moisture and grazing can affect bud outgrowth of both invasive and native perennial grasses. Differential bud outgrowth responses of native and invasive species to climate change and grazing could alter competitive interactions that have implications for future land management. The aims of this work were to (i) compare how spring temperature altered bud outgrowth of native Pascopyrum smithii (Rydb.) Á. Löve (western wheatgrass) and introduced Bromus inermis Leyss.(smooth brome), (ii) compare how watering frequency altered bud outgrowth of these two species …

Invasive Grasses Consistently Create Similar Plant-Soil Feedback Types In Soils Collected From Geographically Distant Locations, Lora B. Perkins, Gary Hatfield, Erin K. Espeland

Natural Resource Management Faculty Publications

Aims Plants of similar life forms and closely related species have been observed to create similar types of plant–soil feedbacks (PSFs). However, investigations of the consistency of PSFs within species have not yielded clear results. For example, it has been reported that species create different types of PSFs in their native and introduced ranges. The aim of this project is to examine if four species create similar PSF types from soils collected from widely distributed areas within their introduced range. The soil for this project was collected from three areas in western North America. With this design, we aim to …

Assessing Temporal Genetic Variation In A Cougar Population: Influence Of Harvest And Neighboring Populations, Rebeca L. Juarez, Michael K. Schwartz, Kristine L. Pilgrim, Daniel J. Thompson, Stephanie A. Tucker, Joshua B. Smith, Jonathan A. Jenks

Natural Resource Management Faculty Publications

The geography of the Black Hills region of South Dakota and Wyoming may limit connectivity for many species. For species with large energetic demands and large home ranges or species at low densities this can create viability concerns. Carnivores in this region, such as cougars (Puma concolor), have the additive effect of natural and human-induced mortality; this may act to decrease long-term viability. In this study we set out to explore genetic diversity among cougar populations in the Black Hills and surrounding areas. Specifically, our objectives were to first compare genetic variation and effective number of breeders of …

Use Of Ecological Sites In Managing Wildlife And Livestock: An Example With Prairie Dogs, John R. Hendrickson, Patricia S. Johnson, Mark A. Liebig, Kevin K. Sedivec, Gary A. Halvorson

Natural Resource Management Faculty Publications

On the Ground

• The perception of prairie dogs among Native Americans living on the Standing Rock Sioux Reservation is mixed. Some Native Americans focus on the loss of forage productivity, whereas others are interested in the cultural and ecological aspects of prairie dogs.
• The use of ecological sites may provide a mechanism for developing a management framework that would consider both livestock and prairie dogs.
• The three ecological sites we surveyed had large differences in off-colony standing crop, but in 2 of the 3 years we surveyed, there were no differences between standing crop on-colony.
• This suggests that management of …

Effects Of Short-Term Cattle Exclusion On Plant Community Composition: Prairie Dog And Ecological Site Influences, Aaron Field, Kevin Sedivec, John Hendrickson, Patricia Johnson, Benjamin Geaumont, Lan Xu, Roger N. Gates, Ryan Limb

Natural Resource Management Faculty Publications

On the Ground

• Maintaining cattle and prairie dogs on rangelands is important ecologically, economically, and culturally. However, competition between these species, both actual and perceived, has led to conflict.
• We explored the effects of short-term (2-year) cattle exclusion on plant communities both on and off prairie dog towns and among three common ecological sites.
• Plant communities were different between on-town and off-town plots and among ecological sites but were similar between cattle-excluded and nonexcluded plots.
• Plant community composition did not differ between rangeland targeted for moderate forage utilization and that in which cattle had been excluded for 2 years.

Effect Of Grazing Prairie Dog—Colonized Rangeland On Cattle Nutrition And Performance: A Progress Report, Kenneth C. Olson, Christopher Schauer, Chanda Engel, Janna J. Kincheloe, Jameson R. Brennan, Ben L. Hauptman

Natural Resource Management Faculty Publications

On the Ground

• One objective of the ongoing Renewal on Standing Rock Reservation project is to evaluate the response of grazing steers to the level of prairie dog colonization on Northern Mixed Grass Prairie.
• We fenced four pastures to create an increasing gradient of a proportion of the pasture area colonized by prairie dogs. Pastures are stocked with yearling steers during each growing season.
• Comparing steer performance, Global Positioning System (GPS) locations of grazing, diet samples, and ingestive behavior at each proportion of the prairie dog colony per pasture allows prediction of the optimal proportion of colonization, which enables selection …

Seed Source May Determine Field-Specific Germination And Emergence: The Source By Planting Environment Interaction, Erin K. Espeland, Lora B. Perkins, Matthew E. Horning, Richard C. Johnson

Natural Resource Management Faculty Publications

Farm environmental conditions and management practices can result in within-cultivar differences in seed quality and lead to transgenerational plasticity (farm-specific effects on offspring, or TGP) that affect germination and emergence in transplant fields. We used three perennial bunchgrasses, [green needlegrass (Nassella viridula) ‘Lodorm’, slender wheatgrass (Elymus trachycaulus) ‘Pryor’, and bluebunch wheatgrass (Pseudoregneria spicata) prevariety registered germplasm P-7] to determine if seeds exhibited TGP. We also determined if TGP was affected by the interaction between production farms and planting environments (farm × environment interaction, or context-dependent TGP), using four laboratory temperature regimes to test germination response and four field environments to …

Winter Habitat Quality But Not Long- Distance Dispersal Influences Apparent Reproductive Success In A Migratory Bird, Clark S. Rushing, Peter P. Marra, Michele R. Dudash

Natural Resource Management Faculty Publications

Long- distance breeding and natal dispersal play central roles in many ecological and evolutionary processes, including gene flow, population dynamics, range expansion, and individual responses to fluctuating biotic and abiotic conditions. However, the relative contribution of long- distance dispersal to these processes depends on the ability of dispersing individuals to successfully reproduce in their new environment. Unfortunately, due to the difficulties associated with tracking dispersal in the field, relatively little is known about its reproductive consequences. Furthermore, because reproductive success is influenced by a variety of processes, disentangling the influence of each of these processes is critical to understanding the …

A Simple Method To Reduce Interpretation Error Of Ages Estimated From Otoliths, Bradley J. Smith, Daniel J. Dembkowski, Daniel A. James, Melissa R. Wuellner

Natural Resource Management Faculty Publications

We designed and tested a novel otolith viewing apparatus termed the otolith illumination device (OID) to ascertain if its use would result in a reduction of interpretation error as determined by increased precision of age estimates obtained from otoliths of walleye Sander vitreus and smallmouth bass Micropterus dolomieu. Clarity of annuli on otolith sections viewed with the OID was generally greater than clarity of annuli on sections viewed with an alternative method. OID-based age estimates were equally as, and in some instance more precise than ages estimated using the alternative method. Additionally, no systematic differences in coefficients of variation …

Validation Of A Technique For Estimating Alfalfa (Medicago Sativa) Biomass From Canopy Volume, Christopher G. Misar, Lan Xu, Arvid Boe, Roger N. Gates, Patricia S. Johnson

Natural Resource Management Faculty Publications

Determining biomass production of individual alfalfa (Medicago sativa L.) plants in space planted evaluation studies is generally not feasible. Clipping plants is time consuming, expensive, and often not possible if the plants are subjected to grazing. A regression function (Bʹ = 0.72558 + 0.11638 × Vʹ) was developed from spaced plants growing on rangeland in northwestern South Dakota near Buffalo to nondestructively estimate individual plant biomass (B) from canopy volume (V). However, external validation is necessary to effectively apply the model to other environments. In the summer of 2015, new data to validate the model were collected from spaced plants …