Life Sciences Commons^™

Presence Of Antibiotic Resistant Salmonella Spp. In Backyard Poultry And Their Environment, Nicole Land

Master's Theses

As keeping backyard poultry rises, human contact with zoonotic pathogens will increase. One such pathogen that backyard enthusiasts have exposure risks to is Salmonella spp. which may cause a potential public health threat due to its increasing multidrug resistancy. Salmonella spp. were present in 33 of 50 samples collected from 29 sites with backyard poultry coops in San Luis Obispo County during March to May in 2014. Two different Hardy-CHROME™ Salmonella Selective Media plates were used to culture and isolate positive samples of Salmonella spp.. Each positive isolate was tested for antimicrobial sensitivity to 6 standard antibiotics: Ampicillin, Bacitracin, …

Cold Acclimation Response Of Non-Native Italian Wall Lizard (Podarcis Siculus) Populations From New York And California, Daniel Haro

Master's Theses

Understanding how organisms respond to climatic variability and novel conditions is becoming an increasingly important task for ecologists. For ectotherms in the northern hemisphere, the response to cold is of special interest, considering that poleward range expansion events and increasing variability of temperatures during winter are already being observed as consequences of a warming planet. Though direction of change in physiological variables in response to cold is well studied in ectotherms, the extent to which traits can change and the rate at which they can change is not.

We compared the extent and rate of change in cold tolerance (CT …

Molecular Dynamics Of P21 And Fluorescent Sphingomyelin In Keratinocytes Exposed To Uvb, Tyler Malcolm Fraser

Master's Theses

Non-melanoma skin cancer (NMSC) is the most common malignant tumor, representing more than a third of all malignant tumors combined and the incidence is increasing every year. Ultraviolet (UV) radiation from the sun is the most dominant factor contributing to tumor initiation and progression. The condition is most prevalent in populations with lighter skin and older age. Current pharmaceutical molecular research targets the inhibition of the Epidermal Growth Factor Receptor (EGFR), a receptor which is commonly over-expressed or dysregulated in skin malignancies. This study evaluates the content and location of the damage marker p21 within keratinocytes that were incubated in …

Indirect Food Web Interactions: Sea Otter Predation Linked To Invasion Success In A Marine Fouling Community, Maggie F. Jenkins

Master's Theses

Humans have caused grave ecological and economic damage worldwide through the introduction of invasive species. Understanding the factors that influence community susceptibility to invasion are important for controlling further spread of invasive species. Predators have been found to provide biotic resistance to invasion in both terrestrial and marine systems. However, predators can also have the opposite effect, and facilitate invasion. Therefore, recovery or expansion of native predators could facilitate the spread of invasive species. Needles et al. (2015) demonstrated that the threatened southern sea otter (Enhydra lutris nereis) facilitated the invasion of an exotic bryozoan, Watersipora subatra. …

The Thermal Ecology Of Sceloporus Occidentalis, Luis Patricio Burgos

Master's Theses

With temperatures rising globally, assessing the possible impacts of the changing climate becomes more and more urgent. Ectotherms are excellent indicators of potential climatic ramifications on biodiversity because of their heavy reliance on the environment for their thermoregulation. Studies have historically looked at thermal tolerance values to establish predictive models for population and species extinctions.

In chapter 1, we looked at recent studies that suggest that thermal tolerance may be a plastic trait and test the effects empirically. Most studies are based on captive lizards acclimated to laboratory conditions that do not necessarily reflect natural environments, and if thermal tolerance …

Engineering Yeast To Evaluate Human Proteins Involved In Selective Rna Packaging During Hiv Particle Production, Ryan M. Bitter

Master's Theses

Despite recent advances in antiretroviral therapy, nearly 37 million people continue to live with human immunodeficiency virus (HIV). Basic and applied research on the assembly of HIV could be enhanced by using a genetically tractable organism, such as yeast, rather than mammalian cells. While previous studies showed that expression of the HIV Gag polyprotein in Saccharomyces cerevisiae spheroplasts resulted in the production of virus-like particles (VLPs), many questions regarding the utility of yeast in HIV assembly remain uninvestigated. Here, we report use of S. cerevisiae for both the production of VLPs with selectively packaged RNA and to evaluate the human …

Physiological Response Of The Giant Acorn Barnacle, Balanus Nubilus, To Air Exposure, Emily Jane Resner

