Life Sciences Commons^™

The Effects Of A Reservoir On Genetic Isolation In Two Species Of Darters, Kerstin Lindsay Edberg

Masters Theses & Specialist Projects

The addition of dams into a riverine system causes a wide range of changes (i.e., sedimentation, erosion, thermal) to the river as well as to the fish assemblages of that river. Although there have been many studies documenting the changes that occur to the fish assemblages in the impounded river, there have been fewer studies examining the effects of a reservoir on the fish inhabiting the tributaries upstream of the impoundment. One possible impact of a reservoir could be to act as a barrier to fish migration between streams.
To determine if reservoirs restrict migration, the genetic diversity of two …

Effects Of A Topical Analgesic Using Massage On Delayed Onset Muscle Soreness, Carrie E. Varvil

Masters Theses & Specialist Projects

No abstract provided.

Cloning Of "Animal Cryptochrome" Cdna From The Model Organism Chlamydomonas Reinhardtii For Functional Analysis Of Its Protein Product, Shobha Lavanya Silparasetty

Masters Theses & Specialist Projects

reinhardtii, a unicellular green alga, is a model organism to study the circadian clock. Cryptochromes are the blue light photoreceptors that entrain the clock in some organisms. The CPH1 protein of C. reinhardtii resembles the cryptochromes of the plant model Arabidopsis, but whether CPH1 entrains the circadian clock in C. reinhardtii is not yet known. Recent reports have suggested the existence of one more cryptochrome in C. reinhardtii, which resembles the cryptochromes of animals. However, the amino acid sequence of this protein shows even higher sequence similarity with the 6-4 DNA photolyase of Arabidopsis. DNA photolyases …

Computational Prediction Of The Agregated Structure Of Denatured Lysozyme, Pongsathorn Chotikasemsri

Masters Theses & Specialist Projects

Mis-folded proteins and their associated aggregates are a contributing factor in some human diseases. In this study we used the protein lysozyme as a model to define aggregation structures under denaturing conditions. Sasahara et al. (2007), Frare et al. (2009, 2006), and Rubin et al. (2008) observed conditions where heat denatured lysozyme formed fibril structures that were observed to be 8-17 nanometers in diameter under the electron microscope. Even though the crystal structure of lysozyme is known, the denatured form of this protein is still unknown. Therefore, we used Rosetta++ protein folding and blind docking software to create in silico …

The Lyme Disease Spirochete, Borrelia Burgdorferi, In Tick Species Collected From Raccoons (Procyon Lotor) And Opossums (Didelphis Virginiana) Trapped In The Warren And Barren Counties Of South Central Kentucky, Kristina Tackett

Masters Theses & Specialist Projects

The incidence of tick-borne zoonoses such as Ehrlichiosis, Rocky Mountain Spotted Fever, and Lyme disease has steadily increased in the southeastern United States in recent years. According to the Centers for Disease Control and Prevention (CDC), the southeastern states accounted for 1,200 of the 27,000 total cases of Lyme disease reported in the U.S. in 2007. Although Ixodes scapularis is the most commonly recognized vector for the Lyme disease spirochete Borrelia burgdorferi, Dermacentor variabilis (a common vector for Rocky Mountain Spotted Fever) also has been shown to be a viable host for this pathogen. The purpose of the present …

Heirloom And Hybrid Tomato Yield And Quality In Organic And Conventional Production Systems, Diana J. Edlin

Masters Theses & Specialist Projects

Due to the recent changes in the economy of Kentucky tobacco production, some producers are seeking an alternative crop that will provide similar economic gains to tobacco without needing more acreage. Tomatoes are an existing crop in Kentucky that have been declining in acreage over the last five years. There is evidence to suggest that, through niche and local marketing, tomatoes may be able to fill the void left by tobacco. However, there is concern among producers that they will lose yield and/or quality if they switch to one of these niche production systems or cultivars.

A two year study …

Using Conservative And Biological Tracers To Better Understand The Transport Of Agricultural Contaminants From Soil Water Through The Epikarstic Zone, Brian Ham

Masters Theses & Specialist Projects

Agriculture contamination is very common in karst systems due to the vulnerability of these aquifers. Animal waste is often spread across crop land to enrich the soil with nitrates and phosphates. Herbicides and pesticides are also applied to the crops. The transport of these pollutants through the soil and epikarst is a difficult process to monitor due to the complex, heterogeneous behavior of the groundwater as it makes its way down to the aquifer below.

