Life Sciences Commons^™

The Effect Of Clay On The Inhibitory Effect Of Mustard Seed Meal On Velvetleaf Seedling Growth, Heeyun Oh

Honors Theses

Mustard seed meal (MSM) has been studied as a biofumigant in suppressing weed growth in an organic-friendly way. Previous studies have shown that MSM in the presence of elevated sand content was highly effective in suppressing the germination of velvetleaf seeds, and that as clay/silt in sand content increased, MSM effectiveness decreased in suppressing seed germination. Based on these results, it was postulated that clay may have effects on MSM by inhibiting this biofumigant from suppressing weed growth. Two different types of pure clay--kaolin and montmorillonite--were selected with their increasing concentrations. Low concentrations of clay do not seem to influence …

Reassessing The Parameters Of Phonotaxis In Female Acheta Domesticus, Jewel Murray

Honors Theses

To study phonotaxis in crickets a trackball system has been traditionally used, which consists of a spherical, air supported styrofoam ball placed in a soundproof container in which crickets are tethered to. Two computer mice detect its movement. A speaker is at one end of the container, and it is connected to a computer which generates calls with pre-set parameters. Once a cricket is attached to the trackball system, it can freely navigate toward the source of the sound, while the styrofoam ball remains stationary.

This apparatus has been used to measure the walking distance and angular orientation. When evaluating …

Phonotactic Tuning In Female Cricket Acheta Domesticus, Jessica Rim

Honors Theses

Phonotactic behavior of female cricket Acheta domesticus has been shown to vary among individuals. While some females are finely tuned to calls with syllable periods in the natural range of conspecific males, others respond phonotactically to a wider range of syllable periods and therefore lack the ability to discriminate between attractive and unattractive calls. When females are exposed to males but prevented from mating, their ability to discriminate attractive calls is reduced, suggesting that factors other than mating alter phonotactic behavior. This study evaluated the effect of male exposure on females’ phonotactic tuning and responsiveness in relation to underlying neural …

The Role Of The Prodomain In The Folding Of Carboxypeptidase A1, Masy Domecillo

Honors Theses

Carboxypeptidase A1 (CPA1) is an enzyme utilized in digestion. CPA1 contains a prodomain region, which must be cleaved for enzymatic function. The prodomain region has been hypothesized to be necessary for structural stability, as previous studies were unable to produce a functional CPA1 protein without the prodomain region. However, the recent discovery of a related enzyme, Carboxypeptidase 0 (CP0), which does not have a prodomain yet functions enzymatically, challenges this assertion. To determine if the prodomain is essential to the folding of CPA1, an HA-tag was added for detection, and the prodomain was deleted via PCR. A Western blot was …

Analysis Of Invasion Proteins Mmp2, Mmp9, Adam12, And Adam17 In Glioblastoma U87mg Cells Treated With Anti-Cancer Compound 3,4-Dimethoxybenzaldehyde, Andras Muranyi

Honors Theses

This research tested the effectiveness of novel compound 3,4-dimethoxybenzaldehyde, demonstrated to have anti-cancer properties. U87MG Glioblastoma cells were exposed to the compound at its LC50 concentration, then processed to collect proteins from the cells. Proteins were analyzed via Western blotting for specific protein levels of matrix metalloproteinase 2 and 9 (MMP) and disintegrin and metalloprotease 12 and 17 (ADAM). Previous research indicates these proteins are involved in the invasive properties of glioblastoma cells. Westerns were quantified with ImageJ and compared using a one-way ANOVA. Results indicate the compound has minimal effect upon the expression of MMP2, MMP9, ADAM12, ADAM17 proteins.

Phonotactic Tuning In Female Cricket Acheta Domesticus, Jessica Rim

Honors Theses

Phonotactic behavior of female cricket Acheta domesticus has been shown to vary among individuals. While some females are finely tuned to calls with syllable periods in the natural range of conspecific males, others respond phonotactically to a wider range of syllable periods and therefore lack the ability to discriminate between attractive and unattractive calls. When females are exposed to males but prevented from mating, their ability to discriminate attractive calls is reduced, suggesting that factors other than mating alter phonotactic behavior.