Master's Theses

The giant acorn barnacle, Balanus nubilus, is a resident of the subtidal and low intertidal rocky shoreline on the Pacific Coast of North America (Alaska to Baja California). B. nubilusis notable for having the largest muscle fibers in the animal kingdom; fiber diameters that can exceed 3mm in adults! At such extreme sizes these muscle cells may be at risk for insufficient oxygen delivery to mitochondria owing to low SA:V ratios and long intracellular diffusion distances. Oxygen limitation to these muscles may be further exacerbated during low tide air exposure (emersion) or environmental hypoxia events, which are increasing …

Parental Perspective And Feeding Practices Effects On Food Neophobia In Elementary-Age School Children, Farnoosh Ayoughi

Master's Theses

The Food neophobia (FN) behaviors in children are developed during childhood and can be influenced by parental FN and feeding behaviors. The objective of this study was to evaluate the relationship between FN and fruit and vegetable neophobia (FVN) among parents, the parents-reports on child's behavior and child self-reports. The effect of parental feeding practices and demographic variables on children’s FN and FVN were evaluated. Sixty-eight parents paired with their elementary school children (aged 7-12 years) in San Luis Coastal Unified School District participated in this study. Results indicated that parents reported their children more neophobic than children self-reported neophobia; …

Effect Of Evapotranspiration Rate On Almond Yield In California, Dafne Isaac Serrano

Master's Theses

Since 2011, California has been under drought conditions. These conditions have not only affected water availability for farmers, but also production. California’s second most valuable crop, almonds, has been affected by drought conditions. This study used three models (Model 1-3) to describe almond yield variability from year to year and almond yield variability within a year in Kern County, CA. The study evaluated 185 almond farms that were classified in three locations (east side, west side and north west side). The years of the study were 2011 (wet year) and 2013-2015 (drought condition years). Model 1 determined a functional regression …

Comparative Analysis Of Body Composition And Reference Values Of Visceral Adipose Tissues In Various American Collegiate Sports, Eshan M. Dandekar

Master's Theses

Background: Currently, body composition (BC) assessment is usually performed to diagnose disease states and accurately estimate certain types of tissues. In athletes, performing BC assessments helps gauge training and nutrition programs to see if they are adequately meeting the athlete’s needs to improve performance. Annual Dual Energy X-ray Absorptiometry (DXA) scans before the start of an athlete’s season can help identify an athlete’s preparedness or health before training begins.

Objective: To assess the preseason BC of four collegiate sports: Men’s basketball, men’s baseball, women’s volleyball, and women’s soccer, for Fat Mass (FM), bone mineral density BMD), Fat-Free Mass …

An Assessment Of The Greatest Impacts On Distribution Uniformity For Drip And Micro Irrigation, Brooke Ashley Worden

Master's Theses

Using the Cal Poly Irrigation Training and Research Centers (ITRC) drip/micro evaluation program, global, or system, DU_lq is computed by combining the component DU_lq values of: pressure variation, uneven spacing between emitters, unequal drainage and “other” causes. “Other” causes include plugging, wear and manufacturing coefficient. The program also computes what percentage of the non-uniformity is due to each component. Burt (2004) showed that over 95% of the non-uniformity is due to “Other” causes and pressure differences. This thesis looks at what specifically in those components is driving the non-uniformity by analyzing various equipment and field practices and their …

Microfluidic Electrical Impedance Spectroscopy, John J. Foley

Master's Theses

The goal of this study is to design and manufacture a microfluidic device capable of measuring changes in impedance valuesof microfluidic cell cultures. Tocharacterize this, an interdigitated array of electrodes was patterned over glass, where it was then bonded to a series of fluidic networks created in PDMS via soft lithography. The device measured ethanol impedance initially to show that values remain consistent over time. Impedance values of water and 1% wt. saltwater were compared to show that the device is able to detect changes in impedance, with up to a 60% reduction in electrical impedance in saltwater. Cells were …

Effect Of Storage Period And Treatment On The Composition And Digestibility Of Olive Pomace In Cattle, Alexis Cecilia Ulloa