An experimental site at Crumps Cave lended a unique opportunity to monitor the vadose zone at a waterfall in the cave below. A previous dye …

In Planta "Green Engineering" Of Variable Sizes And Exotic Shapes Of Gold Nanoparticles: An Integrative Eco-Friendly Approach, Daniel Lee Starnes

Masters Theses & Specialist Projects

Manipulating matter at the nanoscale creates materials endowed with unique optoelectronic and physicochemical attributes. Among the noble metals, the properties of gold in "nano" can be manipulated by varying, their shapes and sizes. Gold nanoparticles find several applications in electronics, medicine and environmental reclamation. Emphasis has been on the “green synthesis” of nanogold to mitigate the hazardous implications stemmed from conventional nanogold synthesis. However, it is not known if the in planta synthesis of nanogold particles could be “green engineered” as well for generating desirable sizes and exotic shapes. In the present study, we used inductively coupled plasma (ICP) analysis …

Prevalence Of Childhood Obesity: A Study On Bowling Green, Ky Middle School Students, Emily Jensen

Masters Theses & Specialist Projects

No abstract provided.

The Development And Role Of Peripheral Auditory Structures In Otocinclus Affinis, Sri Kiran Kumar Reddy Botta

Masters Theses & Specialist Projects

Loricariidae is a very diverse family of catfishes found primarily in the Amazon River basin. These catfishes have a unique characteristic feature of having fenestrae (holes) in the skull region (compound pterotic bone) adjacent to their bi-lobed swim bladder. Since the swim bladders and the compound pterotic may act as an external ear for hearing in this taxon, I hypothesized that these swim bladders structures have an acoustical functional in the loricariid Otocinclus affinis. In order to understand the development of these structures in O. affinis, I first monitored the ontogeny of the compound pterotic bone by clearing …

Effect Of Different Schedules Of Baby Corn (Zea Mays L.) Harvests On Baby Corn Yield, Grain Yield, And Economic Profit Value, Zheng Wang

Masters Theses & Specialist Projects

Maize (Zea mays L.) ranks third as a food crop after wheat and rice and is characterized not only as a cereal crop but also as a vegetable. Maize used as a vegetable is known as “baby corn”. Baby corn consists of unfertilized young ears harvested 2 or 3 days after silk emergence. The present study was implemented in 2009 at Western Kentucky University Agriculture Research and Education Center (36.93 N, 86.47 E) in Bowling Green, Kentucky. The purpose of the study was to compare the effect of different schemes of harvest on baby corn (BC) yield, grain maize (GM) …

Adding Upstream Sequence And A Downstream Reporter To The Bile Acid Inducible Promoter Of Clostridium Scindens Vpi 12708, Bryan Patrick Mason

Masters Theses & Specialist Projects

Bile acids in the small intestines of animals serve to breakdown fats and fatsoluble vitamins. Most of the bile acids are reabsorbed into the enterohepatic circulation, but approximately five percent of these bile acids pass into the large intestine. These bile acids are swiftly deconjugated by the bacterial population, and then subjected to further intestinal bacterial chemical modifications. The most significant of these modifications are 7α-dehydroxylations which form secondary bile acids (deoxycholate and lithocholate). Much research has illuminated the 7α-dehydroxylation pathway: of particular interest is the bile acid inducible operon, for which Clostridium scindens VPI 12708 serves as the model …

Evaluation Of Annotation Performances Between Automated And Curated Databases Of E.Coli Using The Correlation Coefficient, Reddysalilaja Marpuri

Masters Theses & Specialist Projects

This project compared the performance of the correlation coefficient to show similarities in annotations between a predictive automated bacterial annotation database and the curated EcoCyc database. EcoCyc is a conservative multidimensional annotation system that is exclusively based on experimentally validated findings by over 15,000 publications. The automated annotation system, used in the comparison was BASys. It is often used as a first pass annotation tool that tries to add as many annotations as possible by drawing upon over 30 information sources. Gene ontology served as one basis of comparison between these databases because of the limited common terms in the …