This study evaluated the effect of male exposure on females’ phonotactic tuning and responsiveness in relation to underlying neural …

Attempted Synthesis & Antibacterial Properties Of Apt-6k Against Ndm-1 K. Pneumoniae, Alec Bofetiado

Honors Theses

NDM-1 K. pneumoniae is a highly resistant bacterial organism that is capable of causing debilitating nosocomial infections in immunocompromised patients. Only "last-resort" antibiotics--such as colistin--work against this organism. Therefore, new antibiotics are needed to help fight against these types of infections. APT-6K is a novel compound that was demonstrated to be effective against MRSA with nanomolar concentrations in a prior study. Novel methods of APT-6K synthesis and its testing for antibiotic effects against NDM-1 K. pneumoniae were attempted in this research. APT-6K synthesis was unsuccessful. Commercially-prepared APT-6K also did not demonstrate growth inhibition against NDM-1 K. pneumoniae nor against a …

Monitoring The Immune Status Of Calves At The Agricultural Education Center, Jared Wallen

Honors Theses

Each year at the Andrews University Agriculture Education Center calves born to the resident cows and calves that are purchased are raised by the students in the animal science program. In each of the previous two years that calves have been raised, morbidities and mortalities have been observed. In order to prevent any calves from dying and reduce the rate of illness this research project was designed to monitor the calves’ immune status. By doing weekly blood draws, the total serum protein levels were determined and used as a standard for their health. Body temperature, food and water intake, and …

Finding Optimal Opa-Iblc Derivatized Amino Acid Analysis Parameters With Three-Dimensional Fluorometric Scans For Hplc Method Validation, Aaron Jacobs

Honors Theses

Three-dimensional fluorometric scans of OPA-IBLC derivatized glutamic acid and histidine solutions revealed that decreasing concentration maintained maximum λem but produced different maximum λex. In pH 0.4 M sodium borate buffer (pH 10.4), 50 μM derivatized amino acid solutions had maxima around 350- to 450-nm λex-λem where 12.5 μM solutions had maxima around 230- to 450-nm λex- λem. In the lower concentration, the λex peak at 230 nm was about 80% higher than 350 nm. Thus, collecting spectra with 230 nm λex would provide better LOD and LOQ. Results inform HPLC standard concentrations since differential quenching effects may produce non-linear calibration …

Thermodynamic Analysis Of Pamam Dendrimer Synthesis, Ryutaro Einar Jacobson

Honors Theses

The objective of this research was to determine kinetic and thermodynamic data about the reaction between ethylene diamine and methyl acrylate which is used in the production of PAMAM dendrimers. Establishing a rate law and finding values such as rate constant, activation energy, and Arrhenius coefficient will help in determining the best possible conditions for the reaction. A rate law was established through the method of initial rates, comparing the rates of reactions with different initial concentrations to one another. Additional reactions were run with active cooling to observe the effects on rate and reaction progress, and finally reactions were …

Enamel Corrosion Levels On Squirrel Teeth From The Fox Canyon Locality, Pliocene Of Kansas, Emma Tennyson

Honors Theses

The enamel of teeth may be corroded after death, especially if the teeth pass through the digestive tract of a predator with low gastric pH. This study characterized enamel corrosion of fossil squirrel teeth from two species (Otospermophilus rexroadensis and Ictidomys meadensis) obtained from the Fox Canyon locality of Kansas. Using microscopic imaging, I categorized teeth based on corrosion level. Overall, 45% of specimens exhibited heavy to extreme corrosion. There were no significant differences in the distribution of corrosion categories between both upper and lower teeth of either species (O. rexroadensis: X2 (3, N = 140) = .562, p= .905; …

Evaluation Of Phonotactic Behavior In Male-Exposed Female Cricket Acheta Domesticus, Janelle An

Honors Theses

Phonotaxis is defined as the movement of organisms in response to an auditory stimulus. It plays an important role in helping animals find a potential mate, and thus it is worthwhile to understand what factors may influence phonotaxis. Phonotactic behavior in virgin female cricket Acheta domesticus has been the focus of multiple studies, which have reported a degree of variability among individuals. Several factors that have been reported to influence such variability include age, environmental temperature, and the presence of certain neuromodulators. This study investigated possible changes in the behavioral responses of young females when raised with males but not …

Modulation Of Apoptosis In Breast Cancer Cells Mda-Mb-157, 93a And 93b By Aqueous Extract Of Chinese Medical Herb Scutellaria Barbata, Nathaly Manrique