Master's Theses

Utilizing local by-products can serve to significantly reduce the current feed costs for ruminant producers. The objective of this study is (1) to evaluate the effects of chemical and bacterial inoculants in combination with ensiling, on the nutritional value and ensiling compatibility of olive pomace (OP) and (2) to identify the most biologically favorable treatment and ensiling day of OP for feeding to cattle. Freshly centrifuged OP (Olivas de Oro Olive Company, Creston, CA) was treated with one of four treatments: (1) no additives (control; CONT); (2) urea applied at 5% of DM (UREA; Agrium U.S. Inc, Loveland, CO); (3) …

Modifying The Mineral Profile Of Crickets (Acheta Domesticus) Using A Supplemented Diet, Rhianne Morgan Le Maxwell

Master's Theses

Captive insectivores may consume invertebrates as all, or part of their overall diet. The challenge with feeding captive insectivores involves the limited number of invertebrate species that are commercially available, and the lack of key nutrients provided by these insects. Among these insects, a naturally occurring low concentration of calcium and an inverse calcium to phosphorus ratio may put insectivores at the risk of developing hypocalcemia. A strategy to correct this nutrient imbalance involves supplementing the insect diet with high concentrations of targeted nutrients – a term referred to as gut-loading. Current industry guidelines recommend feeding a supplemented diet for …

The Roles Of Tid1, Ndj1, And Spo16 In Distributive Segregation During Saccharomyces Cerevisiae Meiosis, Ethan Atticus Shaw

Master's Theses

Meiosis is a specialized form of cell division in sexually reproducing eukaryotes. Crossovers are physical connections formed between homologous chromosomes during meiosis; these connections help ensure normal segregation of homologous chromosomes at meiosis I. However, the yeast Saccharomyces cerevisiae and other eukaryotes can still segregate homologs properly even in the absence of some crossovers. This is due to a backup mechanism known as distributive segregation, which correctly segregates non-crossover chromosomes at a higher rate than if segregation were completely random. To study distributive segregation, we have generated diploid yeast with one homeologous chromosome pair consisting of a Saccharomyces cerevisiae chromosome …

Quantifying The Environmental Performance Of A Stream Habitat Improvement Project, Cody Morse

Master's Theses

River restoration projects are being installed worldwide to rehabilitate degraded river habitat. Many of these projects focus on stream habitat improvement (SHI), and an estimated 60%of the 37,000 projects listed in the National River Restoration Science Synthesis Program focus on SHI for salmon and trout species. These projects frequently lack a sufficient monitoring program or account for the environmental costs associated with SHI. The present study used life cycle assessment (LCA) techniques and topographic effectiveness monitoring to quantify environmental costs on the basis of geomorphic change. This methodology was a novel approach to assessing the cost-benefit relationship of SHI. To …

Detecting A Probiotic Product Within The Gut Of Broiler Chickens, Anneka Pisula

Master's Theses

As of January 2017, the U.S. poultry industry banned the use of antibiotics and now relies on alternatives such as probiotics to help protect animal health. Although probiotic use is not a new concept in the poultry industry, identifying the best combination of bacterial strains to generate an effective probiotic formula requires further investigation. This study aimed to detect a probiotic product of four bacterial strains (Pedioccoccus acidilactici, Pediococcus pentosaceus, Lactobacillus plantarum, and Bacillus subtilis) in a feeding trial with broiler chickens. Birds given the probiotic were predicted to show an improved growth performance with …

Effects Of Polydatin On In Vitro Bovine Embryo Developmental Competence, Metabolism, And Cryopreservation, Corie Marie Owen

Master's Theses

Bovine in vitro produced embryos suffer from poor developmental competence and altered metabolism which hinders their cryotolerance. Overall, the goals of this thesis were to improve oocyte and embryo culture with the antioxidant polydatin and to optimize slow freezing procedures. This thesis was designed as three experiments, and in each experiment, oocytes were aspirated from abattoir ovaries, matured for 23h, fertilized with semen from 1 of 3 bulls, and cultured in synthetic oviductal (SOF) based medium (SCF1) in 38.5 °C in 5% O₂, 5% CO₂ and 90% N₂. Stage 7 blastocysts were stained with Nile …

Assessment Of Granulovirus, Spinosad, And Mating Disruption For Controlling Cydia Pomonella L. [Lepidoptera: Tortricidae] In Organic Coastal California Apple Orchards, Raven Lukehart