The Effects Of Repeated Anaerobic Bouts On Immune Parameters, James C. Sivley

Masters Theses & Specialist Projects

To date, the exercise-induced immune response in has only been evaluated in subjects performing aerobic exercise. The primary purpose of this study is to determine if repeated bouts of anaerobic exercise will induce a similar immune response in human subjects as exhibited by aerobic exercise. Secondary to immune function, an analysis of performance from trial to trial will be made. Nine males between the ages of 18-25 were selected on a volunteer basis to participate in this study. Subjects performed Wingate tests set in 3 series with 120 seconds between trials within series and 405 seconds between series for an …

Searching For The Binding Partners For The Novel Phkg1 Variant Γ 181, Kishore Polireddy

Masters Theses & Specialist Projects

No abstract provided.

Geometric Build-Up Solutions For Protein Determination Via Distance Geometry, Robert Tucker Davis

Masters Theses & Specialist Projects

Proteins carry out an almost innumerable amount of biological processes that are absolutely necessary to life and as a result proteins and their structures are very often the objects of study in research. As such, this thesis will begin with a description of protein function and structure, followed by brief discussions of the two major experimental structure determination methods. Another problem that often arises in molecular modeling is referred to as the Molecular Distance Geometry Problem (MDGP). This problem seeks to find coordinates for the atoms of a protein or molecule when given only a set of pair-wise distances between …

Ecological Impact Of Epigeal Termitaria On Vertebrates In The Tsavo Region Of Southeast Kenya, Margaret M. Mahan

Masters Theses & Specialist Projects

Termites ecologically engineer their environment by producing termitaria (mounds) used by many other species as dens, lookouts or food sources. The role of termite mounds in biological communities is relatively unknown, despite their ubiquitous nature. I investigated their impact on vertebrates in the Tsavo region of Kenya. Through the characterization of mounds, trapping, direct observation, and collecting microclimate data, I was able to determine the importance of mounds to vertebrates. I found uniform dispersion of mounds, that soil type is correlated with the size of mounds, and that vertebrate activity increases with mound size. I also found no significant differences …

Synthesis And Characterization Of Bis-Phosphine Complexes With Transition Metals, Alicia L. Mcdaniel

Masters Theses & Specialist Projects

Extractants and extraction methodologies play a vital role in many industrial processes, from the concentration of precious metals from ores to the separation of longlived nuclei from radioactive waste as well as the removal of heavy metals from soils and water for remediation. The vast majority of extractants rely on the use of nitrogen, oxygen, sulfur or selenium as Lewis base donor atoms to form coordination complexes with the metal ions of interest. These extractants often make use of the chelate effect and/or the macrocyclic effect in order to form stable complexes. Some of the best known types of chelate …

Role Of Cd4+Cd25+ Regulatory T Lymphocytes In Experimental Toxoplasmosis, Sanjay Varikuti

Masters Theses & Specialist Projects

Toxoplasmosis is an important cause of congenital disease, and it is one of the most common opportunistic infections in patients with acquired immunodeficiency syndrome. The need for a reliable experimental model of this infection is crucial not only for achieving a better understanding of the patho-physiology of the disease, but also for developing better methods for evaluating new therapeutic regimens. The purpose of the present study was to investigate the role of CD4+CD25+ T regulatory lymphocytes in mice infected with Toxoplasma gondii. T regulatory (Treg) cells have been shown to play an important role in our immune system in …

Assessment Of The Endangered Species Podarcis Carbonelli On A Microgeographic Scale: A Molecular, Morphological And Physiological Approach, Maria Clara Figueirinhas Do Amaral

Masters Theses & Specialist Projects

The lizard Podarcis carbonelli is an endangered species endemic to the Iberian Peninsula. One location where this species occurs is at the Berlengas Natural Preserve, an Atlantic archipelago off the coast of Portugal. These island populations are geographically separated from nearby mainland populations. The fundamental question is, are these insular individuals distinct from the mainland populations? Four localities were chose for comparison: two island populations and two nearby coastal populations. We assessed this question using three distinct approaches: molecular, morphological and physiological approach. We sequenced the 12S RNA, the mtDNA Control Region and the 7th intron of the !-fibrinogen gene …