Honors Theses

Scutellaria barbata (S. barbata), a Chinese herb used in Traditional Chinese Medicine has been known to contain anti-cancer properties. SB has been investigated as a potential treatment for multiple types of cancer including colon cancer, glioblastoma, and ovarian cancer. Breast cancer cell lines MDA-MB-157 and its APC-mutants 93A and 93B are more resistant to treatment due to genetic modifications. In this study, the effectiveness of S. barbata in apoptotic modulation of breast cancer cell lines MDA-MB-157, 93A, and 93B was investigated. Assessments were performed using green/red/blue fluorescent Apoptosis/Necrosis Detection Kit and the Human Apoptosis Antibody Array - Membrane (43 Targets) …

Comparison Of Enamel Microstructure Of Ictidomys Tridecemlineatus Formed During Hibernation Versus The Active Season, Amanda Cho

Honors Theses

Ground squirrel incisors grow continuously, preserving a record of their most recent weeks of life. Previous research demonstrated that an abnormality in the surface of incisor enamel and dentin of thirteen-lined ground squirrels (Ictidomys tridecemlineatus) corresponded with hibernation. Using scanning electron microscopy, we compared internal microstructure of incisor enamel deposited during and outside of hibernation to determine if surface disruptions corresponded to differences in internal microstructure. For one specimen, hibernation enamel displayed irregularities in microstructure that were not present in non-hibernation enamel, but this difference was not observed in other specimens. Given these inconclusive results, further research is warranted.

The Effects Of The Omega-3 Fatty Acids Docosahexaenoic Acid On Inm1 Gene Expression In Saccharomyces Cerevisiae, Haley Kang

Honors Theses

Bipolar disorder is a mental disorder associated with abnormal inositol levels. Preliminary studies show omega-3 fatty acids alter intracellular inositol levels; however, the cellular mechanism behind this process is unknown. Therefore, the focus of this study is to determine the effects of the omega-3 fatty acid docosahexaenoic acid (DHA) on the expression of INM1 which encodes inositol monophosphatase. Using yeast as our model system, cells were grown in varying concentrations DHA and the expression on INM1 was determined by RT-qPCR. The results show INM1 expression increased with increasing concentrations of DHA; thus, the effects of DHA on inositol levels may …

Novel Heterocyclic Arylidene Derivatives As Anticancer Agents Against U87 Human Glioblastoma, Benjamin H. Hiramoto

Honors Theses

The primary objectives of this interdisciplinary study were the synthesis of novel heterocyclic arylidenes and the investigation of their anticancer activity against U87 glioblastoma cell viability. Recently, novel hybrid derivatives have been considered as potential candidates for treating glioblastoma, demonstrating a synergistic anticancer effect in previous studies. 12 heterocyclic arylidenes with various functional groups, including halogens and boronic acid, were produced via a Knoevenagel condensation. These compounds and their starting reagents were then administered to U87 glioblastoma cancer cells at graded concentrations within a 12-well cell viability assay to determine each compound’s lethal concentration 50 (LC50). The LC50 of each …

Are Florida Manatees (Trichechus Manatus Latirostris) Wearing Their Teeth Beyond Functionality? Interspecific And Intraspecific Mesowear In Manatees, Nina Woodard

Honors Theses

Manatees (Trichechus spp.) are herbivorous aquatic mammals found in tropical and subtropical waters. At maturity, they possess only supernumerary molars (SM), with 5-8 in occlusion at each quadrant. Manatees exhibit a dental replacement system in which they shed old teeth anteriorly and erupt new teeth posteriorly. This adaptation is thought to have arisen to deal with abrasive foods. Mesowear (facet development on occlusal surfaces of teeth) increases from posterior (younger) to anterior (older) molars. Tooth functionality is linked to level of mesowear, with increased amounts resulting in decreased food-processing ability. Less functional teeth can result in an increase in feeding …

Synthesis Of An Arylidene Pyrazolone And Its Effects On Glioblastoma Cancer Cells, Joanna Deonarine

Honors Theses

The purpose of this research is to develop a new technique to synthesize a heterocyclic arylidene compound using fruit puree catalysis and investigate its anti-cancer activity on glioblastoma cancer cells. Recently, there has been interest in heterocycles due to their anticancer properties and they have been used in almost two-thirds of the novel molecular anticancer agents. Varying fruit purees were used to synthesize a novel hybrid arylidene pyrazolone from 3-methyl-1-phenyl-5-pyrazolone and 4-dimethylaminobenzaldehyde by modifying methodology developed by Dr. Murray. This arylidene pyrazolone was then tested for its anticancer activity on glioblastoma brain cancer cells using Dr. Smith’s 12-well cell viability …

