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Full-Text Articles in Life Sciences

Book Review: Habitantes De La Obscuridad (Fauna Ibero-Balear De Las Cuevas) – Inhabitants Of The Darkness (Ibero-Baleric Fauna Of Caves), Oana Teodora Moldovan Oct 2023

Book Review: Habitantes De La Obscuridad (Fauna Ibero-Balear De Las Cuevas) – Inhabitants Of The Darkness (Ibero-Baleric Fauna Of Caves), Oana Teodora Moldovan

International Journal of Speleology

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Book Review: Australian Caves And Karst Systems, John Webb, Susan White, Garry K. Smith, Jo De Waele Oct 2023

Book Review: Australian Caves And Karst Systems, John Webb, Susan White, Garry K. Smith, Jo De Waele

International Journal of Speleology

No abstract provided.


Why The Delay In Recognizing Terrestrial Obligate Cave Species In The Tropics?, Francis G. Howarth Feb 2023

Why The Delay In Recognizing Terrestrial Obligate Cave Species In The Tropics?, Francis G. Howarth

International Journal of Speleology

“Nothing could possibly live there!” They believed. Indeed, until recently, few specialized cave- adapted animals were known from volcanic, tropical, or oceanic island caves, and plausible theories had been put forward to explain their absence. But assume nothing in science! One must illuminate, explore, and survey habitats before declaring them barren. Our understanding of cave biology changed dramatically about 50 years ago following the serendipitous discovery of cave-adapted terrestrial arthropods in Brazil and on the young oceanic islands of the Galápagos and Hawai‘i. These discoveries and subsequent studies on the evolutionary ecology of cave animals have revealed a remarkable hidden …


Worldwide Distribution Of Cave-Dwelling Chelodesmidae (Diplopoda, Polydesmida), Rodrigo Salvador Bouzan, Jackson C. Means, Kaloyan Ivanov, Rodrigo L. Ferreira, Antonio Domingos Brescovit, Luiz Felipe Moretti Iniesta Dec 2022

Worldwide Distribution Of Cave-Dwelling Chelodesmidae (Diplopoda, Polydesmida), Rodrigo Salvador Bouzan, Jackson C. Means, Kaloyan Ivanov, Rodrigo L. Ferreira, Antonio Domingos Brescovit, Luiz Felipe Moretti Iniesta

International Journal of Speleology

Chelodesmidae is one of the most species rich families within the Myriapoda. However, little is known regarding their association with caves. We provide a list of all Chelodesmidae taxa reported from caves, map their worldwide distribution, and discuss the troglomorphic features of the group. A total of 25 species and subspecies from 20 genera and 2 subfamilies have been recorded from 59 caves and cave systems in 11 countries. These numbers represent a surprisingly modest proportion (~3%) of the approximately 800 described species in the family. Records of cave-dwelling chelodesmids appear to be geographically biased with most taxa reported from …


The Nitrogen Dynamics Of Deer Cave, Sarawak, And The Role Of Bat Caves As Biogeochemical Sinks In Tropical Moist Forests., Joyce Lundberg, Donald A. Mcfarlane, Guy Van Rentergem Nov 2022

The Nitrogen Dynamics Of Deer Cave, Sarawak, And The Role Of Bat Caves As Biogeochemical Sinks In Tropical Moist Forests., Joyce Lundberg, Donald A. Mcfarlane, Guy Van Rentergem

International Journal of Speleology

A better understanding of the role of bat caves as nitrogen sinks in tropical moist forest ecosystems can be expected to shed light on regional and spatial variability in nutrient recycling studies. We measured the nitrogen flux (in air and water) associated with a very large Chaerephon plicata bat colony in Deer Cave, Borneo, in the process generating a new, quantitative, estimate of the total bat population (774,828 ±48,320), and the first detailed modelling of an ammonia plume in a cave. Long-term storage of N does not occur in this wet cave. Our final budget numbers indicate that, of the …


