Life Sciences Commons^™

Deep Ocean Vehicle Applications And Modifications, Nichole "Nikki" T. Arm

Master's Theses

This project had two primary goals: (1) to explore opportunities to further a deep-ocean vehicle’s reach using alternative pressure spheres, and (2) to implement an existing deep-ocean vehicle (lander) in active scientific research.

I gained a greater understanding of the limitations and design choices made for existing pressure spheres using Finite Element Analysis (FEA). My simplified FEA model predicted sphere failure for the existing 30% Fiber Glass 70% Nylon injection molded spheres at an external pressure of 3,954psi or 2,690m ocean-depth (only a 7.38% error compared to the tested minimum failure depth), so I determined it a valid model. I …

Evaluation Of Facilitation On Translocation Outcomes For San Joaquin Antelope Squirrel In The Carrizo Plain, California, Patrick R. Anderson

Master's Theses

In arid and semi-arid environments, burrowing mammals play a key role in increasing landscape heterogeneity through facilitative species interactions. The loss of burrowing mammal populations can consequently lead to negative effects cascading through the ecosystem. It is critical to understand these facilitative interactions for conservation and management. Understanding facilitative interactions may improve wildlife management tools, like translocation, which is often not very successful. To investigate the importance of burrow facilitation on San Joaquin antelope squirrel (Ammospermophilus nelsoni; SJAS) translocation success, I designed a natural experiment with a burrowing facilitator, the giant kangaroo rat (Dipodomys ingens; GKR). I …

Using Noninvasive Calibrated Cuff Plethysmography To Observe The Effects Of Cold-Water Immersion On Arterial Compliance, Rita M. Grigorian

Master's Theses

As the prevalence of cardiovascular diseases continues to exponentially grow in populations across the globe, the necessity of determining underlying factors, effective methods of diagnoses, and universally available preventive measures also grows. Early detection of endothelial dysfunction, a proven precursor of cardiovascular diseases, can be extremely impactful in encouraging preventative measures and early intervention before medical conditions become chronic. In recent years, ice plunging, a form of cryotherapy involving full body immersion in cold water, has gained popularity within circles of fitness and health practitioners, gaining the interest of people of all backgrounds. Certain parallels observed between the human physiological …

Physical Treatments To Modify The Functionality Of Carrot Pomace And The Development Of An Enhanced Beef Patty, Jordan O.A Richards

Master's Theses

Approximately one-third of the global food produced for human consumption is lost or wasted somewhere along the food chain. Carrots are one of the most important and widely produced root vegetables grown worldwide; they are rich in many beneficial bioactive compounds such as dietary fiber and carotenoids. Every year, an estimated 29% of processed carrots are lost as waste. The U.S. is the 3^rd largest producer of carrots worldwide, 85% of which come from California. During carrot juice processing, up to 50% of the raw material remains as carrot pomace. Carrot pomace may contain up to 55% total dietary …

Mountain Lion Resource Selection In The California Central Coast: Modeling Habitat Suitability For A Large Carnivore In A Rapidly Changing Environment, Megan Elizabeth O'Connor

Master's Theses

Land use conversion toward agriculture such as orchards and vineyards can have severe negative impacts on habitat and wildlife, particularly large carnivores, globally through habitat fragmentation and loss. The mountain lion (Puma concolor) population in the California Central Coast is thought to provide “stepping-stone” connectivity between several severely genetically compromised coastal populations throughout the Santa Cruz Mountains and several mountain ranges in Southern California; however, the California Central Coast is one of the fastest-developing regions of California with little protection against future land use conversion. Conserving areas of and corridors between high-quality mountain lion habitat through conservation easements …

Mate Choice And Sexual Conflict In A Livebearing Fish, Julia C. Kasper

Master's Theses

Eavesdropping occurs when a receiver extracts information from an interaction without directly engaging with the signaler. Eavesdropping has been shown to be an effective way of evaluating the quality of potential mates and their abilities in male-male competition, without having to directly interact with them, thereby reducing energy costs and mating harassment. Girardinus metallicus is a livebearing poeciliid fish endemic to Cuba whose mating system is dominated by mating harassment in the form of sneak copulations, persistent displaying, and male-male aggression. G. metallicus has a male specific polymorphism in both melanin coloration and behavior. Males with melanin coloration are known …

Evaluation Of Potential Surrogates For Listeria Monocytogenes In Fresh Citrus-Specific Validation Studies, Kimiko Grace Casuga

Master's Theses

The FSMA Produce Safety Rule (PSR) requires citrus packers to more closely assess, manage, and monitor food safety risks. Although there have been no foodborne illness outbreaks and only one recall in fresh citrus, the risk of pathogens coming in on the fruit and cross contamination during washing still exists. Packhouses have dynamic washing systems and in-plant validations may be the only way to demonstrate compliance with the PSR. In-plant validations use surrogates in place of pathogens, and none have been identified or validated for citrus. The aim of this research was to identify a surrogate for use in fresh …

Effects Of Intertidal Position On The Response To Oxygen And Desiccation Stress In The Common Acorn Barnacle, Balanus Glandula, Megan M. Dotterweich

Master's Theses

Sessile invertebrates in the rocky intertidal experience intermittent periods of air exposure due to tidal flux, presenting risks of temperature extremes, hypoxia, nutrient limitation, and most dangerously, desiccation. Microscale variation in severity and frequency of these risks is widely dependent on vertical position within the intertidal zone. Common acorn barnacles (Balanus glandula) have a wide vertical distribution in the intertidal, creating large differences in microhabitat between the highest and lowest individuals in the population. This study set out to explore whether tidal position dependent differences exist in the response to oxygen and desiccation stress in B. glandula. …