The Study Of Variations In The Properties Of Biodiesel On Addition Of Antioxidants, Hiranmayee Kandala

Masters Theses & Specialist Projects

This research studies variations in biodiesel upon addition of different concentrations of antioxidant and petrodiesel additives. Oxidation onset temperature, oxidation induction time, oxidative stability, thermal stability, crystallization onset temperature and moisture retention properties of the biodiesel, with and without antioxidants have been studied. Antioxidants like BHT, BHA, PrG, Vit E and Vit C were added to the biodiesel during the study. These samples were analyzed using the PDSC, TGA, DSC and TGA-SA instrumentation.

The results of this research show an improvement in the OOT, OIT and Oxidative stability of biodiesel with the addition of antioxidants and by blending the biodiesel …

Assessing Organic Matter Breakdown And Associated Macroinvertebrate Community Structure In Headwater Streams: Effects Of Hydrologic Gradients And Upland Timber Harvesting, Miller Scott Jarrell

Masters Theses & Specialist Projects

I examined the effects of hydrologic gradients and upland timber harvesting with different streamside management zone widths on yellow-poplar (Liriodendron tulipifera) processing and the associated macroinvertebrate community structure in the Cumberland Plateau ecophysic region, U.S.A. Prior to upland timber harvesting, 5.0 ± 0.1 g yellow-poplar leaf packs were constructed, zip-tied to gutter nails, and placed into 7 perennial and 6 temporary stream reaches with similar physiochemical and geomorphic characteristics. From December 2007 to May 2008, 3–5 leaf packs were collected per reach monthly. I found significant differences in the functional feeding group composition. Temporary reaches contained higher shredder, gathering-collector, predator, …

The Effect Of Endothelin-1 On The Expression Of Cdk Inhibitors P21 & P27 In Bovine Corneal Endothelial Cells, Lakshmi Reddy Bollu

Masters Theses & Specialist Projects

Mammalian corneal endothelial cells are considered to be non-proliferative due to the arrest of cells at the G1 phase of the cell cycle. The purpose of this study was to determine whether the down regulation of cyclin dependant kinase inhibitors (p21cip1 and p27kip1) levels by Endothelin-1 (ET-1), would overcome the G1 phase arrest and promote cell cycle progression and proliferation in cultured BCECs (Bovine corneal endothelial cells). BCECs were isolated from bovine corneas and cultured in DMEM supplemented with 10% serum. 5-Bromo 2-deoxy Uridine (BrdU) incorporation was determined in serum starved cultures in 24-well plates as a measure of cell …

A Comparative Ecological Study Of Two Sister Species Of Darters In Kentucky, Etheostoma Kantuckeense And Etheostoma Lawrencei, Bjorn Victor Schmidt

Masters Theses & Specialist Projects

Etheostoma kantuckeense and E. lawrencei are former members of the wide ranging E. spectabile species complex. Etheostoma kantuckeense is endemic to the Barren River Basin in Southern Kentucky and Northern Tennessee, while E. lawrencei occurs in the Green River, Salt River, and Cumberland River Basins of Central and Eastern Kentucky. Isolation of populations within these drainages has allowed for a relatively recent evolutionary divergence, leading to slight differences in morphology. This study was conducted to address if geographical isolation has led to measurable differences in the ecology of these two species. In particular, habitat preference across three spatial scales and …

Effects Of Early Spring Growth Annual Ryegrass Pasture Consumption On Parameters Associated With Laminitis In Horses, Morgan Nicole Akers

Masters Theses & Specialist Projects

Ten adult Quarter Horses (5 mares and 5 geldings) were placed in dry lot for 90 days and allowed free choice access to a diet consisting of average quality orchard grass hay, salt and water. The horses were then allowed free choice access to early-growth annual ryegrass pasture, salt and water for a 28 day period. Random hay and grass samples were analyzed for nutrient content. Blood samples were collected at 6 am, 8 am, 6 pm, and 8 pm on the final day of hay consumption and on the 4th, 9th and 28th days of grass consumption. Samples were …