Algae And Cyanobacteria In The Aphotic Habitats Of Veternica Cave (Medvednica Mt., Croatia) And Selected Caves Of The Dinaric Karst (South-Eastern Europe), Najla Baković, Tanja Pipan, Robert Baković, Roman Ozimec, Josip Rubinić, Renata Matoničkin Kepčija Nov 2022

Algae And Cyanobacteria In The Aphotic Habitats Of Veternica Cave (Medvednica Mt., Croatia) And Selected Caves Of The Dinaric Karst (South-Eastern Europe), Najla Baković, Tanja Pipan, Robert Baković, Roman Ozimec, Josip Rubinić, Renata Matoničkin Kepčija

International Journal of Speleology

Microphototrophs (algae and cyanobacteria) in karst environments have been intensively studied in aquatic epigean habitats. In recent decades knowledge about the communities inhabiting cave entrances and lampenflora has grown substantially, but the data about the communities in aphotic cave zone are scarce. This study aimed to investigate spatio-temporal presence of microphototrophs in the aphotic zone of Veternica Cave (Mt. Medvednica karst) and to present additional preliminary data from 22 caves of the Dinaric karst. The data were collected over ten years, in parallel with research on cave phagotrophic protists. In addition to the remains of microphototrophs, living algae and cyanobacteria …


Recreational Caving Impacts Of Visitors In A High-Altitude Cave In Bolivian Andes: Main Effects On Microhabitat Structure And Faunal Distribution, Lais Furtado Oliveira, Rodrigo L. Ferreira, Jaime Iván Rodríguez Fernández, Marconi Souza Silva Jul 2022

Recreational Caving Impacts Of Visitors In A High-Altitude Cave In Bolivian Andes: Main Effects On Microhabitat Structure And Faunal Distribution, Lais Furtado Oliveira, Rodrigo L. Ferreira, Jaime Iván Rodríguez Fernández, Marconi Souza Silva

International Journal of Speleology

The cave’s physical environment can be affected by tourism activities but only a few studies evaluated how recreational use may affect the cave fauna, mainly in caves with a low number of visitors per year. To test the hypothesis that recreational use led to changes in habitat structure and invertebrate diversity, distinct scales along a cave were analyzed. Distinct areas with and without human visitation were analyzed and transects (10 x 3 m) and quadrats (1 x 1 m) were used to access the invertebrate communities and environmental traits. Thirty-two invertebrate species were recorded, among which six are troglobitic. The …


Monitoring Photosynthetic Activity Using In Vivo Chlorophyll A Fluorescence In Microalgae And Cyanobacteria Biofilms In The Nerja Cave (Malaga, Spain), Yolanda Del Rosal, Juan Muñoz-Fernández, Paula S.M. Celis-Plá, Mariona Hernández-Mariné, Félix Álvarez-Gómez, Salvador Merino, Félix L. Figueroa Dec 2021

Monitoring Photosynthetic Activity Using In Vivo Chlorophyll A Fluorescence In Microalgae And Cyanobacteria Biofilms In The Nerja Cave (Malaga, Spain), Yolanda Del Rosal, Juan Muñoz-Fernández, Paula S.M. Celis-Plá, Mariona Hernández-Mariné, Félix Álvarez-Gómez, Salvador Merino, Félix L. Figueroa

International Journal of Speleology

The characterization of the most common photosynthetic biofilms in the Nerja Cave by the continuous monitoring of the in vivo chlorophyll a (Chl a) fluorescence and the incorporation of the irradiance as a new environmental variable related to previous studies in the cave, have allowed us to improve our knowledge about the photosynthetic pattern of the biofilms of the cave. Effective quantum yield (ΔF/Fm) and relative electron transport rate (rETR) were determined during periods of the light, whereas the maximal quantum yield (Fv /Fm) was determined during dark periods. Increases in …


Molecular Genetic Analysis Of Stygobiotic Shrimps Of The Genus Xiphocaridinella (Crustacea: Decapoda: Atyidae) Reveals A Connection Between Distant Caves In Central Abkhazia, Southwestern Caucasus, Ivan Marin, Ilya Turbanov Sep 2021