Evaluation Of Anticancer Activity Of Heterocyclic Arylidenes On The U87mg Cancer Cell Line, Bernadette Flores

Honors Theses

Glioblastoma multiforme, is a type of brain cancer that develops from glial cells, which surround neurons and provide support and insulation. Previous investigation has shown that some heterocyclic compounds are key in improving the properties of anticancer drugs by enhancing lipophilicity, polarity, and other varying physiochemical features. Synthetic heterocyclic compounds used as anticancer drugs attempt to imitate naturally-occurring ligands and substrates so as to disturb the natural balance in cells. Testing was done to determine the anticancer abilities of hybrid compounds, heterocyclic arylidenes, containing various functional groups, including boronic acids, through a three-day testing process. These compounds were previous synthesized …

Synthesis Of Novel Temozolomide-Fatty Acid Imide Hybrid Compounds For The Chemotherapeutic Treatment Of Glioblastoma Multiforme, Janice Pakkianathan

Honors Theses

Glioblastoma multiforme (GBM) is an aggressive form of brain cancer that originates from glial cells, which make up the supportive tissue surrounding neurons. Temozolomide (TMZ) is the current chemotherapeutic drug administered to treat GBM as it works to inhibit the growth of the cancer cells. This research study focused on developing a method for synthesizing novel hybrid compounds that combines TMZ with various fatty acids known to have anticancer properties, forming a series of imide compounds with potential chemotherapeutic effects. Before synthesizing the compounds, various methods for synthesizing an imide from a primary amide were tested to ensure that TMZ …

Exploring Soil Texture Effects On Mustard Seed Meal Suppression Of The Weed Velvetleaf, Daheun Danielle Harning

Honors Theses

The use of mustard seed meal (MSM) as a biofumigant in managing weeds in agricultural settings has been well documented. Previous work with MSM has shown that soils with a higher sand content were better at weed suppression than soils with lower amounts of sand. It was hypothesized that the elevated sand content created more air space for volatile inhibitory compounds to diffuse and inhibit seed germination and growth. Although initial results from bioassays systematically testing the comparison of 5 differing levels of sand showed support for this hypothesis, further testing with micron-sized glass beads showed that loss of airspace …

Is Natural Selection Shaping Florida Manatees? : An Investigation Into The Body Shapes Between The Subspecies Of The West Indian Manatee, Juliane Johnson

Honors Theses

The West Indian manatee (Trichechus manatus) is currently divided into two subspecies: the Antillean (T. m. manatus) and Florida manatee (T. m. latirostris). Florida manatees are found at the northern extreme of the species range, primarily inhabiting the waters around the Florida peninsula, while the Antillean manatee inhabits the remaining range from the Caribbean islands to eastern Brazil. Only in the range of the Florida manatee does water temperature drop below 20°C during the winter, and these manatees have to seek warm water refuge for thermal regulation. Allen's rule predicts that in colder climates animals will adapt to have smaller …

Design And Synthesis Of A Novel Lsoxazoline As A Potential Pam-Agonist, Josselyn Roosenberg

Honors Theses

Progression of Alzheimer's disease is associated with a loss of Ml receptor activation in the brain. However, a lack of clinical success following attempts to activate the Ml receptor at the orthosteric site has contributed to a transition towards the allosteric pocket of the receptor. Here, positive allosteric modulators (PAMs) interact to potentiate the acetylcholine response. Recent research proposes that optimization of PAM activity at the expense of intrinsic agonism may posit a means to limit adverse side effects. Therefore, this project proposes a design and synthesis of a novel isoxazoline as a potent PAM agent with weak intrinsic agonism.

Analysis Of Dl-Amino Acid Ratios In Eggshells Using Reverse Phase-High Pressure Liquid Chromatography, Gergana Milkova

Honors Theses

HPLC methodology was developed to determine the concentrations and ratios of D to L amino acids in emu and ostrich eggshells treated with heat at different temperatures . We aimed to determine an internal standard and how ratios were affected under different conditions. An HPLC method was determined that gave consistent retention times and satisfactory enantioseparation . Calibration curves for each amino acid were developed from single and multi-amino acid containing dilution series producing a model that most closely replicates the eggshell-extracted amino acids.

