Life Sciences Commons^™

Deep Learning Modeling M6a Deposition Reveals The Importance Of Downstream Cis-Element Sequences., Zhiyuan Luo, Jiacheng Zhang, Jingyi Fei, Shengdong Ke

Faculty Research 2022

The N⁶-methyladenosine (m⁶A) modification is deposited to nascent transcripts on chromatin, but its site-specificity mechanism is mostly unknown. Here we model the m⁶A deposition to pre-mRNA by iM6A (intelligent m⁶A), a deep learning method, demonstrating that the site-specific m⁶A methylation is primarily determined by the flanking nucleotide sequences. iM6A accurately models the m⁶A deposition (AUROC = 0.99) and uncovers surprisingly that the cis-elements regulating the m⁶A deposition preferentially reside within the 50 nt downstream of the m⁶A sites. The m⁶A enhancers mostly …