Molecular Genetic Analysis Of Stygobiotic Shrimps Of The Genus Xiphocaridinella (Crustacea: Decapoda: Atyidae) Reveals A Connection Between Distant Caves In Central Abkhazia, Southwestern Caucasus, Ivan Marin, Ilya Turbanov

International Journal of Speleology

Based on the morpho-genetic study of stygobiotic shrimps from the genus Xiphocaridinella Sadowsky, 1930 (Crustacea: Decapoda: Atyidae), a hydrogeological connection of a number of distant caves in Central Abkhazia of the southwestern Caucasus is satisfied, which indicates the possibility of using biospeleological studies in some cases to identify karst hydrosystems together with traditional hydrogeological methods. Moreover, a new stygobiotic atyid shrimp from the genus Xiphocaridinella, X. kelasuri sp. n., is described based on morphology and analysis of mitochondrial cytochrome oxidase I DNA sequences from three distant caves. The new species is genetically divergent from relatives and phylogenetically related to …


Do Different Relevance Attributes Indicate The Same Conservation Priorities? A Case Study In Caves Of Southeastern Brazil, Maysa F.V.R. Souza, Denizar A. Alvarenga, Marconi Souza-Silva, Rodrigo L. Ferreira Jul 2021

Do Different Relevance Attributes Indicate The Same Conservation Priorities? A Case Study In Caves Of Southeastern Brazil, Maysa F.V.R. Souza, Denizar A. Alvarenga, Marconi Souza-Silva, Rodrigo L. Ferreira

International Journal of Speleology

In the last decade, the scientific community brought to the debate gaps that slow down the advance of knowledge regarding global biodiversity. More recently, this discussion has reached subterranean environments, where these gaps are even more dramatic due to the relict and vulnerable nature of their species. In this context, we tested ecological metrics related to some of these gaps, checking if the biological relevance of the caves would change depending on ecological attributes related to each metric. The study was carried out in caves from southeastern Brazil, located in a region presenting a high richness of troglobitic species restricted …


Molecular Phylogeny Of Cave Dwelling Eremogryllodes Crickets (Orthoptera, Myrmecophilidae) Across Zagros Mountains And Southern Iran, Mohadeseh Sadat Tahami, Mina Hojat-Ansari, Anna Namyatova, Saber Sadeghi Jun 2021

Molecular Phylogeny Of Cave Dwelling Eremogryllodes Crickets (Orthoptera, Myrmecophilidae) Across Zagros Mountains And Southern Iran, Mohadeseh Sadat Tahami, Mina Hojat-Ansari, Anna Namyatova, Saber Sadeghi

International Journal of Speleology

Recently, several new species and subspecies from the genus Eremogryllodes Chopard, 1929 (Insecta: Orthoptera: Myrmecophilidae) inhabiting caves of Iran, have been described based on morphology. The high variation of genitalia structure along with high similarity of external morphology between populations hamper the precise species identification. Thus, molecular approaches are critical to determine the taxonomic positions of species/subspecies of this genus. Here we provide the molecular phylogeny, based on the 16S rRNA mitochondrial gene, of recently described species of Eremogryllodes along with some unidentified specimens from the same region. The results support the monophyly of the family Myrmecophilidae. The topology of …


Cave-Dwelling Heleomyzid Flies (Diptera: Heleomyzidae) From The Polish Caves. Historical Overview And New Data, Joanna Kocot-Zalewska, Andrzej J. Woźnica Jun 2021

Cave-Dwelling Heleomyzid Flies (Diptera: Heleomyzidae) From The Polish Caves. Historical Overview And New Data, Joanna Kocot-Zalewska, Andrzej J. Woźnica

International Journal of Speleology

This article details the analysed results of the recent study on heleomyzid flies (Diptera: Heleomyzidae) that occurred in the caves of Kraków-Częstochowa Upland. Additionally, all accessible information about those flies from the Polish caves has been compiled. In effect, 23 species from seven genera were identified. Among these determined species, one was considered as troglobiont, eleven as eutroglophiles, nine as subtroglophiles, and two as trogloxenes. Dominant species were Scoliocentra brachypterna, Heleomyza captiosa and Eccoptomera pallescens. The highest number of species has currently been found in the Kraków-Częstochowa Upland.