Isolation And Characterization Of Novel Arginine-Derived Heterocyclic Amines, Nathaniel Srikureja

Honors Theses

Most known heterocyclic amines (HCAs) are potent mutagens present at significant concentrations in cooked meats, and can be synthesized from burned mixtures of L-creatin(in)e with various amino acids. We hypothesize that novel mutagenic HCAs may be synthesized from a heated plant-based food simulating binary amino acid mL'\:ture: L-arginine and L-threonine. A two-part solid phase extraction was used to separate nitrogen heterocycles from byproducts followed by prep­ HPLC and the Ames mutagenicity assay using Salmonella TA98 to determine the mutagenicity of various isolates. The results indicate that this binary amino acid mixture does not yield a mutagenic sample.

The Impact Of Male-Exposure In The Neuronal Response Of The L3 Auditory Intemeuron To Model Calls, Shekinah Dosunmu

Honors Theses

The L3 auditory neuron in female cricket Acheta domesticus responds to phonotactically attractive calls with decrement: a reduction in the number of action potentials in response to consecutive sound pulses within a chirp. Such unique response has been reported only in virgin females. This study evaluates the response of the L3 in male-exposed females to determine whether they respond to auditory stimuli differently from those exhibited by virgin female crickets.

Preliminary data indicate that L3 in young exposed female crickets responds with different decrement patterns as those reported for virgin females. The implications of these findings are discussed.

Synthesis Of An Ideal Inhibitor For The Lpxc Enzyme, Taejun Lee

Honors Theses

Due to the growing need of antibiotics in modern day medicine, this study attempts to apply a method of medicinal chemistry that would create a novel inhibitor for a lesser known target of gram negative bacteria, the LpxC enzyme. This study proposes that a synthetic plan which consists of combining components of different molecules would create a more effective inhibitor. These components include a binding group for the active site of the enzyme, a hydrophobic chain which has been shown to interact with an allosteric site on the LpxC enzyme, and the isoxazoline ring which interacts with the zinc ion. …

Neuronal And Behavioral Response Relationships In Virgin Females Of Acheta Domesticus, Brandon Shin

Honors Theses

Animals that communicate acoustically serve as models for investigating how organisms respond to sound. For instance, in field crickets, females exhibit phonotaxis (directed movement towards the sound's source) to calls with a specific frequency, intensity, and syllable period (SP) - the time between the beginning of one sound pulse and the beginning of another within the same chirp. Additionally, auditory neurons in females produce action potentials in direct response to the sound pulses of a male 's call. A previous study on the species Gryllus bimaculatus demonstrated a relationship between an individual female's behavioral and neuronal responses; however, no such …

A Note On The Onset Of Synchronous Egg Laying In A Seabird Behavior Model, Dorothea Gallos, Christiane Gallos

Honors Theses

Protection Island, Washington hosts a large colony of Glaucous-winged gulls (Larus glaucescens). These birds are known to exhibit every-other-day egg-laying synchrony in dense areas of the colony in response to egg cannibalism, which is the main source of egg loss. Here we present an equilibrium analysis of a discrete-time animal behavior model for egg laying. We use Jury Conditions to find the stability criteria for the equilibrium as a function of the colony density and show that a 2-cycle bifurcation occurs when the equilibrium loses stability. The 2-cycle pattern in egg laying becomes increasingly synchronous as the colony density increases. …

Evaluating Mutagenicity Of Burned Arginine-Based Heterocyclic Amines And Anti-Mutagenicity Effect Of Chinese Medicinal Herbs, Rayford C. Alva

Honors Theses

From the unexpected finding that cooked grains and meat substitutes elicit a mutagenic response in Salmonella typhimurium TA98, our work has been aimed at deconstructing this finding via a survey of heated binary amino acid combinations, involving arginine, a plant-based amino acid. secondly, our work has looked towards sing phytochemical extracts from traditional Chinese medicinal herbs to inhibit the mutagenic activity of heterocyclic amines (main culprit for mutagenicity in meat). Two fractions from the burned products of arginine and phenylalanine (RF-HCA-06 and -07) were statistically significant inducers of mutagenesis; Scutellaria barbata and Oldenlandia diffusa, both separately and together, were statistically …