Master's Theses

Codling moth, Cydia pomonella[Lepidoptera: Tortricidae], is a major entomological pest of apples, pears, and walnuts cross the world (Pajac et al. 2016). Female codling moths lay eggs on the apple exocarp and larvae burrow within the fruit causing economic losses to fruit growers.Organic apple orchards in San Luis Obispo, CA currently have three codling moth, Cydia pomonella,control options commercially available including granulovirus (CpVG), spinosad, and mating disruption. In field tests on apple (Malus), we compare percent fruit injury between treatments of granulovirus (2.43 oz/ha Cyd-X® organically approved, Certis USA, Columbia MD), spinosad (4.05 oz/ha Entrust® Naturalyte® …

Evaluation Of Endothelial Cell Responses To Elevated Glucose, Gabriella Sugerman

Master's Theses

Developing a tissue-engineered Blood Vessel Mimic (BVM) to represent diabetic macrovascular disease could expedite design of new vascular devices specifically tailored to diabetic patients. In contribution toward this model, this thesis assessed Human Umbilical Vein Endothelial Cell (HUVEC) responses to high glucose conditions. Interleukin 6 (IL-6) and Cluster of Differentiation 36 (CD36) were selected to signify oxidative stress activity, a hallmark of diabetic macrovascular disease. Next, activity of potential reference genes B2M, HPRT1, and ACTB was assessed. All genes were found to exceed acceptable variability, so the E-ΔC T method of data analysis was selected. Next, cellular responses to high …

Crossing Corridors: Wildlife Use Of Jumpouts And Undercrossings Along A Highway With Wildlife Exclusion Fencing, Alex J. Jensen

Master's Theses

Roads pose two central problems for wildlife: wildlife-vehicle collisions (WVCs) and habitat fragmentation. Wildlife exclusion fencing can reduce WVCs but can exacerbate fragmentation. In Chapter 1, I summarize the relevant studies addressing these two problems, with a focus on large mammals in North America. Chapters 2 and 3 summarize field assessments of technologies to reduce WVCs and maintain connectivity, specifically jumpout ramps and underpasses, along Highway 101 near San Luis Obispo, CA. In a fenced highway, some animals inevitably breach the fence and become trapped, which increases the risk of a wildlife-vehicle collision. Earthen escape ramps, or “jumpouts”, can allow …

The Effect Of Destoning And Enzymatic Pretreatments On The Biofuel Production From Olive Cake, Patrick Tai

Master's Theses

More than 16,000 tons of olive cake was produced in the United States in 2017. Olive cake is a by-product of olive oil extraction, which has limited animal feed potential, and poses an environmental threat when landfilled due to its high organic load and polyphenol content. This residue has potential for biofuel (bioethanol and biomethane) production because it is rich in polysaccharides such as pectin, hemicellulose, and cellulose. Yet, olive cake contains olive stones that can impede its conversion to biofuel. Therefore, two methods of destoning, centrifugation and screening by horizontal screw press, were first compared. Both methods removed an …

Analysis Of Potential Vermicompost Market In California, Jason Alan Thomas

Master's Theses

Vermicompost transforms agricultural waste into a high quality soil amendment, though market acceptance remains in its infancy. This study examines how growers’ willingness to pay for vermicompost is affected by grower’s crop, region, income per acre, knowledge level of vermicompost and compost, previous use of compost, and the willingness to pay for compost. The survey results pulled together 223 responses from California growers. It was discovered growers’ had less knowledge of vermicompost than compost but were willing to pay more for vermicompost. There was statistical difference amongst the responses. It was shown the market value per ton of vermicompost lies …

Zinc Sunscreens Affect Development Of Strongylocentrotus Purpuratus Embryos, Brittany E. Cunningham, Nikki L. Adams

Master's Theses

The growing popularity of physical sunscreens will also lead to an increased release of the ingredients from zinc oxide (ZnO) sunscreens into marine environments. Though zinc (Zn) is a necessary micronutrient in the ocean, greater than natural Zn concentrations are being released into marine environments by use of sunscreens. The extent of the consequences of the addition of Zn to the ocean are not fully understood. We investigated effects of materials released by zinc oxide (ZnO) sunscreens on the development of California purple sea urchin, Strongylocentrotus purpuratus. Embryos developed in various concentrations of Zn, the sources of which included …