Microbially-Mediated Carbonate Dissolution And Precipitation; Towards A Protocol For Ex-Situ, Cave-Analogue Cultivation Experiments, Vanessa E. Johnston, Andrea Martín-Pérez, Sara Skok, Janez Mulec Apr 2021

Microbially-Mediated Carbonate Dissolution And Precipitation; Towards A Protocol For Ex-Situ, Cave-Analogue Cultivation Experiments, Vanessa E. Johnston, Andrea Martín-Pérez, Sara Skok, Janez Mulec

International Journal of Speleology

Subterranean calcite dissolution and precipitation are often considered as strictly geochemical processes. The active involvement of microbes in these processes is commonly underestimated in the literature due to general oligotrophic conditions in caves, except in particular cave conditions, such as sulfidic caves and moonmilk deposits, where the presence of microbes likely plays a key role in mineral deposition. Here, we study the possible involvement of microbes from Postojna Cave, Slovenia, in carbonate dissolution (litholysis) and precipitation (lithogenesis). Microbes were sampled from small pools below hydrologically diverse drip sites and incubated on polished limestone tablets at 10 and 20°C for 2 …


Bacteria, Guano And Soot: Source Assessment Of Organic Matter Preserved In Black Laminae In Stalagmites From Caves Of The Sierra De Atapuerca (N Spain), Joeri Kaal, Virginia Martínez-Pillado, Antonio Martínez Cortizas, Jorge Sanjurjo Sánchez, Arantza Aranburu, Juan-Luis Arsuaga, Eneko Iriarte Apr 2021

Bacteria, Guano And Soot: Source Assessment Of Organic Matter Preserved In Black Laminae In Stalagmites From Caves Of The Sierra De Atapuerca (N Spain), Joeri Kaal, Virginia Martínez-Pillado, Antonio Martínez Cortizas, Jorge Sanjurjo Sánchez, Arantza Aranburu, Juan-Luis Arsuaga, Eneko Iriarte

International Journal of Speleology

Speleothems are a recognized source of paleoclimatic information, but their value as a source of signals from human activities in caves with an archaeological record has rarely been explored. Previous studies of speleothems in the Sierra de Atapuerca karst system (Burgos, northern Spain) revealed an important human fossil record, provided information about human activities in and around these caves, and the impacts on their natural environment. The present study reports the results of molecular characterization of dark-colored laminae from the stalagmites Ilargi (Galería de las Estatuas) and GS1, GS2, and GS3 (Galería del Silo), by pyrolysis-GC-MS (Py-GC-MS) and …


Ecophysiological Responses Of Two Closely Related Epigean And Hypogean Niphargus Species To Hypoxia And Increased Temperature: Do They Differ?, Tatjana Simčič, Boris Sket Apr 2021

Ecophysiological Responses Of Two Closely Related Epigean And Hypogean Niphargus Species To Hypoxia And Increased Temperature: Do They Differ?, Tatjana Simčič, Boris Sket

International Journal of Speleology

Ecological performance of animals depends on physiological and biochemical processes that are adjusted to the environment. The responses to hypoxia or anoxia have been frequently studied in subterranean aquatic organisms in order to find potential adaptations to restrict oxygen conditions occurring in the underground habitats. However, some previous studies have compared phylogenetic distant epigean and hypogean species or the epigean and hypogean populations of the same species due to little chance to compare closely related epigean and hypogean species. Therefore, in this study, we compared the effects of exposure to hypoxia, followed by reoxygenation, and increased temperature on oxygen consumption, …


Protura (Arthropoda: Hexapoda) In Slovenian Caves, Loris Galli, Franc Janžekovič, Peter Kozel, Tone Novak Mar 2021

Protura (Arthropoda: Hexapoda) In Slovenian Caves, Loris Galli, Franc Janžekovič, Peter Kozel, Tone Novak

