Life Sciences Commons^™

Biochemical Assay Optimization And Computational Screening Efforts To Identify Potential Luxs Inhibitors, Keeshia Q. Wang

Master's Theses

Quorum sensing (QS) is a process of coordination of bacterial gene expression in response to cell population. System two QS is regulated by the small signaling molecule autoinducer-2 (AI-2) and is implicated in the infectious behaviors of various bacterial species. AI-2 is biosynthesized from S-ribosylhomocysteine (SRH) by the enzyme LuxS and induces interspecies cell-to-cell communication. Inhibition of LuxS would therefore inhibit interspecies QS. Herein, a search for novel molecular species that will competitively bind with SRH in the LuxS binding site is performed in silico. Computational screening results are then validated in vitro using an optimized LuxS inhibition …

The Effects Of 8 Weeks Of Low Dose Supplementation Of Creatine And Sodium Bicarbonate On Exercise Performance, Amanda Jessica Morris

Master's Theses

Short-term (3-7 days), high doses of creatine (20g/d) and/or sodium bicarbonate (0.5g/kg body weight) supplementation increase exercise performance during short term high intensity activities; however, it remains unclear whether long-term, low doses of these supplements have a positive impact on exercise performance. The purpose of this study was to determine the effects of long-term (8 weeks), low dose creatine supplementation on exercise performance, and whether combining creatine and sodium bicarbonate supplementation has an additive effect. Sixty-three healthy, habitually active, adults (28 M, 35 W; 22+2 years; 23+ 3 BMI) were randomly assigned by sex to one of three supplement groups: …

An Insight Into The Microbial Diversity And Expression Of Cysteine Protease Inhibitors (Cystatin) In Rickettsia Parkeri Infected Amblyomma Maculatum, Khemraj Budachetri

Master's Theses

Amblyomma maculatum (Gulf Coast tick) is an emerging tick species of public health significance in United States. It is a competent vector of Rickettsia parkeri, an etiological agent of a human rickettsiosis. In this study, we investigated the spotted fever group of rickettsial diversity in A. maculatum based on rickettsial ompA gene PCR. Our results showed A. maculatum harbors R. parkeri, R. amblyommii, and R. endosymbiont of A. maculatum. While only R. parkeri was detected in female salivary glands which suggest its ability to traffic from midgut to salivary glands via hemocoel. The presence of R. parkeri was further …

Urban Agriculture Stormwater Management In California Cities, Rachel L. Cohen

Master's Theses

Cities within California are beginning to incorporate urban agriculture into their land use designations. Prompted by residents and local organizations, cities are hoping to capture the benefits that urban agriculture provides. Research has shown that urban agriculture renews and beautifies neighborhoods, provides healthy food choices, increases public health, has the potential to help with stormwater runoff, creates jobs, and fosters community. In the last few years, several California cities have made headlines as they have adopted new zoning codes that include urban agriculture.

In reviewing these new zoning codes and exploring the topic of urban agriculture, it became evident that …

Associations Between Alcohol Consumption And Fasting Blood Glucose In Young Adults, Julie Ann Lucca

Master's Theses

Current research shows moderate alcohol consumption is associated with decreased risk of diabetes and excessive consumption or binge drinking can cause insulin resistance and diabetes. In 2010, diabetes was the seventh leading cause of death in the United Statesand was responsible for significant health complications: blindness, kidney failure, and limb amputations, and is a large national economic burden. Fasting blood glucose (FBG) is a tool used to help diagnose diabetes. Abnormally high FBG, ≥100 mg/dl, is indicative of diabetes and pre-diabetes. Few studies have observed diabetic prevalence among young adults or college students. Studying young adults can help provide added …

An Investigation Of Preferred Versus Imposed Exercise, Personality Traits, And Motivation On An Exercise Dependent College Aged Sample 2013, Chelsea M. Norton

Master's Theses

The purpose of this study was to: (a) investigate personality traits and motivation among an exercise dependent sample by using and examining theoretically based assessment tools and (b) measure feeling states under different types of physical activity among those who were considered to be exercise dependent. Four hundred twenty-three college students (54.4% male, 45.6% female) who met the inclusion criteria completed the Exercise Dependence Scale-Revised (EDS-R; Symons Downs, Hausenblas, & Nigg, 2004), Exercise Addiction Inventory (EAI; Terry, Szabó, & Griffiths, 2004), Exercise Identity Scale (EIS; Anderson & Cychosz, 1994), Behavioral Regulations in Exercise Questionnaire-2 (BREQ-2; Markland & Tobin, 2004), and …

The Influence Of Respiratory Muscle Training On Exercise Endurance 2013, Justin Vanderbeck

Master's Theses

Exercise endurance in multiple populations has been shown to increase after bouts of respiratory muscle training (RMT) (Bailey, Romer, Kelly, Wilkerson, Dimenna & Jones, 2010; Brown, Sharpe, Graham & Johnson, 2008; Griffiths & McConnell, 2007; Kilding, Brown & McConnell, 2007; Laoutaris et al., 2012; Markov, Spengler, Stuessi, Boutellier & Knöpfli-Lenzin, 2001; Spengler & Boutellier, 2000; Stuessi, Spengler, Knöpfli-Lenzin, Markov & Boutellier, 2001; Volianitis et al., 2000). The use of resistance via respiratory muscle strength training (RMST) has been shown to produce greater increases in endurance than with no resistance, but the method of RMST that produces the greatest increases in …

Resistance Training Behaviors In College-Aged Women 2013, Melanie Kornblatt

Master's Theses

The purpose of this study was to develop an understanding of women’s training behavior in the weight room. A secondary purpose was to determine what their beliefs and perceptions were in regards to lifting weights. Participants were college-aged females ranging in age from 17-29 years. Observations of weight lifting activities were conducted during peak hours of operation (Mondays, Wednesdays, and Fridays from 3- 6pm) in the fitness facility. A total of 60 hours of observations were conducted over a period of four weeks. In addition to observations, two focus groups were conducted. Participants were divided into two specific groups: I …

The Effect Of Skeletal Muscle Mass On Basal Metabolic Rate In College-Age Males 2013, Chase Vaughn

Master's Theses

There are many misconceptions within the fitness community. One such misconception is that an increase in muscle mass will have a large increase in basal metabolic rate, yet this may not be the best way for successful weight loss. The purpose of this study was to determine the strength of the correlation between skeletal muscle mass and basal metabolic rate. The hypothesis tested was that basal metabolic rate is strongly correlated with skeletal muscle mass. The participants were college-aged males (n =17). Skeletal muscle mass (SMM) was estimated using the skin-fold circumference model (SFCM): SMM (kg) = Ht (0.00744 CAG2 …

Determinants Of Food Insecurity Among Vulnerable White And Latino Households: Contextualizing The Impact Of Sociodemographic And Household-Level Factors, Alexandra Lund

Master's Theses

Household-level characteristics have been shown to be associated with food insecurity but studies among vulnerable populations are sparse. A food security assessment was developed to determine food security and collect sociodemographic and household level data across San Luis Obispo County. The assessments were administered to vulnerable groups through interviews at multiple sites across the County. Three household characteristics (marital status, number of children in the household and number of workers in the household) were examined in this analysis. A total of 808 surveys were collected, 69% in English and 31% in Spanish. Through ethnicity-stratified sequentially adjusted logistic regression models, the …