Foraging Ecology Of Parrotfishes In The Greater Caribbean: Impacts Of Specialization And Dietary Preferences On Marine Benthic Communities, Madelyn V. Roycroft

Master's Theses

Coral reefs are one of the world’s most diverse yet heavily impacted marine ecosystems. As a result of many direct and indirect stressors, coral reefs have experienced major degradation over the last several decades. Declines in coral reefs in the Caribbean have been particularly acute and generally associated with the loss of key herbivores and an increase in algae. Herbivorous fishes such as parrotfishes can positively impact coral reefs by removing algae that compete with corals for light and space. However, many parrotfishes are also important coral predators. Predation on corals, known as corallivory, can adversely affect coral growth, reproduction …

Effect Of Aquaponic Vs. Hydroponic Nutrient Solution, Led Light Intensity And Photoperiod On Indoor Plant Growth Of Butterhead, Romaine And Kale (L. Sativa, B. Oleracea), Sean M. Foster

Master's Theses

Vertical farming has been proposed as a solution for providing food security for an increasing, urbanized human population. Light-emitting diode (LED) technology has become increasingly affordable and efficient, making it an ideal choice as artificial lighting for indoor farms. Still largely undiscovered parameters are the optimal plant varieties and types of production systems for plant growth, profit, and human nutrition. Aquaponics may be able to provide sustainable animal protein for vertical farms, increasing their ability to provide more substantial nutrition to consumers. This research aimed to better understand vertical farming as a food production system, and to determine if aquaponics …

Effect Of Bioaugmentation Product Biowish® Aqua™ On Nitrogen Removal In Wastewater, Patrick Cassidy Kalvass

Master's Theses

Biological nutrient removal (BNR) from wastewater, and specifically nitrogen removal, is a growing concern to wastewater dischargers such as municipalities. Excess nutrients in effluent can create problems such as eutrophication, toxicity to aquatic life, and dissolved oxygen depletion in receiving waters. BNR systems have been installed in many locations with success, but their operation presents operational and financial demands greater than conventional biological treatment. Nitrogen removal is typically performed in sequential autotrophic nitrification and denitrification, which increases needed energy input, operational complexity, and therefore cost. Simultaneous nitrification-denitrification (SNdN) achieved in a single system has also been successfully implemented, however operational …

Comparative Analysis Of Physiological Measurements And Environmental Metrics On Predicting Heat Stress Related Events, Mckenzie Lee Barlow

Master's Theses

Exposure to high heat and humidity can lead to serious health risks, including heat exhaustion and heat stroke. Wet Bulb Globe Temperature (WBGT) and heat index have historically been used to predict heat stress events, but individualized factors are not included in the measurement. It has been shown that there is a relationship between cardiovascular measurements and heat stress, which could be used to measure heat stress risk on an individual level. Research has been done to find relationships between cardiovascular metrics in a workplace environment, however the study did not include the use of a controlled environment as a …

Bird Abundance At Bird Feeders In Response To Temperature, Wind Speed And Precipitation During The Winter Season, Siddhant Kahal

Master's Theses

The goal of this project is to explore how 23 different bird species respond to 3 climatic attributes. These attributes are lower than average temperatures, wind speed and precipitation level. Information about the bird species and all of the data associated with them is provided by Project FeederWatch (PFW). This is a citizen based survey study that provides key information about bird species abundance through the use of backyard and community feeders. The study volunteers from across the United States and Canada monitor these bird feeders and note important information about the species such as the number of individuals seen. …

Pink And Dude Chefs: Effectiveness Of An After-School Nutrition Knowledge And Culinary Skills Program For Middle School Students To Increase Fruit And Vegetable Consumption, Alyssa S. Vaziri

Master's Theses

The rate of overweight and obesity among adolescents aged 12-19 years has more than tripled since 1980, and disproportionately impacts low-income and marginalized populations. Reduction in adolescent obesity rates may result in decreased health risks, decrease healthcare costs, and increased quality of life. Effective intervention methods for adolescent participants have incorporated nutrition knowledge and culinary skill building into afterschool programs. This study examines whether building knowledge, skills, and confidence through a culinary intervention can improve adolescent participants’ choices of healthful foods through increased fruit and vegetable intake.

Pink and Dude Chefs (PDC) is an afterschool nutrition education and culinary skills …