International Journal of Speleology

Protura is a small class of Hexapoda, generally poorly known, and rather scarce data on its occurrence in caves are scattered throughout literature on fauna in caves and in some papers on Protura. Although the cave-dwelling fauna is relatively well studied in Slovenia, published records on Protura are rare. In this paper, data on the occurrence and abundance of Protura in Slovenian caves are considered. Various statistical analyses were performed to compare caves with Protura to those without in 60 intensively monitored cavities to detect any differences in the selected environmental conditions. No significant difference was obtained. Samples collected from …


Microhabitat Selection Of A Sicilian Subterranean Woodlouse And Its Implications For Cave Management, Giuseppe Nicolosi, Stefano Mammola, Salvatore Costanzo, Giorgio Sabella, Rosolino Cirrincione, Giovanni Signorello, Marco Isaia Mar 2021

Microhabitat Selection Of A Sicilian Subterranean Woodlouse And Its Implications For Cave Management, Giuseppe Nicolosi, Stefano Mammola, Salvatore Costanzo, Giorgio Sabella, Rosolino Cirrincione, Giovanni Signorello, Marco Isaia

International Journal of Speleology

Human activities in subterranean environments can affect different ecosystem components, including the resident fauna. Subterranean terrestrial invertebrates are particularly sensitive to environmental change, especially microclimatic variations. For instance, microclimate modifications caused by the visitors may directly affect local fauna in caves opened to the public. However, since numerous factors act synergistically in modulating the distribution and abundance of subterranean species, it remains challenging to differentiate the impact of human intervention from that of other factors. Therefore, evidence of the impact of tourism on cave invertebrate fauna remains scarce. Over a year and with approximately two visits a month, we investigated …


Microbiological Study In A Gneissic Cave From Sri Lanka, With Special Focus On Potential Antimicrobial Activities, Ethige Isuru P. Silva, Pathmakumara Jayasingha, Saman Senanayake, Anura Dandeniya, Dona Helani Munasinghe Mar 2021

Microbiological Study In A Gneissic Cave From Sri Lanka, With Special Focus On Potential Antimicrobial Activities, Ethige Isuru P. Silva, Pathmakumara Jayasingha, Saman Senanayake, Anura Dandeniya, Dona Helani Munasinghe

International Journal of Speleology

The emergence of antibiotic resistance is a global health crisis, thus the search for novel antimicrobial compounds has become a continuous necessity. Underexplored and extreme environments, such as cave ecosystems, have been identified as a promising potential source for the discovery of novel microorganisms with novel antimicrobial compounds (AMC). This study presents the first cave microbiological investigation in Sri Lanka, with a special preference for bioprospecting of novel AMC. The cave sediment characterization demonstrated the presence of close to strong acidic conditions (pH 3.1 – 3.3) and thus indicates the possibility of isolating acidophilic microorganisms. Eight cave wall/ceiling fungal strains …


Increased Cave Use By Butterflies And Moths: A Response To Climate Warming?, Otto Moog, Erhard Christian, Rudolf Eis Jan 2021

Increased Cave Use By Butterflies And Moths: A Response To Climate Warming?, Otto Moog, Erhard Christian, Rudolf Eis

International Journal of Speleology

Between 2015 and 2019, the list of Lepidoptera from “cave” habitats (i.e., proper caves, rock shelters and artificial subterranean structures) in Austria grew from 17 to 62 species, although the effort of data collection remained nearly constant from the late 1970s onwards. The newly recorded moths and butterflies were resting in caves during daytime in the the warm season, three species were also overwintering there. We observed Catocala elocata at 28 cave inspections, followed by Mormo maura (18), Catocala nupta (7), Peribatodes rhomboidaria, and Euplagia quadripunctaria (6). More than half of the species have been repeatedly observed in caves …


Stygobitic Crustaceans In An Anchialine Cave With An Archeological Heritage At Vodeni Rat (Island Of Sveti Klement, Hvar, Croatia), Alessandro Montanari, Nicolas Cerveau, Barbara Fiasca, Jean-François Flot, Diana Galassi, Maurizio Mainiero, David Mcgee, Tadeusz Namiotko, Stefano Recanatini, Fabio Stoch Nov 2020