Food For Thought: The Effects Of Feeding On Neurogenesis In The Ball Python, Python Regius, Hannah F. Bow

Master's Theses

Pythons are a well-studied model of postprandial physiological plasticity. Consuming a meal has been shown by past work to evoke a suite of physiological changes in pythons and elicit one of the largest documented increases in post-feeding metabolic rates relative to resting values. However, little is known about how this plasticity manifests in the brains of ball pythons, Python regius. Previous work using the cell-birth marker 5-bromo-12’-deoxyuridine (BrdU) has shown that cell proliferation in the python brain increases six days following meal consumption. This study aimed to confirm these findings and build on them in the long term by …

Predicting Location And Training Effectiveness (Plate), Erik Rolf Bruenner

Master's Theses

Abstract Predicting Location and Training Effectiveness (PLATE)
Erik Bruenner

Physical activity and exercise have been shown to have an enormous impact on many areas of human health and can reduce the risk of many chronic diseases. In order to better understand how exercise may affect the body, current kinesiology studies are designed to track human movements over large intervals of time. Procedures used in these studies provide a way for researchers to quantify an individual’s activity level over time, along with tracking various types of activities that individuals may engage in. Movement data of research subjects is often collected through …

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Hydric Physiology Of Lizards, Savannah Weaver

Master's Theses

Chapter 1: Animals can respond to extreme climate by behaviorally avoiding it, or by physiologically coping with it. We understand behavioral thermoregulation and physiological thermal tolerances, but water balance has largely been neglected. Climate change includes both global warming and changes in precipitation regimes, so improving our understanding of organismal water balance is increasingly urgent. We assessed the hydric physiology of endangered Blunt-nosed Leopard Lizards (Gambelia sila) by measuring cutaneous evaporative water loss (CEWL), plasma osmolality, body mass, and body condition throughout their active season. On average, G. sila had low CEWL that is likely desert-adaptive, and high …

Reducing Scope 3 Emissions By Investing In Regenerative Agriculture In Supply Chains, Stephanie Cain

Master's Theses

The agricultural industry has an opportunity to shift to a more sustainable practice that helps restore vital topsoil, improve water quality, reduce environmental impact, and sequester atmospheric carbon into the vast soil carbon pool. However, to implement these practices at considerable scale, agricultural producers require access to resources and capital they rarely have and can be difficult to acquire. As a company, investing in regenerative agriculture in supply chains can lead to reduced Scope 3 emissions, more resilient supply chains, and better marketability as an investment fund, an employer, and a brand. Insetting regenerative agriculture can protect supply chains against …

Southern Sea Otter (Enhydra Lutris Nereis) Fur Morphology, Thermal Function, And Buoyancy Across Ontogeny, Kate Riordan

Master's Theses

Sea otters (Enhydra lutris) have the densest fur of any animal, and the hairs function to trap a layer of air that is used for insulation. When a sea otter is born, it has a natal pelage (baby fur), and sea otters eventually molt that fur and replace it with a pelt resembling the adult fur. Sea otter fur is composed of 3 types of hairs: underhairs, intermediate hairs, and guard hairs. Sea otters are more susceptible to the negative effects of oiling from oil spills compared to other marine mammal species because they solely rely on fur …

Development Of Wiskott-Aldrich Syndrome Knock Out Protocol For Drug Substance Assay Development, Julia C. Hanna

Master's Theses

Wiskott-Aldrich Syndrome (WAS) is a rare X-linked primary immunodeficiency affecting approximately 1 in 100,000 live XY births in North America and is caused by a mutation to the WAS gene which is expressed across hematopoietic lineages. The WAS protein (WASp) plays a role in regulating actin polymerization. On a cellular level, there are a variety of effects of a lack of WASp or expression of a dysfunctional WASp protein for patients including issues with migration, adhesion, chemotactic response, phagocytosis, activation, and proliferation across different cell types in addition to reduced platelet size and output. This can lead to several systematic …

Psf Sampling In Fluorescence Image Deconvolution, Eric A. Inman

Master's Theses

All microscope imaging is largely affected by inherent resolution limitations because of out-of-focus light and diffraction effects. The traditional approach to restoring the image resolution is to use a deconvolution algorithm to “invert” the effect of convolving the volume with the point spread function. However, these algorithms fall short in several areas such as noise amplification and stopping criterion. In this paper, we try to reconstruct an explicit volumetric representation of the fluorescence density in the sample and fit a neural network to the target z-stack to properly minimize a reconstruction cost function for an optimal result. Additionally, we do …

Lichens Of Ultramafic Rocks: A Multidisciplinary Approach To Understanding The Ecology Of An Understudied Organism In A Well-Studied System, Michael Mulroy

Master's Theses

Lichens are among the most prominent and successful life forms of metal-rich habitats, including ultramafic rocks and soils; however, research on lichens of ultramafic habitats is limited, especially on the North American continent. A review of the published literature on lichens of ultramafic substrates in North America yielded a total of 437 lichen species reported from ultramafic rocks and soils. Lichen assemblages of ultramafic substrates vary in composition and are dominated by acidophytic (low pH preferring) taxa with a minor, but consistent, basiphytic (high pH preferring) component. Species lists from ultramafic habitats in different geographic regions varied widely, suggesting that …