Stygobitic Crustaceans In An Anchialine Cave With An Archeological Heritage At Vodeni Rat (Island Of Sveti Klement, Hvar, Croatia), Alessandro Montanari, Nicolas Cerveau, Barbara Fiasca, Jean-François Flot, Diana Galassi, Maurizio Mainiero, David Mcgee, Tadeusz Namiotko, Stefano Recanatini, Fabio Stoch

International Journal of Speleology

A group of four amphoras found in the anchialine cave of Vodeni Rat in the Croatian island of Sveti Klement, on a rocky ledge at 24 m water depth, indicates that a freshwater source was exploited at the bottom of this karstic pit by islanders and/or passing-by sailors from the late Roman Republican Period to the Early Medieval Period. In other words, prior to the 4th–7th century CE, Vodeni Rat was not an anchialine cave but a Pleistocene karstic pit with a freshwater pool at the bottom. Seawater started to infiltrate this cavity via newly opened fissures …


Distribution Of Crickets (Subfamily: Phalangopsinae) In Caves Of Baratang Island, Andaman And Nicobar Islands, India, Amruta Dhamorikar, Dhanusha Kawalkar, Shirish Manchi Oct 2020

Distribution Of Crickets (Subfamily: Phalangopsinae) In Caves Of Baratang Island, Andaman And Nicobar Islands, India, Amruta Dhamorikar, Dhanusha Kawalkar, Shirish Manchi

International Journal of Speleology

Cave-dwelling organisms share different ecological and evolutionary relationships with caves. Based on these interactions, they are categorized as troglobites, troglophiles, and trogloxenes. In India, caves are meagerly explored, and thus cave study is in its infancy in India. Through the present study, we attempted to understand and model the distribution of crickets (Family Phalangopsidae), a critical group of insects - being the primary consumers in the cave ecosystems. We sampled seven caves using belt transects (N = 184; total area covered = 1294.9 m2) with 1 m width. During the survey, we encountered 818 individual crickets (116.85 ± …


The Impact Of Sample Processing And Media Chemistry On The Culturable Diversity Of Bacteria Isolated From A Cave, Katey E. Bender, Katelyn Glover, Alexander Archey, Hazel A. Barton Oct 2020

The Impact Of Sample Processing And Media Chemistry On The Culturable Diversity Of Bacteria Isolated From A Cave, Katey E. Bender, Katelyn Glover, Alexander Archey, Hazel A. Barton

International Journal of Speleology

Although molecular approaches can identify members of microbial communities in the environment, genomic information does not necessarily correlate with environmental phenotype. Understanding functional roles can be done by cultivating representative species, yet the culturablility of bacteria from caves remains low, at 0.02%, limiting our understanding of microbial community interactions and processes. We have investigated several factors influencing culturability of bacteria from a single sample location in Maxwelton Sink Cave, WV, USA. Extended incubation of inoculated plates showed a significant increase in colony counts from two to four weeks, indicating that extended incubations increase culturability. There were no significant differences in …


The Role Of Microhabitats In Structuring Cave Invertebrate Communities In Guatemala, Gabrielle S.M. Pacheco, Marconi Souza Silva, Enio Cano, Rodrigo L. Ferreira Sep 2020

The Role Of Microhabitats In Structuring Cave Invertebrate Communities In Guatemala, Gabrielle S.M. Pacheco, Marconi Souza Silva, Enio Cano, Rodrigo L. Ferreira

International Journal of Speleology

Several studies have tried to elucidate the main environmental features driving invertebrate community structure in cave environments. They found that many factors influence the community structure, but rarely focused on how substrate types and heterogeneity might shape these communities. Therefore, the objective of this study was to assess which substrate features and whether or not substrate heterogeneity determines the invertebrate community structure (species richness and composition) in a set of limestone caves in Guatemala. We hypothesized that the troglobitic fauna responds differently to habitat structure regarding species richness and composition than non-troglobitic fauna because they are more specialized to live …


Did You Wash Your Caving Suit? Cavers’ Role In The Potential Spread Of Pseudogymnoascus Destructans, The Causative Agent Of White-Nose Disease, Violeta Zhelyazkova, Antonia Hubancheva, Georgi Radoslavov, Nia Toshkova, Sebastien J. Puechmaille Sep 2020

Did You Wash Your Caving Suit? Cavers’ Role In The Potential Spread Of Pseudogymnoascus Destructans, The Causative Agent Of White-Nose Disease, Violeta Zhelyazkova, Antonia Hubancheva, Georgi Radoslavov, Nia Toshkova, Sebastien J. Puechmaille

International Journal of Speleology

White-Nose Disease (WND) has killed millions of hibernating bats in the US and Canada. Its causative agent, the fungus Pseudogymnoascus destructans was introduced to North America, but is native to Europe and Asia, where it is not associated with mass mortality. Although it is nearly impossible to eradicate an emerging wildlife disease, research on P. destructans spread mechanisms can aid in prevention of new introductions and development of better environmental management strategies. It is of particular importance to quantify the potential role of people visiting caves (cavers, tourists, bat researchers, etc.) whom inadvertently move P. destructans spores between sites, and …


Anchialine Biodiversity In The Turks And Caicos Islands: New Discoveries And Current Faunal Composition, Brett C. Gonzalez, Alejandro Martínez, Jørgen Olesen, Sarit B. Truskey, Lauren Ballou, Marc Allentoft-Larsen, Joost Daniels, Paul Heinerth, Mark Parrish, Naqqi Manco, Jon Ward, Thomas M. Iliffe, Karen J. Osborn, Katrine Worsaae May 2020

Anchialine Biodiversity In The Turks And Caicos Islands: New Discoveries And Current Faunal Composition, Brett C. Gonzalez, Alejandro Martínez, Jørgen Olesen, Sarit B. Truskey, Lauren Ballou, Marc Allentoft-Larsen, Joost Daniels, Paul Heinerth, Mark Parrish, Naqqi Manco, Jon Ward, Thomas M. Iliffe, Karen J. Osborn, Katrine Worsaae

International Journal of Speleology

Lying at the southernmost point of the Lucayan Archipelago, the Turks and Caicos Islands are amongst the better studied localities for anchialine cave biodiversity. For nearly five decades, novel invertebrate fauna, comprised primarily of crustaceans, have been collected from these tidally influenced pools – but new findings are always on the horizon. Herein we present new records of crustaceans and annelids from anchialine blue holes and horizontal caves of the Turks and Caicos. These findings include two potentially new species of meiofaunal annelids and a new species of remipede collected from a shallow water cave pool. Our 2019 expedition additionally …


Culture Dependent Analysis Of Bacterial Diversity In Canada’S Raspberry Rising Cave Revealed Antimicrobial Properties, Soumya Ghosh, Gabrielle Kam, Monique Nijjer, Christian Stenner, Naowarat Cheeptham Mar 2020

Culture Dependent Analysis Of Bacterial Diversity In Canada’S Raspberry Rising Cave Revealed Antimicrobial Properties, Soumya Ghosh, Gabrielle Kam, Monique Nijjer, Christian Stenner, Naowarat Cheeptham

International Journal of Speleology

Bacteria and archaea thrive in terrestrial subsurface environments because of their unique physiology. Over time, these unique microorganisms may have adapted to possess specialized metabolic pathways that sustain their continued existence in caves, one of harshest environments on earth. The present study elucidates cultivation based microbial diversity of the cave sediments and wall scrapings collected from seven different locations in Raspberry Rising Cave located in the Columbia Mountain Range, British Columbia, Canada. A total of 103 cultivable bacteria from the cave were isolated on various agar media including R2A, Hickey-Tresner, and DifcoTM Actinomycetes Isolation agar media. Taxonomical phylogenetic analysis …


Impacts Of Invasive Rats On Hawaiian Cave Resources, Francis G. Howarth, Fred D. Stone Feb 2020

Impacts Of Invasive Rats On Hawaiian Cave Resources, Francis G. Howarth, Fred D. Stone

International Journal of Speleology

Although there are no published studies and limited data documenting damage by rodents in Hawaiian caves, our incidental observations during more than 40 years of surveying caves indicate that introduced rodents, especially the roof rat, Rattus rattus, pose significant threats to vulnerable cave resources. Caves, with their nearly constant and predictable physical environment often house important natural and cultural features including biological, paleontological, geological, climatic, mineralogical, cultural, and archaeological resources. All four invasive rodents in Hawai‘i commonly nest in cave entrances and rock shelters, but only the roof rat (Rattus rattus) habitually enters caves and utilizes areas …


Active Growth Of Non-Hydrothermal Subaqueous And Subaerial Barite (Baso4) Speleothems In Lechuguilla Cave (New Mexico, Usa), Max Wisshak, Hazel A. Barton, Katey E. Bender, Harvey R. Duchene Jan 2020

Active Growth Of Non-Hydrothermal Subaqueous And Subaerial Barite (Baso4) Speleothems In Lechuguilla Cave (New Mexico, Usa), Max Wisshak, Hazel A. Barton, Katey E. Bender, Harvey R. Duchene

International Journal of Speleology

Barite (BaSO4) speleothems have been reported from caves around the globe and interpreted to have chiefly formed in phreatic, hypogene, hydrothermal settings. Here we report two contrasting types of barite speleothems (bluish tabular crystals in a shallow pool and actively dripping greenish stalactites), which today form at lower temperatures in the non-hydrothermal and vadose environment of Lechuguilla Cave, New Mexico, USA. Scanning electron microscopy analysis, along with energy- and wavelength-dispersive X-ray spectroscopy (EDS, WDS), as well as X-ray diffraction (XRD), characterize the habit and chemical composition as barite. Fractionation of the minor element calcium is related to growth …


Survey Of Histoplasma Capsulatum In Bat Guano And Status Of Histoplasmosis In Slovenia, Central Europe, Janez Mulec, Saša Simčič, Tadeja Kotar, Romina Kofol, Sanja Stopinšek Jan 2020

Survey Of Histoplasma Capsulatum In Bat Guano And Status Of Histoplasmosis In Slovenia, Central Europe, Janez Mulec, Saša Simčič, Tadeja Kotar, Romina Kofol, Sanja Stopinšek

International Journal of Speleology

There have been increasing reports on the presence of Histoplasma capsulatum in some European countries. The study investigated the presence of Histoplasma in bat guanos, speleologists with records of visiting Histoplasma-endemic regions and patients with histoplasmosis. A commercial ALPHA Histoplasma Antigen enzyme immunoassay was tested as an alternative methodology to detect Histoplasma in environment and compared with polymerase chain reaction (PCR) assays. The presence of Histoplasma antigen in bat guanos was not confirmed by PCR. Among 14 healthy speleologists, two were indicated as having the Histoplasma antigen in urine, but expressed negative PCR-specific results for the Histoplasma antigen. Five …


Screening The Cultivable Cave Microbial Mats For The Production Of Antimicrobial Compounds And Antibiotic Resistance, Jerneja Ambrožič Avguštin, Patricia Petrič, Lejla Pašić Dec 2019

Screening The Cultivable Cave Microbial Mats For The Production Of Antimicrobial Compounds And Antibiotic Resistance, Jerneja Ambrožič Avguštin, Patricia Petrič, Lejla Pašić

International Journal of Speleology

The current work extends the phenotypic characterization of a bacterial culture collection obtained from white, yellow, grey or pink microbial cave wall colonies that are common in the caves of Slovenian Karst. We have determined antibiotic resistance to 22 natural and synthetic antibiotics in 69 isolates from the microbial mats. Thirty-eight isolates (52%) were resistant to 1-5 antibiotics; another 27 isolates (37%) were resistant to 6-10 antibiotics; and 7 isolates (0.1%) were resistant to 11-17 antibiotics. We screened for production of antimicrobial compounds by growing cave isolates on five different media and overlaying individual cultures with ten Gram-positive and Gram …